Comparison of suspension MDCK cells, adherent MDCK cells, and LLC‐MK2 cells for selective isolation of influenza viruses to be used as vaccine seeds

Abstract

Background: Cell-based influenza vaccines can solve the problem of the frequent occurrence of egg adaptation-associated antigenic changes observed in egg-based vaccines. Seed viruses for cell-based vaccines can be prepared from clinical specimens by cell culture; however, clinical samples risk harboring respiratory viruses other than influenza virus. Therefore, it is necessary to investigate the patterns of co-infection in clinical samples and explore whether cell culture technology can selectively propa-

Viral Coinfections

“…The disadvantage of this method is whether or not some traditional virus isolation cell lines are sensitive to another virus, and coinfection may affect the formation of CPE. At present, it is feasible to separate snakehead retrovirus (SnRV) from grouper nervous necrosis virus (GNNV) by SGF-1 [165]; FMDV from PPRV [130] or single serotype FMDV from multiple serotypes ofFMDV [142] by BHK21; IAV from respiratory viruses by suspended MDCK cells (MDCK-S) and adherent MDCK cells (MDCK-A) [166];…”