Comparison of the effect of phenol and its derivatives on protein and free radical formation in human erythrocytes (in vitro)

Bukowska, Bożena; Michałowicz, Jaromir; Krokosz, Anita; Sicińska, Paulina

doi:10.1016/j.bcmd.2007.06.003

Cited by 46 publications

(21 citation statements)

References 34 publications

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“…Organic xenobiotics, similarly to other contaminants, may accelerate the formation of oxy-radicals in plants. In some investigations it has also been proved that aromatic compounds like chlorophenols may induce ROS formation in living cells (Bukowska et al 2007). Moreover, it was shown that precursors of chlorophenols, like the herbicide 2,4-dichlorophenoxyacetic acid, are able to produce ROS like hydrogen peroxide in leaves (Romero-Puertas et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Chloroguaiacols Change Some Antioxidative Parameters and Affect the Activity of Glutathione S-transferase in the Leaves of Reed Canary Grass (Phalaris arudinacea)

Michałowicz¹,

Urbanek²,

Bukowska³

et al. 2009

Water Air Soil Pollut

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In this work, we evaluated the effect of harmful ecotoxins, 4,5,6-trichloroguaicol (4,5,6-TCG) and tetrachloroguaiacol (TeCG), on the oxidation of the fluorescent probe dihydrorhodamine 123, the content of free phenols and the level of the total, oxidized and reduced glutathione in the leaves of reed canary grass (Phalaris arudinacea). Furthermore, the effect on the activity of guaiacol peroxidase and glutathione S-transferase was investigated. Both 4,5,6-TCG and TeCG increased the activity of guaiacol peroxidase and glutathione S-transferase, they also elevated the content of free phenols and the level of the total glutathione. A stronger effect was exerted by tetrachloroguaiacol, which strongly increased the level of the total glutathione and the content of free phenols on the 3rd and 6th day of the experiment. The activity of glutathione S-transferase was more intensively induced by trichloroguaiacol. Both 4,5,6-TCG and TeCG oxidized dihydrorodamine 123 and the effect was stronger in the presence of magnesium ions.

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Section: Discussionmentioning

confidence: 99%

Chloroguaiacols Change Some Antioxidative Parameters and Affect the Activity of Glutathione S-transferase in the Leaves of Reed Canary Grass (Phalaris arudinacea)

Michałowicz¹,

Urbanek²,

Bukowska³

et al. 2009

Water Air Soil Pollut

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“…Changes in the lipid organization indicate changes in the physicochemical properties of the cell lipid bilayer membrane by, e.g., reduction in hydrophobicity of the lipid membrane interior, change in organization and fluidity of the bilayer and disorders of the lipid asymmetry and depolarization of the membrane [19][20][21]. As a consequence of those changes, an inhibition of the activity of the enzyme and transport of proteins and loss of membrane integrity may occur, leading to cell's death.…”

Section: Introductionmentioning

confidence: 99%

Application of the DSC and spectroscopy methods in the analysis of the protective effect of extracts from the blueberry fruit of the genus Vaccinium in relation to the lipid membrane

Pruchnik

Bonarska-Kujawa

Żyłka

et al. 2018

J Therm Anal Calorim

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The aim of the study was to determine changes incurred in lipid model membranes by polyphenol compounds from blueberry fruit of the genus Vaccinium that includes low, high and black blueberry. In particular, the effect of extracts on the packing order in the lipid hydrophilic phase, the fluidity of the hydrophobic phase, as well as the temperature of the phase transition in phospholipids was studied. Model membranes were formed of dipalmitoylphosphatidylcholine (DPPC) and egg phosphatidylcholine. The interaction of extracts with lipids was studied using the differential scanning calorimetry, infrared spectroscopy (ATR-FTIR) and fluorimetry using Laurdan, Prodan and DPH probes. All experimental results indicate that the biggest changes are in the hydrophilic part of the lipid bilayer. The polyphenol compounds had practically no influence on fluidity in the hydrophobic region of the membranes. No changes in the temperature of the main phase transition of DPPC were observed and only a small change in the temperature of pretransition for high concentration of compounds. Results obtained with the ATR-FTIR method did not reveal any changes in the alkyl chain region of bilayer; however, a small shift of bands was observed for phosphate and choline groups-the broadest effect appeared for the wild bilberry. The findings indicate that polyphenolic compounds contained in extracts of blueberries interact with the membrane surface and therefore can protect it.

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“…The antioxidant activity of some flavonoids, including anthocyanin, is markedly higher than the activity of vitamin E, and its synthetic equivalent Trolox ® . They also effectively inhibit erythrocyte hemolysis induced by free radicals, protecting the erythrocyte membrane against oxidative destruction [5,10,[13][14][15][16][17][18]]. An exposed and very good site of attack by free radicals in an organism is the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Interaction of selected anthocyanins with erythrocytes and liposome membranes

Bonarska-Kujawa

Pruchnik

Kleszczyńska

2012

Cellular and Molecular Biology Letters

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Anthocyanins are one of the main flavonoid groups. They are responsible for, e.g., the color of plants and have antioxidant features and a wide spectrum of medical activity. The subject of the study was the following compounds that belong to the anthocyanins and which can be found, e.g., in strawberries and chokeberries: callistephin chloride (pelargonidin-3-O-glucoside chloride) and ideain chloride (cyanidin-3-O-galactoside chloride). The aim of the study was to determine the compounds' antioxidant activity towards the erythrocyte membrane and changes incurred by the tested anthocyanins in the lipid phase of the erythrocyte membrane, in liposomes composed of erythrocyte lipids and in DPPC, DPPC/cholesterol and egg lecithin liposomes. In particular, we studied the effect of the two selected anthocyanins on red blood cell morphology, on packing order in the lipid hydrophilic phase, on fluidity of the hydrophobic phase, as well as on the temperature of phase transition in DPPC and DPPC/cholesterol liposomes. Fluorimetry with the Laurdan and Prodan probes indicated increased packing density in the hydrophilic phase of the membrane in the presence of anthocyanins. Using the fluorescence probes DPH and TMA-DPH, no effect was noted inside the hydrophobic phase of the membrane, as the lipid bilayer fluidity was not modified. The compounds slightly lowered the phase transition temperature of phosphatidylcholine liposomes. The study has shown that both anthocyanins are incorporated into the outer region of the erythrocyte membrane, affecting its shape and lipid packing order, which is reflected in the increasing number of echinocytes. The investigation proved that the compounds penetrate only the outer part of the external lipid layer of liposomes composed of erythrocyte lipids, DPPC, DPPC/cholesterol and egg lecithin lipids, changing its packing order. Fluorimetry studies with DPH-PA proved that the tested anthocyanins are very effective antioxidants. The antioxidant activity of the compounds was comparable with the activity of Trolox ® .

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Comparison of the effect of phenol and its derivatives on protein and free radical formation in human erythrocytes (in vitro)

Cited by 46 publications

References 34 publications

Chloroguaiacols Change Some Antioxidative Parameters and Affect the Activity of Glutathione S-transferase in the Leaves of Reed Canary Grass (Phalaris arudinacea)

Chloroguaiacols Change Some Antioxidative Parameters and Affect the Activity of Glutathione S-transferase in the Leaves of Reed Canary Grass (Phalaris arudinacea)

Application of the DSC and spectroscopy methods in the analysis of the protective effect of extracts from the blueberry fruit of the genus Vaccinium in relation to the lipid membrane

Interaction of selected anthocyanins with erythrocytes and liposome membranes

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