Comparison of the effect of rose bengal- and eosin Y-mediated photodynamic inactivation on planktonic cells and biofilms of Candida albicans

Freire, Fernanda; Costa, Anna Carolina Borges Pereira; Pereira, Cristiane Aparecida; Beltrame, Milton; Junqueira, Juliana Campos; Jorge, Antônio Olavo Cardoso

doi:10.1007/s10103-013-1435-x

Cited by 50 publications

(46 citation statements)

References 30 publications

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“…Freire et al [34] compared Rose Bengal (RB) with Eosin Y (2,4,5,7 tetrabromofluorescein) assessing their potency for photoinactivation of Candida albicans biofilms. After culturing these biofilms for 48 h, treatment was done with 200 μM RB or Eosin Y for 5 min as a pre-irradiation period and subsequent illumination with a green LED (90mW; 532±10nm) for 180s (light dose: 42.63J/cm2; applied energy: 16.2J), which resulted in inactivation rates of 0.22 and 0.45 log 10 for RB and Eosin Y, respectively.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic therapy of oral Candida infection in a mouse model

Freire

Ferraresi

Jorge

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Species of the fungal genus Candida, can cause oral candidiasis especially in immunosuppressed patients. Many studies have investigated the use of photodynamic therapy (PDT) to kill fungi in vitro, but this approach has seldom been reported in animal models of infection. This study investigated the effects of PDT on Candida albicans as biofilms grown in vitro and also in an immunosuppressed mouse model of oral candidiasis infection. We used a luciferase-expressing strain that allowed noninvasive monitoring of the infection by bioluminescence imaging. The phenothiazinium salts, methylene blue (MB) and new methylene blue (NMB) were used as photosensitizers (PS), combined or not with potassium iodide (KI), and red laser (660 nm) at four different light doses (10J, 20J, 40J and 60J). The best in vitro log reduction of CFU/ml on biofilm grown cells was: MB plus KI with 40J (2.31 log; p < 0.001); and NMB without KI with 60J (1.77 log; p < 0.001). These conditions were chosen for treating the in vivo model of oral Candida infection. After 5 days of treatment the disease was practically eradicated, especially using MB plus KI with 40J. This study suggests that KI can potentiate PDT of fungal infection using MB (but not NMB) and could be a promising new approach for the treatment of oral candidiasis.

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Section: Discussionmentioning

confidence: 99%

Photodynamic therapy of oral Candida infection in a mouse model

Freire

Ferraresi

Jorge

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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“…However, it has to be considered that blue light from a 455 nm LED may not be optimal for excitation of ERY and RB with green light being more appropriate (Pereira et al, 2013). In a study from the same group, Freire et al (2013) compared RB with Eosin Y (2,4,5,7-tetrabromofluorescein) concerning their potency for inactivation of Candida albicans biofilms. After culturing these biofilms for 48 h, treatment was done with 200 μ M RB or Eosin Y for 5 min as a pre-irradiation period and subsequent illumination with a green LED (90 mW; 532±10 nm) for 180 s (light dose: 42.63 J/cm 2 ; applied energy: 16.2 J), which resulted in inactivation rates of 0.22 and 0.45 log 10 for RB and Eosin Y, respectively (Freire et al, 2013).…”

Section: Apdt Against Biofilms In Vitromentioning

confidence: 99%

“…In a study from the same group, Freire et al (2013) compared RB with Eosin Y (2,4,5,7-tetrabromofluorescein) concerning their potency for inactivation of Candida albicans biofilms. After culturing these biofilms for 48 h, treatment was done with 200 μ M RB or Eosin Y for 5 min as a pre-irradiation period and subsequent illumination with a green LED (90 mW; 532±10 nm) for 180 s (light dose: 42.63 J/cm 2 ; applied energy: 16.2 J), which resulted in inactivation rates of 0.22 and 0.45 log 10 for RB and Eosin Y, respectively (Freire et al, 2013). In contrast to those results, Kishen et al achieved a high killing efficacy of approximately 5 log 10 steps, when using RB against Enterococcus faecalis monospecies biofilms, as mentioned above (see Chapter Phenothiazinium Derivatives) (Kishen et al, 2010).…”

Section: Apdt Against Biofilms In Vitromentioning

confidence: 99%

Antimicrobial photodynamic therapy for inactivation of biofilms formed by oral key pathogens

Cieplik¹,

Tabenski²,

Buchalla³

et al. 2014

Front. Microbiol.

201

137

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With increasing numbers of antibiotic-resistant pathogens all over the world there is a pressing need for strategies that are capable of inactivating biofilm-state pathogens with less potential of developing resistances in pathogens. Antimicrobial strategies of that kind are especially needed in dentistry in order to avoid the usage of antibiotics for treatment of periodontal, endodontic or mucosal topical infections caused by bacterial or yeast biofilms. One possible option could be the antimicrobial photodynamic therapy (aPDT), whereby the lethal effect of aPDT is based on the principle that visible light activates a photosensitizer (PS), leading to the formation of reactive oxygen species, e.g., singlet oxygen, which induce phototoxicity immediately during illumination. Many compounds have been described as potential PS for aPDT against bacterial and yeast biofilms so far, but conflicting results have been reported. Therefore, the aim of the present review is to outline the actual state of the art regarding the potential of aPDT for inactivation of biofilms formed in vitro with a main focus on those formed by oral key pathogens and structured regarding the distinct types of PS.

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“…Eosin Y was selected as a photosensitizer because it absorbs blue light and was previously shown to inactivate Lactobacilli and Candida albicans upon light activation [14,15]. Intermittent irradiation consisted in exposing pathogens to light and photosensitizer first, then re-incubating pathogens for a certain period of time before repeating the irradiation.…”

Section: Introductionmentioning

confidence: 99%

Repeated exposures to blue light-activated eosin Y enhance inactivation of E. faecalis biofilms, in vitro

Marinic

Manoil

Filieri

et al. 2015

Photodiagnosis and Photodynamic Therapy

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Background: In dentistry, antibacterial photodynamic therapy (a-PDT) has shown promising results for inactivating bacterial biofilms causing carious, endodontic and periodontal diseases. In the current study, we assessed the ability of eosin Y exposed to 3 irradiation protocols at inactivating Enterococcus faecalis biofilms, in vitro. Methods: E. faecalis biofilms formed on hydroxyapatite disks were incubated with eosin Y (10-80 M), then activated with blue light using different irradiation protocols. Biofilms exposed to continuous exposure were incubated for 40 min before being light-activated for 960 s. For the intermittent exposure, biofilms were exposed 4 times to the light/photosensitizer combination (960 s total) without renewing the photosensitizer. For repeated a-PDT, the same light dose was delivered in a series of 4 irradiation periods separated by dark periods; fresh photosensitizer was added between each light irradiation. After treatment, bacteria were immediately labeled with LIVE/DEAD BacLight Bacterial Viability kit and viability was assessed by flow cytometry (FCM). Results were statistically analyzed using one-way ANOVA and Tukey multiple comparison intervals (˛= 0.05). * Corresponding author. Results:The viability of E. faecalis biofilms exposed to 10 M eosin Y, was significantly reduced compared to controls (light only-eosin Y only). After a second exposure to blue light-activated eosin Y, viability significantly decreased from 58% to 12% whereas 6.5% of the bacterial biofilm remained live after a third exposure (p < 0.05). Only 3.5% of the bacterial population survived after the fourth exposure. Conclusions:The results of this study indicate that blue light-activated eosin Y can photoinactivate E. faecalis biofilms grown on hydroxyapatite disks. Also, repeated exposures to blue light-activated eosin Y were shown to significantly improve efficacy. Further studies seem warranted to optimize the antibacterial activity of blue light-activated eosin Y on major oral pathogens.

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Comparison of the effect of rose bengal- and eosin Y-mediated photodynamic inactivation on planktonic cells and biofilms of Candida albicans

Cited by 50 publications

References 30 publications

Photodynamic therapy of oral Candida infection in a mouse model

Photodynamic therapy of oral Candida infection in a mouse model

Antimicrobial photodynamic therapy for inactivation of biofilms formed by oral key pathogens

Repeated exposures to blue light-activated eosin Y enhance inactivation of E. faecalis biofilms, in vitro

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