Comparison of the Effects of Primary Prostaglandins on Isolated Human Urinary Bladder

Abstract

The effects of primary prostaglandins (PGs) on the dome and trigone of the human urinary bladder were investigated in vitro. In the dome preparations, PGE1 (10(-9) to 10(-6) gm./ml.) showed a weak contractile effect, while PGE2 (10(-9) to 10(-6) gm./ml.) produced dose-dependent contractions. PGF2 alpha (10(-9) to 10(-6) gm./ml.) induced contractions greater than those induced by PGE1 and PGE2. In the trigone preparations, PGE1 (10(-9) to 10(-6) gm./ml.) had no effect, and PGE2 (10(-9) to 10(-6) gm./ml.) produced only a weak contractile response. PGF2 alpha (10(-9) to 10(-6) gm./ml.) showed a greater contractile potency than PGE2. Contractile responses of both preparations to PGE1, PGE2 and PGF2 alpha were not affected by pretreatments of atropine, hexamethonium and phentolamine. These results suggest that the order of potency of primary PGs to induce contractile responses is PGF2 alpha greater than PGE2 greater than PGE1 in both the dome and the trigone preparations, and that the contractile effects of PGE1, PGE2 and PGF2 alpha may not be mediated by activation of muscarinic, nicotinic or adrenergic alpha-receptors.

Age-Related Changes in Contractile Force of Rabbit Detrusor Muscle to Prostaglandin E₁, E₂ and F_2α

“…A similar relationship has been re ported by Khanna et al [7] in in vivo rabbit detrusor smooth muscle and by Pogessi et al [8] in studies of the intravesical pressure elevation in the in vivo rabbit. In contrast, Ueda et al [10] and Abrams and Feneley [3] noted that the rank order of the magnitude of the con tractile effects of PGs on human detrusor muscle strips was PGF2« > PGE2 > PGE 1 at concentrations of 10-9 -10'6 g/ml. In the present study, the response to PGE| (10~6-1 0 -5 M) is greater in detrusor muscle from old than from young rabbits, whereas the responses to PGs E2 (10~7-10-5 M) and F2« (10-6-10-5 M) are greater in detrusor from young than from old rabbits.…”

“…There have been numerous studies on the effects of prostaglandins (PGs) on the smooth muscle of the uri nary bladder both in vivo and in vitro [1][2][3][4][5][6][7][8][9][10] and attempts have been made to evoke bladder contractions in man by the intravesical infusion of PGE2 [4,11], The effects of PGs are known to differ with age in human lymphocyte [12], rat testicular capsule [13] and in the colon, stomach and ileum of the guinea pig and rat [14], The effect of age on the response of urinary bladder smooth muscle to PGs has not been studied previously. The present study was designed to assess the age-dependent effects of PGs on rabbit detrusor smooth muscle.…”

Alteration of urothelial‐mediated tone in the ischemic bladder: Role of eicosanoids

“…PGs and LTs are di¡erentially synthesized in the urothelium and smooth muscle, respectively.Therefore, their natural balanced levels may be of great importance in maintaining the physiologic urothelial^mesenchymal interactions . It has been shown that the COX and 5-LO products are potent constrictors of the bladder smooth muscle where LTs appear more potent than PGs [Ueda et al, 1985;Bjorling et al, 1994]. In certain cases, PGs and LTs have shown opposing e¡ects under the same pathologic condition.…”

“…Urothelial regulation of bladder smooth muscle contractility has frequently been reported [Ueda et al, 1985;Bjorling et al, 1994;Krasnopolsky et al, 1995]. LTs, the 5-lipoxygenase (5-LO) products and prostaglandins (PGs), and the cyclooxygenase (COX) products of arachidonate have been shown to play important roles [Ueda et al, 1985;Bjorling et al, 1994].…”

“…LTs, the 5-lipoxygenase (5-LO) products and prostaglandins (PGs), and the cyclooxygenase (COX) products of arachidonate have been shown to play important roles [Ueda et al, 1985;Bjorling et al, 1994]. While COX-1 is expressed constitutively in many normal tissues, COX-2 has been shown to be expressed in both constitutive and inducible forms in the bladder [Smith and DeWitt, 1995].…”