2016
DOI: 10.18869/acadpub.rmm.4.2.15
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Comparison of the Lipophosphoglycan 3 Gene of the Lizard and Mammalian Leishmania: A Homology Modeling

Abstract: Background: Lipophosphoglycan 3 (LPG3) is required for the LPG assembly, a well known virulent molecule. In this study, the LPG3 gene of the lizard and mammalian Leishmania species were cloned and sequenced. A three-dimensional structure (3D) for the target sequence was also predicted by comparative (homology) modeling. Materials and Methods: An optimization PCR amplification was performed on genomic DNA extracted from two species of Leishmania. The desired PCR products were then cloned and sequenced. In addit… Show more

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