Comparison of the secretory process in the parotid and sublingual glands of the mouse

Vreugdenhil, Angie; Roukema, P.A.

doi:10.1016/0005-2736(75)90060-7

Cited by 32 publications

(17 citation statements)

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“…Although there have been many investigations on the effects of adrenergic and cholinergic agonists on the release of amylase and K+ from the salivary glands of several species (VREUGDENHIL and ROUKEMA, 1975;BOGART and PICARELLI, 1978;KAUFMAN, 1978;KAUFMAN and WONG, 1983), the reports in which the release of sialic acid from the salivary gland is studied are few. DOUGLAS and PoISNER (1964) and SAMLI and GESCHWIND (1968) reported that high K} concentrations in the medium provoked the release of vasopressin and luteinizing hormone from pituitary gland.…”

Section: Discussionmentioning

confidence: 99%

“…Secretion via the cholinergic or a-adrenergic mechanism requires extracellular Cat +, but the 3-adrenergic mechanism does not. In addition, the 3-adrenergic mechanism requires the increase of cyclic AMP level in the tissue, but the a-adrenergic or cholinergic mechanism does not (SCHRAMM and SELINGER, 1975 ;VREUGDENHIL and ROUKEMA, 1975 ;LESLIE et al, 1976). Thus, the mechanism of amylase and K+ releases has been elucidated to a certain extent.…”

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confidence: 99%

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Stimulation of amylase and sialic acid releases from dog submandibular gland slices by pilocarpine or high K+ medium: A possible role of calmodulin for their releases.

et al. 1983

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The mechanism of amylase and sialic acid releases stimulated by pilocarpine or high K+ medium was investigated in the slices of dog submandibular glands. The release of both amylase and sialic acid was dose-dependently increased by pilocarpine and a considerable release was observed at pilocarpine concentrations of more than 1 /tM. Similar effects were observed when K+ concentration in the medium was increased and the maximal response was observed at 75 mM K+. The release of amylase and sialic acid by pilocarpine or K+ considerably decreased by removing Ca2+ from the medium and the slices. The release of amylase in the Ca2 + -deficient slices was nearly recovered by the addition of 2.5 and 5.0 mM Ca2+, whereas that of sialic acid was recovered by only 60-75 %. Ca2+ inhibitors, La3+ and verapamil, and calmodulin inhibitors, trifluoperazine, prenylamine, and W-7, significantly inhibited the release of amylase and sialic acid induced by the stimulants. These results suggest that the release of amylase and sialic acid stimulated by pilocarpine or K+ is dependent on the presence of Ca2+, and that the activation of calmodulin is involved in the process of the release.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Stimulation of amylase and sialic acid releases from dog submandibular gland slices by pilocarpine or high K+ medium: A possible role of calmodulin for their releases.

et al. 1983

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“…In the sublingual glands adrenergic nerves are almost absent (Vreugdenhil & Roukema, 1975) and neither mucin secretion, nor adenylate cyclase activity are stimulated significantly by adrenergic drugs. Likewise, cholinergic agonists, which stimulate mucin secretion very well, have no influence on adenylate cyclase in glandular homogenates and membrane preparations.…”

Section: Introductionmentioning

confidence: 99%

“…The procedure for measuring the secretion in vitro was performed as described previously (Nieuw Amerongen et al 1978). For the determination of cyclic AMP in vitro, an appropriate number of unsliced glands was incubated as described by Vreugdenhil & Roukema (1975). During the incubation period, both tissue samples and 500 ,l. samples of the incubation fluid were taken at various time intervals and immediately frozen in liquid nitrogen.…”

Section: In Vitro Experimentsmentioning

confidence: 99%

“…At various time intervals after injection, the mice were decapitated. The sublingual glands were removed, rapidly cleaned in a cold saline solution, and either immediately frozen in liquid nitrogen for the cyclic AMP assay according to Brown, Albano, Ekins, Sgherzi & Tampion (1971), or stored at -20 'C for the amylase assay, which was performed after homogenization of the glands, as described previously (Vreugdenhil & Roukema, 1975).…”

Section: In Vivo Experimentsmentioning

confidence: 99%

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Cyclic AMP in the sublingual glands of the mouse.

Amerongen¹,

Roukema²,

Vreugdenhil³

1980

The Journal of Physiology

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SUMMARY 1. The cyclic AMP level in the sublingual glands of the mouse has been determined in relation to mucin secretion under the influence of several agonists in vivo and in vitro.2. Isoprenaline increased the cyclic AMP level in these glands only in the presence of a phosphodiesterase inhibitor, indicating the presence of an active cyclic AMP phosphodiesterase.3. Inhibition of phosphodiesterase results in an increase of the cyclic AMP levels. EGTA prolonged the effect of the PDE-inhibitors, indicating that Ca2+-ions may be involved in the maintenance of the cyclic AMP concentration in the sublingual glands.4. NaF is able to induce both a slight increase of the cyclic AMP level and a significant mucin secretion by the sublingual glands. However, other secretagogues do not significantly influence the cyclic AMP concentration in these glands, and compounds which do elevate its level, do not significantly stimulate sublingual mucin secretion.5. These data suggest that there is no direct relationship between cyclic AMP and sublingual mucin secretion.

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Salivary Mucin Secretion

Quissell

Tabak

1989

Comprehensive Physiology

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Comparison of the secretory process in the parotid and sublingual glands of the mouse

Cited by 32 publications

References 32 publications

Stimulation of amylase and sialic acid releases from dog submandibular gland slices by pilocarpine or high K+ medium: A possible role of calmodulin for their releases.

Stimulation of amylase and sialic acid releases from dog submandibular gland slices by pilocarpine or high K+ medium: A possible role of calmodulin for their releases.

Cyclic AMP in the sublingual glands of the mouse.

Salivary Mucin Secretion

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