Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media

Abstract

Two acidic polysaccharide fractions, CM-jd-CPS2 and CM-jd(Y)-CPS2, were isolated from the fruiting bodies of cultured Cordyceps militaris grown on solid rice medium and silkworm pupa, respectively, by hot-water extraction, ethanol precipitation and fractionation using ion-exchange column (DEAE-cellulose-52) and gel-filtration column (Sephadex G-100) chromatography. Their structural characterizations were performed by gas chromatography and fourier-transform infrared spectroscopy. Some differences existed between their structures, which indicated that culture media could influence the structure of polysaccharides of C. militaris. The antioxidant activities of CM-jd-CPS2 and CM-jd(Y)-CPS2 were evaluated by various methods in vitro. They had strong 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity and ferrous ion-chelating capacity, but moderate reducing power. The antioxidant activities of CM-jd(Y)-CPS2 were slightly higher than those of CM-jd-CPS2. These two acidic fractions were evaluated for proliferation of mouse splenocyte activity in vitro. They both possessed does-dependent mitogenic effects on mouse splenocytes, and could synergistically promote murine T- and B-lymphocytes induced by Con A and LPS. CM-jd(Y)-CPS2 exhibited stronger stimulatory activities upon immunomodulation than CM-jd-CPS2. These results are beneficial for the interpretation of the connection between polysaccharide structures and their biological activities.

Genotypic analysis of degenerative Cordyceps militaris cultured in the pupa of Bombyx mori

“…). C. militaris is commonly used in clinical practice in place of C. sinensis due to their similarities in chemical composition and pharmacological effects (Wu et al ).…”

Cordyceps militaris polysaccharide triggers apoptosis and G0/G1 cell arrest in cancer cells

“…Our previous results showed that the structure of C. militaris polysaccharides cultured by B. mori pupa is linked by α-glycosidic linkage, while that cultured by rice is linked by β-glycosidic linkage. Further study evaluated that the polysaccharides cultured by B. mori pupa exhibited stronger stimulatory activities upon immunomodulation than that cultured by rice (Wu et al, 2012). It agreed that polysaccharides with strong antitumor action differ greatly in their chemical structures.…”

“…Modern studies have reported to have beneficial biological activities such as immunomodulatory (Kuo et al, 2001;Wu et al, 2012), antioxidant (Yu et al, 2007), antitumor (Lin and Chiang, 2008;Park et al, 2009), anti-inflammatory (Won and Park, 2005), and anti-metastatic effect (Nakamura et al, 1999), etc. Although many studies have shown the antitumor properties of C. militaris (artificial cultivated from Bombyx mori pupa) polysaccharides, little is known regarding the mechanism of its effects. In this study, several cancer cells SMMC-7721 (liver), BGC-823 (stomach) and were conducted to determine the mechanism of antitumor effects of C. militaris polysaccharides by evaluating apoptosis rate and cell cycle progression status.…”

Purification and structural characterization of an α-glucosidase inhibitory polysaccharide from apricot ( Armeniaca sibirica L. Lam.) pulp

“…The bands in the 3430.07 and 1606.59 cm −1 regions corresponded to acylamino, which showed that APPS1-2 was a glycoconjugate containing polysaccharide and protein. The wave numbers between 800 and 1200 cm −1 represent the fingerprint region for carbohydrates (Kumar, Joo, Choi, Koo, & Chang, 2004;Matsuhiro, Osorio-Roman, & Torres, 2012;Wu et al, 2012). The absorption band at 1130.86 cm −1 corresponded to the C O C stretching vibration.…”