In this work, superconducting YBa2Cu3O7 (YBCO) thin films were grown on different substrates under the same conditions and their behaviours were investigated. At a higher substrate temperature, because of the occurrence of a chemical reaction between the substrate and film, the film quality was impaired, especially in case of the Al2O3 (sapphire) substrate. Films were also deposited onto other substrates, SrTiO3, MgO and ZrO2, and the effects of these substrates on the film quality were compared by x-ray diffraction.