Comparison of thin-layer chromatography, spectrofluorimetry and bright greenish-yellow fluorescence test for aflatoxin detection in corn

Ono, Elisabete Yurie Sataque; Silva, Marcelo da; Ribeiro, Ricardo Marcelo Reche; Ono, Mário Augusto; Hayashi, Luciana; Garcia, Glauco Tironi; Hirooka, Elisa Yoko

doi:10.1590/s1516-89132010000300025

Cited by 12 publications

(6 citation statements)

References 13 publications

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“…For trace detection of mycotoxins in complex matrices, many analytical methods, such as thin layer chromatography, enzyme‐linked immunosorbent assay, liquid chromatography and some emerging techniques, have been established. However, these methods either exhibited low sensitivity, poor repeatability, self‐quantitation determination with false‐positive results, or are ongoing that have not got wide application in a large number of samples.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 19 mycotoxins in lotus seed using a multimycotoxin UFLC-MS/MS method

Fang

Liu

Liao

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives In relevance to the internal components and improper environmental conditions, lotus seeds are susceptible to fungal contamination and mycotoxins residue, leading to harmful impacts on the quality and safety, as well as their pharmaceutical efficacy and clinical use. It is necessary and urgent to assess various mycotoxins residue in lotus seeds. This study aimed to develop a sensitive method for accurate assessment of multimycotoxin residues in lotus seeds. Methods A simple and reliable modified ultrasonication-assisted extraction, QuEChERS purification based ultrafast liquid chromatography tandem mass spectrometry (UFLC-MS/MS) method was successfully developed for ultrasensitive determination of 19 multiclass mycotoxins in starch-rich lotus seeds. Four extraction modes and three clean-up sorbents for improving the recoveries of mycotoxins were optimized. Limits of detection (LODs) and quantification, linearity, precision, accuracy, and matrix effect were studied for method validation. For simultaneous qualitation and quantification, the 19 chemically diversified mycotoxins were well separated on a CAPCELL CORE C18 column (100 mm 9 2.1 mm, 2.7 lm) and detected in positive/negative electrospray ionization mode within 7 min. Key findings The validated method exhibited satisfactory linearity (r > 0.995), ultragood selectivity (LODs of 0.1-15.0 lg/kg), excellent precision (RSDs <13.0%) and convincing accuracy (recoveries between 79.4% and 131.6% with RSDs <14.4%). Matrix effect, between 54.5% and 113.6%, appeared especially for aflatoxins B 1 and B 2 , deoxynivalenol and T-2 toxins. Matrix-matched curvebased quantification showed that 26 (57.8%) out of 45 lotus seed samples were contaminated with one or more mycotoxins, and ochratoxin A, aflatoxin B 2 , aflatoxin B 1 and citrinin were the most prevalent mycotoxins. Conclusions This study reports for the first time the incidence of a wide range of 19 mycotoxins in lotus seeds and the proposed method will get broad application for more trace components in other complex matrices.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 19 mycotoxins in lotus seed using a multimycotoxin UFLC-MS/MS method

Fang

Liu

Liao

et al. 2019

Journal of Pharmacy and Pharmacology

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“…The occurrence of aflatoxins in Brazilian foodstuffs has been frequently reported, mainly in peanut and corn products at variable levels and incidences from up to 50% of the samples analyzed (Magrine et al, 2011;Ono et al, 2010;Rodriguez-Amaya and Sabino, 2002). High incidences of AFM 1 in fluid milk have also been described, but at levels usually below the limit (0.50 g/L) established by Brazilian regulations (Agência Nacional De Vigilância Sanitária, 2011).…”

Section: Introductionmentioning

confidence: 99%

Assessment of aflatoxin exposure using serum and urinary biomarkers in São Paulo, Brazil: A pilot study

Jager

Tonin

Baptista

et al. 2016

International Journal of Hygiene and Environmental Health

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“…The occurrence of aflatoxins in Brazilian foodstuffs has been frequently reported, mainly in peanut and corn grains at rates from up to 50% of the samples analyzed [ 10 , 11 ]. However, there is little information on the human exposure to aflatoxins as assessed by aflatoxin biomarkers in human urine in Brazil, except for two reports, one of them by Romero et al .…”

Section: Introductionmentioning

confidence: 99%

Determination of Urinary Biomarkers for Assessment of Short-Term Human Exposure to Aflatoxins in São Paulo, Brazil

Jager

Tonin

Souto

et al. 2014

Toxins

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In the present study, a longitudinal assessment was carried out to evaluate the short-term human exposure to aflatoxins in Pirassununga region, São Paulo, Brazil, by determination of urinary aflatoxins by a liquid chromatography coupled to mass sprectrometry (UPLC-MS/MS) method. Sixteen volunteers with ages ranging from 14 to 55 years old were instructed to collect the early morning first urine four times every three months, from June 2011 to March 2012, totaling 64 samples. Aflatoxin M1 (AFM1) was found in 39 samples (61%) at levels ranging from 0.19 to 12.7 pg·mg−1 creatinine (mean: 1.2 ± 2.0 pg·mg−1 creatinine). Residues of aflatoxins B1, B2, G1, G2 and aflatoxicol were not identified in any urine sample. No significant difference was found among the AFM1 mean levels in urine samples collected in the four sampling periods. The levels of AFM1 found in urine samples indicate a low short-term exposure of the population studied to aflatoxins through the diet, although further investigations are needed to assess other long-term biomarkers of exposure to AFB1.

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Comparison of thin-layer chromatography, spectrofluorimetry and bright greenish-yellow fluorescence test for aflatoxin detection in corn

Abstract: In this study the bright greenish-yellow fluorescence test, widely used by the corn milling industry

Cited by 12 publications

References 13 publications

Simultaneous determination of 19 mycotoxins in lotus seed using a multimycotoxin UFLC-MS/MS method

Simultaneous determination of 19 mycotoxins in lotus seed using a multimycotoxin UFLC-MS/MS method

Assessment of aflatoxin exposure using serum and urinary biomarkers in São Paulo, Brazil: A pilot study

Determination of Urinary Biomarkers for Assessment of Short-Term Human Exposure to Aflatoxins in São Paulo, Brazil

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