Comparison of Various Solvent Extracts and Major Bioactive Components from Portulaca oleracea for Antioxidant, Anti-Tyrosinase, and Anti-α-Glucosidase Activities

Abstract

Portulaca oleracea is a well-known species for traditional medicine and food homology in Taiwan. In traditional medicine, P. oleracea is also used to treat gastrointestinal disorders, liver inflammation, fever, severe inflammation, and headaches. We investigated antioxidant, anti-tyrosinase, and anti-α-glucosidase activities of various solvent extracts and major bioactive components from P. oleracea. Ethanol and acetone extracts showed potent DPPH, ABTS, and hydroxyl radical scavenging activities. Chloroform and n-hexane extracts displayed significant superoxide radical scavenging activity. Furthermore, ethyl acetate and acetone extracts of P. oleracea showed potent anti-tyrosinase and anti-α-glucosidase activities. Examined and compared to the various solvent extracts for their chemical compositions using HPLC analysis, we isolated seven major compounds and analyzed their antioxidant, anti-tyrosinase, and anti-α-glucosidase activities. Seven active compounds of P. oleracea, especially quercetin, rosmarinic acid, and kaempferol, exhibited obvious antioxidant, anti-tyrosinase, and anti-α-glucosidase activities. The molecular docking model and the hydrophilic interactive mode of tyrosinase and α-glucosidase revealed that active compounds might have a higher antagonistic effect than commonly inhibitors. Our result shows that the active solvent extracts and their components of P. oleracea have the potential as natural antioxidants, tyrosinase and α-glucosidase inhibitors. Our results suggest that the active solvent extracts of P. oleracea and their components have potential as natural antioxidants, tyrosinase and α-glucosidase inhibitors.

Ultrasound-assisted extraction, optimization, isolation, and antioxidant activity analysis of flavonoids from Astragalus membranaceus stems and leaves

“…However, antioxidants in the body fail to remove free radicals completely once the body’s immunity is weakened or invaded by toxic substances, such as Cd and Hg, and the intake of foods rich in antioxidants can make up for this deficiency. Notably, the extensive application of artificial antioxidants in the food industry may cause liver damage and cancer [9] . This study assessed the antioxidant and antifungal activities of A. membranaceus stem and leaf extracts to provide a possible innovative application for the development of natural antioxidants from A. membranaceus by-products, encourage recycling, and reduce the environmental impact of waste disposal.…”

“…At present, synthetic antioxidants, such as butylated hydroxy anisole (BHA) and butylated hydroxy toluene (BHT), are widely used in food production and processing. However, studies have shown that the widespread use of synthetic antioxidants may cause liver damage and cancer [9] . Therefore, natural food antioxidants with high safety, strong antibacterial ability, and absence of side effects have attracted considerable research attention.…”

Phytochemical Screening and In Vitro Evaluation of the Antioxidant Potential of Dichloromethane Extracts of Strychnos henningsii Gilg. and Ficus sycomorus L.

“…Based on phytochemical studies, the yield and the antioxidant activity of a plant extract depend on the selected extraction solvent [65][66][67]. Diferent solvents are employed to isolate diferent antioxidant compounds based on their disparities in polarities [68]. In this study, dichloromethane solvents were used as a midpolar solvent for extraction of polyphenols and other midpolar phytochemicals.…”

Fabrication of a Magnetic Porous Organic Polymer for α-Glucosidase Immobilization and Its Application in Inhibitor Screening

“…They are also the precious resource for new drug research and development. It is reported that many TCMs like mulberry leaves, Catechu , Portulaca oleracea, and Cornus officinalis exhibit good inhibitory activity on α-glucosidase. However, it is challenging to promptly and accurately discover the target active ingredients in complex TCMs.…”