Compatibility of Fungal and Bacterial Bio-Agents and their Antagonistic Effect against Fusarium oxysporum f. Sp. Lycopersici

shita, Har; Sinha, Asha; Khan, JB; Trivedi, Shubha Sagar; Verma, Ankur; Rao, S. Gunneswara

doi:10.20546/ijcmas.2018.707.269

Cited by 8 publications

(4 citation statements)

References 5 publications

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“…This study revealed that 11 interactions among Trichoderma and Pseudomonas strains were compatible with each other as no zone of inhibition was observed between the strains. Our findings were in agreement with [ 17 – 19 , 43 ], who reported that, the compatibility of Pseudomonas and Trichoderma species under in-vitro assays. In our study, the promising potential antagonistic Pseudomonas and Trichoderma strains displayed compatibility under in-vitro conditions.…”

Section: Discussionsupporting

confidence: 93%

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In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

2021

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Background Faba bean (Vicia faba L.) cultivation is highly challenged by faba bean black root rot disease (Fusarium solani) in high lands of Ethiopia. To ensure sustainable production of faba beans, searching for eco-friendly disease management options is necessary to curb the progress of the disease timely. The indigenous biocontrol agents that suit local environments may effectively strive with in-situ microorganisms and suppress local pathogen strains. This study aimed to screen antagonistic indigenous compatible Trichoderma and Pseudomonas strains against Fusarium solani. In the pathogenicity test, soil-filled pots were arranged in complete random block design and sown with health faba bean seeds. The effect of some fungicides was evaluated against Fusarium by food poisoning methods to compare with the biocontrol agents. The antagonistic efficacy of biocontrol agents and their compatibility was investigated on Potato dextrose agar medium. Results Fusarium solani AAUF51 strain caused an intense root rotting in faba bean plant. The effect of Mancozeb 80% WP at 300 ppm was comparable with Trichoderma and Pseudomonas strains against Fusarium. The mycelial growth of test the pathogen was significantly (P ≤ 0.05) reduced to 86.67 and 85.19% by Trichoderma harzianum AAUW1 and Trichoderma viridae AAUC22 strains in dual culture, respectively. The volatile metabolites of Pseudomonas aeruginosa AAUS31 (77.78%) found the most efficient in reducing mycelial growth of Fusarium followed by Pseudomonas fluorescens AAUPF62 (71.11%) strains. The cell-free culture filtrates of Pseudomonas fluorescens AAUPF62 and Pseudomonas aeruginosa AAUS31 were more efficient than the Trichoderma strain in reducing the growth of Fusarium isolates. There was no zone of inhibition recorded between Trichoderma harzianum AAUW1, Trichoderma viridae AAUC22, Pseudomonas aeruginosa AAUS31, and Pseudomonas fluorescens AAUPF62 strains, hence they were mutually compatible. Conclusions The compatible Trichoderma and Pseudomonas strains showed antagonistic potentiality that could be explored for faba bean protection against black root rot disease and might have a future dual application as biocontrol agents.

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Section: Discussionsupporting

confidence: 93%

“…The plant growth-promoting Pseudomonas aeruginosa have increased the yield by suppressing the disease effect [ 16 ]. However, the use of a single biocontrol agent often resulted in inconsistent performances in agriculture [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

2021

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“…This finding is in line with results reported in Yan et al ( 2004) and Moretti et al (2008), which showed that A. xylosoxidans was able to inhibit species belonging to these two genera. Harashita et al (2018) reported that Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fluorescens had an antagonistic activity against Fusarium oxysporum f.sp. lycopersici.…”

Section: Discussionmentioning

confidence: 99%

Biological and Chemical control of some Tomato Fungal Diseases

2021

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I N RESPONSE to environmental and health concerns about the extended use of pesticides, it exists a considerable interest in finding alternative control approaches. The current study compared the antifungal activities of some biocontrol agents. Three fungal strains were isolated from infected tomato. Two belonged to genus Aspergillus and the third was Fusarium sp. Ten morphologically different bacterial strains were isolated from intact tomato roots and rhizospheric soils, they were screened for their antagonistic effects against the fungal isolates. Achromobacter xylosoxidans produced the most potent antifungal activity as indicated by the percentage of fungal weight loss recorded (equivalent to 73, 77, and 99% for Aspergillus sp. 1, Fusarium sp., and Aspergillus sp. 2, respectively). Four tested plants: Inula (Dittrichia viscosa), Onion (Allium cepa), Basillic (Ocimum basilicum), and Quinoa (Chenopodium quinoa) were tested for their antifungal activities against the isolated fungal pathogens. Inula extract showed the highest inhibition of fungal growth among the tested plants. The MIC values were evaluated for the tested biocontrol agents and compared with the standard antifungal chemicals: Between and Logico. In vivo study was performed using seedlings of Tomato (Solanum lycopersicum). The seedlings infected with the fungal pathogens using spraying technique showed severe symptoms of fungal disease, while the tomato plants infected with fungal pathogens and treated with the biocontrol agents (cell-free supernatant of A. xylosoxidans or Inula extract) exhibited observed reduction in the percentage of disease severity.

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“…Absence of inhibition zone indicated that the biocontrol agents were compatible with each other. 17 Study of effect of pesticides comprise of imidaclopride, flufenoxuron, teflubenzuron+ phuzalon, endosulfane and amitraz on conidia germination, vegetative growth and sporulation of B. bassiana and test their compatibility too.The research found that B. bassiana is incompatible with flufenoxuron. Flufenoxuron was causal of complete inhibition in B. bassiana growing.…”

Section: Discussionmentioning

confidence: 99%

Interaction of Antagonistic Bacteria that Effective to Control Rice Bacterial Leaf Blight Disease with Agricultural Chemicals and Bio-products

Sutthisa¹,

Soparut²

2019

J Pure Appl Microbiol

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This research aimed to study the compatibility of antagonistic bacteria that effective to control rice bacterial leaf blight disease, including Bacillus pumilus FDKF5 and Bacillus sp. IKM1 with agricultural chemicals and bio-products. Compatibility test of antagonistic bacteria with pesticides by using poison plate method showed that B. pumilus FDKF5 and Bacillus sp. IKM1 were able grow together with eight pesticides including 5 types of insecticide such as Conbalis (pyridaben 20% WP), Centaur (imidachloprid 70% WG), Posch (carbosulfan 20% W/V EC), Microthiol sulfur (sulfur 80% WG) and Abamectin (abamectin 1.8% W/V EC) and 3 types of fungicide including Terraclor Super-X (etridiazole 6% + quintozene 4% W/V EC), Fundasol 50 (benomyl 50% WP) and Octave (prochloraz 50% WP). However, streptomycin sulfate bactericide was inhibited their growth. Co-culture test of antagonistic bacteria with bio-products by using dual culture technique showed that Trichoderma and Beauveria bio-products could overgrowth on colonies of antagonistic bacteria but did not inhibit their growth. While, Metarhizium bio-products could inhibit the growth of both antagonistic bacteria. Dual culture of antagonistic bacteria with Bacillus bio-product showed that Bacillus sp. IKM1 could grow very well while B. pumilus FDKF5 could grow moderately.

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Compatibility of Fungal and Bacterial Bio-Agents and their Antagonistic Effect against Fusarium oxysporum f. Sp. Lycopersici

Cited by 8 publications

References 5 publications

In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

Biological and Chemical control of some Tomato Fungal Diseases

Interaction of Antagonistic Bacteria that Effective to Control Rice Bacterial Leaf Blight Disease with Agricultural Chemicals and Bio-products

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