Competitive immunoassay for staphylococcal enterotoxin A using capillary electrophoresis with laser-induced fluorescence detection

Lam, Michael T.; Wan, Qian-Hong; Boulet, Camille A.; Le, X. Chris

doi:10.1016/s0021-9673(99)00677-9

Cited by 45 publications

(37 citation statements)

References 4 publications

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“…The pharmacokinetics of diverse drugs such as, antibiotics, expectorants, anti-inflammatory and analgesics has been quantified and monitored mostly by CE-LIFD [86][87][88][89][90][91]. CE-LIFD has been used for the other type of screening, such as carbohydrates in urine, cancer screening in urine [92,93], even for selective detection of unusual substances as bacterial enterotoxins [94]. Some other prescription drugs such as cloramphenicol, clozapine, and metronidazol, and body proteins, such as myoglobin have been analyzed by CE-EC in human blood or urine [95][96][97][98].…”

Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

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Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

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Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

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“…The analyte can therefore be determined based on comparing the concentration of Ag * (or Ab * ) and Ag * -Ab (or Ab * -Ag) complex. Since Nielsen first introduced CEIA in 1991 using a UV detector [21], CEIA techniques have been widely applied for the detection of proteins [22][23][24], peptides [25][26][27], hormones [28][29][30], and abused drugs [31][32][33][34] with the aid of a highly sensitive LIF detector. As labeled macromolecules (Ag or Ab) are generally used in CEIA, there are still some challenges in developing CEIA.…”

Section: Introductionmentioning

confidence: 99%

Antibody development to testosterone and its application in capillary electrophoresis‐based immunoassay

Chen

Zhang

2008

Electrophoresis

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The present report describes a rapid, simple, and highly selective approach to perform testosterone competitive immunoassay by CE and LIF detection. The method involves using synthesized fluorescence-labeled testosterone as a tracer to compete with testosterone. Two polyclonal antibodies (antibody (Ab) arised from T-3-BSA (Ab(3)) and Ab arised from T-17-BSA (Ab(17))) and their respective tracers have been optimized and Ab(3) system is used for the quantification of testosterone by CE-based immunoassay. The method is developed with a wide working range of 3.70-2000 ng/mL and an LOD at 1.11 ng/mL. Tests for normal and positive urine samples show that this method has the potential to be applied in testosterone doping control.

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“…Due to the significant difference of the electrophoretic mobility of the labeled free antigen from that of the corresponding immunocomplex, the separation and the peak quantification can be easily performed in a free zone CE mode independently by the charge to mass ratio of analytes. As a consequence, competitive formats are studied more often in CEIA [25,26].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis enzyme immunoassay for alpha‐fetoprotein and thyroxine in human serum with electrochemical detection

Zhang

2008

J of Separation Science

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A novel CE-based enzyme immunoassay (CE-EIA) method was developed in o-aminophenol (OAP)-H(2)O(2)-horseradish peroxidase (HRP) system and applied to benign liver disease and hyperthyroidism research in the clinical practical field. In the presented method, after the enzyme immunoreaction, the HRP-labeled antibody or HRP-labeled antigen catalyzed the enzyme substrate OAP and H(2)O(2). The product of the enzymatic catalysis reaction 2-aminophenoxazine-3-one (AP) was determined using electrochemical detection on a Pt electrode at the outlet of the reaction capillary. Factors influencing the performance, including running buffer concentration, separation, and detection voltage, were investigated to the optimum conditions. Noncompetitive and competitive models were utilized to detect alpha-fetoprotein (AFP) and thyroxine (T(4)) in human sera, respectively. The linear ranges and the detection limits (S/N = 3) were from 1.5 to 66.6 ng/mL and 0.48 ng/mL for AFP, and from 1.7 to 260.0 ng/mL and 1.0 ng/mL for T(4). The results of this method were linear proportional to those of spectrophotometric ELISA method, giving a good prospect for a new clinical diagnostic instrument.

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Competitive immunoassay for staphylococcal enterotoxin A using capillary electrophoresis with laser-induced fluorescence detection

Cited by 45 publications

References 4 publications

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Antibody development to testosterone and its application in capillary electrophoresis‐based immunoassay

Capillary electrophoresis enzyme immunoassay for alpha‐fetoprotein and thyroxine in human serum with electrochemical detection

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