Complete chloroplast genome sequencing of five Salix species and its application in the phylogeny and taxonomy of the genus

Abstract

In this study, whole chloroplast genomes of five Salix species (S. argyracea, S. dasyclados, S. eriocephala, S. integra 'Hakuro Nishiki', and S. suchowensis) were sequenced. These chloroplast genomes were 155 ,605, 155, 763, 155, 552, 155, 538, and 155 ,550 bp in length, harboring 131 genes (77 unigenes), 37 tRNA genes, 8 rRNA genes, and 86 mRNA genes, respectively. The genes ycf1, psaI, ycf2-2, rpoC2, rpl22, atpF, and ndhF were under positive selection among the 21 Salix species. psaI, ycf2-2, atpF, and ycf1-2 were under positive selection between the tree willow and shrub willow, and rpoC2, rpl22, and ycf1-2 were positively selected among the shrub genomes. The gene rps7 was most variable among the genomes. Phylogenetic analysis of 21 Salix species and Chosenia arbutifolia provide evidence that the cp genome data partially support the relationship with traditional taxonomic concepts in the Flora of China. This chloroplast genome elucidates Salix taxonomy and provides evidence for evolutionary research.

The complete chloroplast genome sequence of Salix kochiana Trautv. and its phylogenetic analysis

“…plastome to previously published data shows a high level of gene synteny with some publicly available Salix sequences (Zhou et al. 2021 ). Arabidopsis thaliana (NC_000932.1) was used as an outgroup.…”

“…The Salix genus is composed of ∼ 350–520 species that are distributed worldwide (Zhou et al. 2021 ). In China, the Salix genus contains 257 species, including 122 varieties and 33 variants, and they are distributed across provinces (Wang et al.…”

Comparative Analysis of Apopellia endiviifolia Plastomes Reveals a Strikingly High Level of Differentiation between Its Terrestrial and Water Form

“…More evidence of positive selection at genus level could be found in wider studied vascular plants. Both ycf genes were detected under positive selection in genus Fagopyrum [69], Holcoglossum [70] and Salix [71], while ycf 1 in Caragana [51]. The ccsA gene, which was pointed out as undergoing positive selection by all three tests, plays an important role in cytochrome biogenesis [72].…”

Phylogenetic analysis and complete chloroplast genome of Salix wilhelmsiana M.B.

“…The Salix genus, comprised of trees known for their world-renowned landscape greening and economic value, contains approximately 400 natural species distributed worldwide (Zhou et al. 2021 ). There are approximately 200 species found across provinces in China.…”