Complete Coding Genome Sequence for Mogiana Tick Virus, a Jingmenvirus Isolated from Ticks in Brazil

Villa, Erika; Maruyama, Sandra Regina; Miranda-Santos, Isabel K. F. de; Palacios, Gustavo; Ladner, Jason T.

doi:10.1128/genomea.00232-17

Cited by 35 publications

(37 citation statements)

References 11 publications

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“…Due to the development of molecular virology, high-throughput sequencing and viral metagenomics, many new flavivirus-like viruses have been discovered in recent decades (1,4,14). A large number of flavivirus-like viruses have been detected in or isolated from arthropods (1, 32, 33), but their ability to cause infection and disease in vertebrates and their medical significance are not known.…”

Section: Discussionmentioning

confidence: 99%

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First isolation and characterisation of Alongshan virus in Russia

Kholodilov

Litov

Klimentov

et al. 2019

Preprint

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AbstractIn recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmen tick virus (JMTV) group holds a special place among flavi-like viruses because, in contrast to the “classic” flaviviruses and other flavi-like viruses, they have a segmented ssRNA(+) genome. Two segments of the JMTV genome have homology with regions of the flavivirus genome encoding polymerase and helicase-protease proteins. JMTVs have several open reading frames (ORF) in segments encoding glycoprotein(s) and capsid protein and these ORF are specific only to them. JMTVs greatly differ in virion size.We isolated three strains of Alongshan virus (ALSV), which is a representative of the JMTV group, from adult Ixodes persulcatus ticks collected in two geographically-separated Russian regions in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMTV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream ORF in the glycoprotein-coding segment of ALSV and other members of the JMTV group.Significance StatementWe isolated three strains of Alongshan virus (ALSV) from adult Ixodes persulcatus ticks from two geographically separate areas of Russia in the Ixodes ricinus tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Our study confirmed the value of tick cell lines in virus isolation and maintenance of persistent infection. The majority of virions of the ALSV strain Miass527 were enveloped spherical particles with a diameter of 40.5±3.7 nm. We found evidence for the existence of a novel upstream ORF in the glycoprotein-coding segment of ALSV and other members of the Jingmen tick virus group.

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Section: Discussionmentioning

confidence: 99%

“…The geographic distribution of the JMTV group is very wide, encompassing Asia (1, 4,8,9), Europe (9)(10)(11)(12), Central and South America (2,3,(12)(13)(14) and Africa (2,12). Virus RNAs of this group were detected not only in different species of insects, e.g.…”

Section: Introductionmentioning

confidence: 99%

First isolation and characterisation of Alongshan virus in Russia

Kholodilov

Litov

Klimentov

et al. 2019

Preprint

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“…Positive sense RNA viruses were the most abundant (95%) and comprised viral genomes belonging to the Flaviviridae, Solemoviridae, and Tymoviridae families, while negative sense RNA viruses (5% of ssRNA viruses) were assigned to the Phenuiviridae and Chuviridae families ( Table 2). Among the Flaviviridae, the only viral genome detected was related to Mogiana tick virus, a tick-associated Jingmen virus primarily described in Rhipicephalus microplus ticks from Brazil [19,45]). As the genome of the Jingmen tick virus found in ticks from Guadeloupe and Martinique has already been characterized in a previous study, and serological screening in cattle blood has been performed [46], only the infection rates are described in this paper (see Section 3.2).…”

Section: Virome Composition Of Caribbean Cattle-associated Ticksmentioning

confidence: 99%

RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

Gondard

Temmam

Devillers

et al. 2020

Viruses

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Ticks transmit a wide variety of pathogens including bacteria, parasites and viruses. Over the last decade, numerous novel viruses have been described in arthropods, including ticks, and their characterization has provided new insights into RNA virus diversity and evolution. However, little is known about their ability to infect vertebrates. As very few studies have described the diversity of viruses present in ticks from the Caribbean, we implemented an RNA-sequencing approach on Amblyomma variegatum and Rhipicephalus microplus ticks collected from cattle in Guadeloupe and Martinique. Among the viral communities infecting Caribbean ticks, we selected four viruses belonging to the Chuviridae, Phenuiviridae and Flaviviridae families for further characterization and designing antibody screening tests. While viral prevalence in individual tick samples revealed high infection rates, suggesting a high level of exposure of Caribbean cattle to these viruses, no seropositive animals were detected. These results suggest that the Chuviridae- and Phenuiviridae-related viruses identified in the present study are more likely tick endosymbionts, raising the question of the epidemiological significance of their occurrence in ticks, especially regarding their possible impact on tick biology and vector capacity. The characterization of these viruses might open the door to new ways of preventing and controlling tick-borne diseases.

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“…The multicomponent (segmented) tick flaviviruses evolutionarily related to the unsegmented viruses of the genus Flavivirus have been recognized since only 2014 (1-4). This group of unclassified segmented tick-borne flaviviruses now includes Alongshan virus, Jingmen tick virus (JMTV) and Mogiana tick virus (MGTV), which have been detected in Asia, Europe and South America, respectively.…”

Section: Announcementmentioning

confidence: 99%

“…In five pools, we found Mogiana-like fragments using BLASTN. These fragments were aligned to the reference genome segments of Mogiana tick virus isolate MGTV/V4/11 (4). The average coverage of the four segments was 35, 18, 52, and 30, respectively.…”

Section: Announcementmentioning

confidence: 99%

Complete Coding Genome Sequence for a Novel Multicomponent Kindia Tick Virus Detected from Ticks Collected in Guinea

Протопопова

Shvalov

et al. 2020

Preprint

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Complete Coding Genome Sequence for Mogiana Tick Virus, a Jingmenvirus Isolated from Ticks in Brazil

Abstract: Mogiana tick virus (MGTV) is a segmented jingmenvirus isolated in 2011 from cattle ticks in Brazil. Here, we present a complete coding genome sequence for MGTV isolate MGTV/V4/11, including all four segments. MGTV is evolutionarily related to the Jingmen tick virus isolates SY84 and RC27.

Cited by 35 publications

References 11 publications

First isolation and characterisation of Alongshan virus in Russia

First isolation and characterisation of Alongshan virus in Russia

RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

Complete Coding Genome Sequence for a Novel Multicomponent Kindia Tick Virus Detected from Ticks Collected in Guinea

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