Complete Genome Sequence of a Type Strain of Mycobacterium abscessus subsp. bolletii , a Member of the Mycobacterium abscessus Complex

Abstract

Mycobacterium abscessus subsp. bolletii is a rapidly growing mycobacterial organism for which the taxonomy is unclear. Here, we report the complete genome sequence of a Mycobacterium abscessus subsp. bolletii type strain. This sequence will provide essential information for future taxonomic and comparative genome studies of these mycobacteria.

Complete Genome Sequence of Mycobacterium marinum ATCC 927 T , Obtained Using Nanopore and Illumina Sequencing Technologies

“…The library was loaded on a SpotON Mk I (R9.4) flow cell and sequenced using MinKNOW version 1.7.14, and raw sequence data (FAST5 format) were base-called using Albacore Sequencing Pipeline version 2.0.2 software. The reads were de novo assembled into two contigs (6,456,544 bp and 173,286 bp) with Canu version 1.5 ( 15 , 16 , 17 ), and the assembled genome was circularized by manually trimming the repeated sequences. Illumina paired-end (2 × 150-bp) reads (266,781,451 reads) were obtained with the MiniSeq system (Illumina, San Diego, CA, USA) and mapped to the assembly using the Burrows-Wheeler aligner ( 15 , 17 , 18 ) for sequence and assembly error correction with Pilon ( 15 , 17 , 19 ).…”

“…Orthologous gene clusters were identified using Cd-hit ( 21 ). Average nucleotide identity (ANI) was calculated by JSpeciesWS ( 15 , 17 , 22 ).…”

Mycobacterium abscessus complex: A Review of Recent Developments in an Emerging Pathogen

“…Transmission updates suggest that MABC is transmissible between CF patients in the hospital setting, including the transmission of macrolide-resistant isolates (Köser et al, 2014;Bryant et al, 9877). The finding that resistant strains might be transmissible has created a shift in our knowledge of MABC transmission (Ravnholt et al, 2018;Yoshida et al, 2018), and raised concerns about the clinical impact associated with global propagation of these strains.…”

Molecular epidemiological characteristics of Mycobacterium abscessus complex in non-cystic fibrosis patients in Japan and Taiwan

“…massiliense JCM15300 (47), and M. abscessus subsp. bolletii BD (48). For phylogenetic analyses, we first performed pairwise genome alignment between ATCC19977 and one of the clinical isolates using the MUMmer package (49) to identify conserved genomic regions among these isolates and the SNP sites located within these regions.…”