Complete Genome Sequence of an Avian-Origin H3N2 Canine Influenza Virus Isolated from Dogs in South Korea

Abstract

An avian-origin Korean H3N2 canine influenza virus (CIV) strain, designated A/canine/Korea/01/2007 (H3N2), was isolated from nasal swabs of pet dogs exhibiting severe respiratory syndrome in 2007. In the present study, we report the first complete genome sequence containing 3′ and 5′ noncoding regions (NCRs) of H3N2 CIV, which will provide important insights into the molecular basis of pathogenesis, transmission, and evolution of CIV.

Complete Genome Sequence of a Mammalian Species-Infectious and -Pathogenic H6N5 Avian Influenza Virus without Evidence of Adaptation

“…They encode 12 viral proteins with amino acid lengths as follows: PB2, 759; PB1, 757; N40 (an N-terminally truncated and functionally distinct variant of PB1) (17) nt at the 3= and 5= NCRs, respectively) in the different genome segments, but the terminal 12 (3=UCGYUUUCGUCC-) and 13 (-GGAACAAAGAUGA5=) nt of the 3= and 5= ends, respectively, were highly conserved among all genome segments, which is consistent with results of previous studies (1,8,11). Furthermore, a uridine-rich region (5 to 6 U's), which serves as the polyadenylation site (10,12), was observed from positions 15 through 16 at the 5= end of each segment.…”

Complete Genome Sequence of a Canine-Origin H3N2 Feline Influenza Virus Isolated from Domestic Cats in South Korea

“…While the lengths of the 3′ and 5′ NCRs of the viral RNA of A/feline/Korea/01/2010 (H3N2) were variable (19 to 45 and 20 to 58 nt at the 3′ and 5′ NCRs, respectively) in the different genome segments, the terminal 12 and 13 nt of the 3′ and 5′ ends, respectively, were highly conserved (3′-UCGYUUUCGUCC- and -GGAACAAAGAUGA-5′) among all eight genome segments, which is consistent with previous studies (13, 14, 19, 20). Surprisingly, 1 nt was changed between the start codon (UAC) and the conserved region (UCGYUUUCGUCC) in the 3′ NCR of Seg-1 and also in Seg-6 of A/feline/Korea/01/2010 (H3N2) compared to A/canine/Korea/01/2007 (H3N2).…”

“…The complete genome of A/feline/Korea/01/2010 (H3N2) is 13,629 nucleotides (nt) long, a length identical to that of the avian-origin Korean H3N2 canine influenza virus, A/canine/Korea/01/2007 (H3N2) (13); segments 1 (Seg-1) through 8 (Seg-8) are 2,341, 2,341, 2,233, 1,765, 1,565, 1,467, 1,027, and 890 nt, respectively. They encode 12 viral proteins with amino acid lengths as follows: PB2, 759; PB1, 757; N40 (18), 718; PB1-F2, 90; PA, 716; HA, 566; NP, 498; NA, 469; M1, 252; M2, 97; NS1, 230; and NS2 (nuclear export protein [NEP]), 121.…”

Isolation and genetic characterization of naturally NS-truncated H3N8 equine influenza virus in South Korea

“…However, in East Asia, i.e. South Korea and China, H3N2 CIV is prevalent; this CIV originated from avian influenza A virus[28][29][30] and transmission of H3N8 EIV to dogs has not yet been reported. Recently, H3N1 CIV, representing reassortment between pandemic H1N1 virus and the H3N2 CIV, was isolated from a single pet dog…”