Complete Genome Sequence of Klebsiella pneumoniae Phages SopranoGao, MezzoGao, and AltoGao

Gao, Sarah; Linden, Sara B.; Nelson, Daniel

doi:10.1128/genomea.01009-17

Cited by 7 publications

(3 citation statements)

References 4 publications

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“…The φBO1E phage belongs to the Podoviridae family and is a member of the recently described Drulisvirus genus [ 27 ]. Even if the information on the infection specificity of Drulisvirus phages is currently available in a limited number of cases, i.e., KpV41, KpV48, KpV71, KpV74, KpV475 [ 28 ], myPSH1235 [ 29 ], πVLC1-4 [ 30 ] and vB_KpnP_IME337 [ 31 ], for most of these phages a perfect correlation between the bacterial cps type and phage susceptibility has been demonstrated [ 10 , 28 , 30 , 31 ] while this information is lacking or a broader specificity has been determined in a limited number of studies [ 32 , 33 , 34 , 35 , 36 , 37 , 38 ] ( Table S2 ). This is in line with our results and, together with the observation that these phages possess a pectate lyase domain in their terminal fibers, suggests that this genus is characterized by the ability of targeting bacterial polysaccharides for the early infection steps.…”

Section: Discussionmentioning

confidence: 99%

Phage Resistance Is Associated with Decreased Virulence in KPC-Producing Klebsiella pneumoniae of the Clonal Group 258 Clade II Lineage

et al. 2021

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Phage therapy is now reconsidered with interest in the treatment of bacterial infections. A major piece of information for this application is the definition of the molecular targets exploited by phages to infect bacteria. Here, the genetic basis of resistance to the lytic phage φBO1E by its susceptible host Klebsiella pneumoniae KKBO-1 has been investigated. KKBO-1 phage-resistant mutants were obtained by infection at high multiplicity. One mutant, designated BO-FR-1, was selected for subsequent experiments, including virulence assessment in a Galleria mellonella infection model and characterization by whole-genome sequencing. Infection with BO-FR-1 was associated with a significantly lower mortality when compared to that of the parental strain. The BO-FR-1 genome differed from KKBO-1 by a single nonsense mutation into the wbaP gene, which encodes a glycosyltransferase involved in the first step of the biosynthesis of the capsular polysaccharide (CPS). Phage susceptibility was restored when BO-FR-1 was complemented with the constitutive wbaP gene. Our results demonstrated that φBO1E infects KKBO-1 targeting the bacterial CPS. Interestingly, BO-FR-1 was less virulent than the parental strain, suggesting that in the context of the interplay among phage, bacterial pathogen and host, the emergence of phage resistance may be beneficial for the host.

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Section: Discussionmentioning

confidence: 99%

Phage Resistance Is Associated with Decreased Virulence in KPC-Producing Klebsiella pneumoniae of the Clonal Group 258 Clade II Lineage

et al. 2021

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“…In light of the problem of the development and spread of antibiotic resistant bacteria, phages have been investigated as alternative antimicrobial agents to treat bacterial infections in humans 12 – 14 , and several studies have isolated and characterize phages specific for K. pneumoniae 15 – 18 . For example K. pneumoniae phage KN2 19 , NTUH-K2044-K1-1 20 , phage KP36 21 have exhibited robust activities with high target specificity, possibly as a consequence of their depolymerases and polysaccharide degrading enzymes which can degrade polysaccharides such as capsular polysaccharide (CPS), extracellular polymeric substance (EPS) and biofilm 22 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of extended-spectrum-β-lactamase producing Klebsiella pneumoniae phage KP1801 and evaluation of therapeutic efficacy in vitro and in vivo

Wintachai

Naknaen

Thammaphet

et al. 2020

Sci Rep

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Extended spectrum β lactamase-producing Klebsiella pneumoniae (ESBL-KP) is being reported with high morbidity and mortality rates and is considered as the highest priority for new antimicrobial strategies. To develop an alternative antimicrobial agent, phage KP1801 with broad lytic activity was isolated. The genome of phage KP1801 was double stranded DNA of 49,835 base pairs, with a GC content of 50.26%. There were 75 putative open reading frames. Phage KP1801 was classified as being in the order Caudovirales , belonging to the Siphoviridae family. About 323 proteins were detected by shotgun proteome analysis. The phage inhibited biofilm formation and reduced pre-formed biofilm in a dose dependent manner. Scanning electron microscopic studies demonstrated a membrane damage of bacterial cells treated with phage, resulting in cell death. Prophylactic and therapeutic efficacies of the phage were evaluated in Galleria mellonella . Administration of ESBL-KP infection with phage significantly improved the survival of G. mellonella . The number of intracellular bacteria in larvae showed a significant decrease compared with untreated control while the number of phage increased. These studies suggested that phage KP1801 has the potential for development as an alternative for antibiotics and biocontrol agents against ESBL-KP infection.

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“…By using progressiveMauve 2.4.0, we found that Skenny shares high nucleotide identity with various T1-like phages, including 96% and 98% identity with K. pneumoniae phages KPN N141 (GenBank accession number MF415412) and MezzoGao (GenBank accession number MF612072), respectively (21). There are 78 genes predicted to encode proteins for Skenny, but no tRNAs were detected.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Klebsiella pneumoniae Siphophage Skenny

Gramer

Kenny

Newkirk

et al. 2019

Microbiol Resour Announc

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Complete Genome Sequence of Klebsiella pneumoniae Phages SopranoGao, MezzoGao, and AltoGao

Abstract: The Klebsiella pneumoniae phages SopranoGao, MezzoGao, and AltoGao were isolated from the Seneca Wastewater Treatment Plant in Germantown, MD. The following reports the complete genome sequence of these bacteriophages and describes their major features.

Cited by 7 publications

References 4 publications

Phage Resistance Is Associated with Decreased Virulence in KPC-Producing Klebsiella pneumoniae of the Clonal Group 258 Clade II Lineage

Phage Resistance Is Associated with Decreased Virulence in KPC-Producing Klebsiella pneumoniae of the Clonal Group 258 Clade II Lineage

Characterization of extended-spectrum-β-lactamase producing Klebsiella pneumoniae phage KP1801 and evaluation of therapeutic efficacy in vitro and in vivo

Complete Genome Sequence of Klebsiella pneumoniae Siphophage Skenny

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