Complete Genome Sequence of Mycoplasma bovis Strain KG4397, Isolated from Cattle in Japan

Abstract

Mycoplasma bovis is a major bacterial pathogen that causes pneumonia, mastitis, and arthritis in cattle. In this study, we performed whole-genome sequencing of an M. bovis strain isolated in Japan for the first time and announce the complete genome sequence of strain KG4397, which caused respiratory diseases in cattle in 2012.

Comparison of Two Multilocus Sequence Typing Schemes for Mycoplasma bovis and Revision of the PubMLST Reference Method

“…Isolates in this group were found in cattle, bison, and a white-tailed deer originating from geographically diverse regions that include North America, Europe, the Middle East, and Australia. The combination of alleles defining 12 of the partial profiles is novel compared to the 185 STs so far reported here or elsewhere (10,13,16,17,32,33) or submitted to the PubMLST Mycoplasma. bovis Locus/Sequence Definitions Database (Table 2).…”

“…It now appears that clones missing adh-1 may have first arisen around that time and that their prevalence is growing worldwide. Considering all typing data currently available, reported either here or elsewhere (10,13,16,17,32,33) or submitted to the PubMLST Mycoplasma bovis Isolates Database, the adh-1 locus is absent in only 1.4% (5/349) of isolates obtained in 2010 or prior, compared to 14.2% (86/606) of isolates originating after that time. No isolates missing that locus were found among the 137 included in the study describing the development of the PubMLST scheme ( 13), perhaps because the majority (92/137, or 67.2%) originated in 2010 or earlier.…”

“…All isolates were assigned allelic profiles and STs according to the scheme currently used as the PubMLST reference method, based on partial sequences ranging in size from 306 to 570 bp, from the seven housekeeping genes adh-1, gltX, gpsA, gyrB, pta-2, tdk, and tkt (13; https://pubmlst.org/ mbovis/). STs for 101 cattle isolates, 31 bison isolates, and the white-tailed deer isolate were reported in prior studies (10,13,17; indicated by bold font in Table S1). STs for all remaining isolates were assigned on the basis of output from either the PubMLST automated sequence tag-scanning function (for data derived from genome assemblies) or from sequence queries of the PubMLST Mycoplasma bovis Locus/ Sequence Definitions Database (https://pubmlst.org/bigsdb?dbϭpubmlst_mbovis_seqdef) for data obtained from sequencing PCR amplicons.…”

Complete Genome Sequences of 16 Mycoplasma bovis Isolates from Canadian Bison and Cattle

“…One theory suggests that the relatively recent appearance of M. bovis as a pathogen in bison is due to the emergence of unique, newly evolved genotypes with an expanded host range or heightened virulence. The complete genome sequences for 11 isolates from cattle have been reported (6)(7)(8)(9)(10)(11) , and 500 IU/ml penicillin G [Sigma]), followed by plating onto selective PPLO agar. Axenic seed stocks generated from single, wellisolated colonies grown in selective PPLO broth were confirmed to be M. bovis using a diagnostic PCR (12).…”