Complete germination of papaya (Carica papaya L. cv. `Maradol Roja´) somatic embryos using temporary immersion system type RITA® and phloroglucinol in semi-solid culture medium

Pérez, Laisyn Posada; Montesinos, Yenny Padrón; Guerra, Diosdada Gálvez; Daniels, Dion; Gómez-Kosky, Rafael

doi:10.1007/s11627-017-9842-5

Cited by 20 publications

(12 citation statements)

References 33 publications

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“…Our findings were confirmed by Posada-Perez et al [22] who reported that germination rate of somatic embryos of papaya (Carica papaya L.) was low at the density of 300 mg FWt since inadequate amount of water, sucrose and nutrients available in the culture medium and by Romero et al [23] who stated that reducing plant density was because of reducing competition between protocorms of orchid Chloraea gavilu Lindl.. Photosynthetic activity as chlorophyll a, chlorophyll b and total chlorophyll content which was an indicator of seedling health and vitality, was also higher at a distance of '1.5x1.5' cm. At other distances, the seedlings seemed to slow down their photosynthetic activity due to stress.…”

Section: Resultssupporting

confidence: 92%

The Effect of Inter-Plantal Competition on in Vitro Seed Germination and Seedling Growth in Flax (Linum Usitatissimum L.)

Beyaz¹,

Yıldız²

2019

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology

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This study was carried out to investigate the relationship between in vitro competition and stress, and their effects on seedling growth of three flax (Linum usitatissimum) cultivars ('Madaras', 'Clarck', and '1886 Sel.'). Competition among plants was achieved by changing the distances they sown. Four different distances were used as '0.5x0.5', '1.0x1.0', '1.5x1.5' and '2.0x2.0' cm. The results showed that encouraging seedlings for competition by decreasing the distances among seeds sown per Magenta vessel elevated chlorophyll contents (chl. a, b and total chl.), seedling height and root length significantly up to a threshold from where stress initiated and significant decreases in all parameters were observed. In term of chlorophyll contents (chl. a, b and total chl.), seedling height and root length, the highest results (1540.1, 581.9, and 1253.7 µg/g tissue, 4.54 and 7.77 cm, respectively) were obtained from '1.5x1.5' cm distance in cv. 'Madaras'. For cv. 'Clarck', the highest values in chlorophyll contents (chl. a, b and total chl.), seedling height and root length were recorded as 1860.6, 652.6, and 1429.0 µg/g tissue, 4.58 and 6.79 cm, respectively in '1.5x1.5' cm distance application. The highest results with respect to chlorophyll contents (chl. a, b and total chl.), seedling height and root length were aigain recorded at '1.5x1.5' cm distance application in cv. '1886 Sel.' as 1782.6, 552.0, and 1138.7 µg/g tissue, 4.62 and 6.45 cm, respectively. As a consequence, the application of '1.5x1.5' cm distance seems to best for all cultivars. This study showed that competition among in vitro-grown seedlings which can be the source of explants may be used to increase the success of tissue culture studies.

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Section: Resultssupporting

confidence: 92%

The Effect of Inter-Plantal Competition on in Vitro Seed Germination and Seedling Growth in Flax (Linum Usitatissimum L.)

Beyaz¹,

Yıldız²

2019

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology

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“…The phenomenon of callus formation at the base of the shoots is very common in papayas, and it inhibits the complete germination of somatic embryos. Indeed, it has been previously reported in babaco (Vega de Rojas and Kitto, ) and C. papaya (Ascencio‐Cabral et al., ; Sekeli et al., ; Dhekney et al., ; Posada‐Pérez et al., ). Recently, Posada‐Pérez et al.…”

Section: Discussionmentioning

confidence: 80%

“…Recently, Posada‐Pérez et al. () circumvented this problem in the germination of C. papaya somatic embryos using two strategies: first by adding 475.8 μM phloroglucinol in semisolid medium, and second by cultivating the somatic embryos in liquid medium in a RITA temporary immersion system combining 0.02 μM 6‐BAP and 2.90 μM gibberellic acid. In the future, more research is needed in V. pubescens to determine if somatic embryogenesis is occurring and to increase regeneration efficiency.…”

Section: Discussionmentioning

confidence: 99%

“…The Caricaceae family contains a number of important plant species with very high economic and environmental value (Scheldeman et al., ; Ming et al., ). Many studies are now being conducted on the genetic improvement and conservation (Carrasco et al., , ; Carvalho and Renner, ; de Oliveira et al., ; Brown et al., ), genomics (Ming et al., , ), and plant propagation (Jordan, ; Dhekney et al., ; Posada‐Pérez et al., ) of many species in this family. The conditions for plant regeneration reported here can be very helpful for some of these projects, as well as for new research line developments.…”

Section: Discussionmentioning

confidence: 99%

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Regeneration of highland papaya (Vasconcellea pubescens) from anther culture

Chong-Pérez¹,

Carrasco

Silva

et al. 2018

Appl Plant Sci

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Premise of the Study Vasconcellea pubescens is an important Caricaceae species cultivated in several countries of South America. The objective of this study was to investigate different media compositions and plant growth regulators to induce plant regeneration.MethodsAnthers were cultured in Murashige and Skoog medium with varying concentrations of naphthalene acetic acid (NAA) and 2,4‐dichlorophenoxyacetic acid (2,4‐D) plus a cytokinin (N‐(2‐chloro‐4‐pyridyl)‐N′‐phenylurea). The effect of the basal medium supplemented with auxins and cytokinins on shoot regeneration from the induced calli was also evaluated. Addition of maltose to the basal medium was also tested.ResultsThe combination of 0.54 μM NAA and 22.66 μM 2,4‐D induced the highest rate of calli formation. Regeneration via organogenesis was obtained in Murashige and Skoog and Woody Plant Medium supplemented with maltose and containing 8.88 μM 6‐benzylaminopurine, 5.71 μM indoleacetic acid, and 2.28 μM zeatin.DiscussionThe plant regeneration protocol reported here permits the development of haploid and double haploid plants that can be useful for propagation purposes, allow a better molecular understanding of the species, and facilitate the production of new cultivars.

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“…In other plants as rubber tree, kalopanax, papaya, guava, and cacao improvements in embryo germination and plant conversion were observed [87][88][89][90][91], although its commercial application did not reach the level of success of coffee.…”

Section: Somatic Embryos Cultured By Temporary and Continuous Immersionmentioning

confidence: 98%

Use of bioreactor systems in the propagation of forest trees

Vidal

Sánchez

2019

Engineering in Life Sciences

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Plant biotechnology can be used to conserve the germplasm of natural forests, and to increase the productivity and sustainability of plantations. Both goals imply working with mature trees, which are often recalcitrant to micropropagation. Conventional in vitro culture uses closed containers and gelled medium with sugar supplementation. Bioreactor culture uses liquid medium and usually incorporates aeration. The increased absorption of nutrients via the liquid medium together with the renewal of the air inside the bioreactors may improve the physiological state of the explants. In this review, we will explore the feasibility of using bioreactors to overcome the recalcitrance of many trees to micropropagation and/or to decrease the cost of large‐scale propagation. We will focus on the recent use of bioreactors during the multiplication, rooting (plant conversion in the case of somatic embryos), and acclimation stages of the micropropagation of axillary shoots and somatic embryos of forest trees (including some shrubs of commercial interest), in both temporary and continuous immersion systems. We will discuss the advantages and the main obstacles limiting the widespread implementation of bioreactor systems in woody plant culture, considering published scientific reports and contributions from the business sector.

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Complete germination of papaya (Carica papaya L. cv. `Maradol Roja´) somatic embryos using temporary immersion system type RITA® and phloroglucinol in semi-solid culture medium

Cited by 20 publications

References 33 publications

The Effect of Inter-Plantal Competition on in Vitro Seed Germination and Seedling Growth in Flax (Linum Usitatissimum L.)

The Effect of Inter-Plantal Competition on in Vitro Seed Germination and Seedling Growth in Flax (Linum Usitatissimum L.)

Regeneration of highland papaya (Vasconcellea pubescens) from anther culture

Use of bioreactor systems in the propagation of forest trees

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