Complete mitochondrial genome of two Thitarodes species (Lepidoptera, Hepialidae), the host moths of Ophiocordyceps sinensis and phylogenetic implications

Zhang, Min; Gao, Zhen; Yin, Jia; Zhang, Tingting; Zhang, Xueyao; Yuan, Dong‐Wei; Li, Tao; Zhong, Yang; Ma, Enbo; Ren, Zhumei

doi:10.1016/j.ijbiomac.2019.08.182

Cited by 8 publications

(14 citation statements)

References 51 publications

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“…The mitochondrion, a circle organelle exists in almost eukaryotes species cell, which usually provides a source of chemical energy through oxidative phosphorylation [ 11 ]. The mitochondrion exhibits many advantages, such as their evolutionary rate being faster than nuclear DNA, the characteristics of conservation gene functions, their high copy number and AT content [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Mitochondrial Genome Features among Four Clonostachys Species and Insight into Their Systematic Positions in the Order Hypocreales

Zhao

Zhu

Tang

et al. 2021

IJMS

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The mycoparasite fungi of Clonostachys have contributed to the biological control of plant fungal disease and nematodes. The Clonostachys fungi strains were isolated from Ophiocordyceps highlandensis, Ophiocordycepsnigrolla and soil, which identified as Clonostachyscompactiuscula, Clonostachysrogersoniana, Clonostachyssolani and Clonostachys sp. To explore the evolutionary relationship between the mentioned species, the mitochondrial genomes of four Clonostachys species were sequenced and assembled. The four mitogenomes consisted of complete circular DNA molecules, with the total sizes ranging from 27,410 bp to 42,075 bp. The GC contents, GC skews and AT skews of the mitogenomes varied considerably. Mitogenomic synteny analysis indicated that these mitogenomes underwent gene rearrangements. Among the 15 protein-coding genes within the mitogenomes, the nad4L gene exhibited the least genetic distance, demonstrating a high degree of conservation. The selection pressure analysis of these 15 PCGs were all below 1, indicating that PCGs were subject to purifying selection. Based on protein-coding gene calculation of the significantly supported topologies, the four Clonostachys species were divided into a group in the phylogenetic tree. The results supplemented the database of mitogenomes in Hypocreales order, which might be a useful research tool to conduct a phylogenetic analysis of Clonostachys. Additionally, the suitable molecular marker was significant to study phylogenetic relationships in the Bionectriaceae family.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Mitochondrial Genome Features among Four Clonostachys Species and Insight into Their Systematic Positions in the Order Hypocreales

Zhao

Zhu

Tang

et al. 2021

IJMS

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“…Long-distance dispersal of host insects seems very limited due to the short lifespan of the adult and the heavy abdomens of females that carry eggs; thus most shared genotypes are between strains from adjacent geographic regions [14]. Given the complex and harsh ecological environment in the Tibetan Plateau, most Hepialidae species have a narrow-area distribution type [21]. Of ninety-one named Hepialidae insects spanning thirteen genera reported to be related to host insects of the O. sinensis fungus, fifty-seven are considered potential host species of the fungus and are distributed throughout the Tibetan Plateau [17].…”

Section: Introductionmentioning

confidence: 99%

“…Mitochondrial genome sequences have been widely used as molecular markers for diverse evolutionary analyses because of their unique features, including coding content conservation, maternal inheritance and rapid evolution [32]. Insect mitochondrial genomes are usually small closed-circular molecules (15-20 kb) containing 13 proteincoding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and a large non-coding element termed the A + T-rich or control region [21,33,34]. The mitochondrial gene order also provides important evidence for establishing genome evolutionary relationships [34,35].…”

Section: Introductionmentioning

confidence: 99%

“…Due to the improved sequencing technology, insect mitochondrial genomes have been heavily sequenced in recent years. Eight Thitarodes/Hepialus mitochondrial genomes are reported, including Thitarodes renzhiensis (accession number HM744694; size 16,173 bp), Thitarodes yunnanensis (former Ahamus yunnanensis) (accession number HM744695; size 15,816 bp) [21,36], Thitarodes pui (accession numbers KF908880 and MK599283; sizes 15,064 bp and 15,928 bp) [21,37], Hepialus xiaojinensis (accession number KT834973; size 15,397 bp) [38], Hepialus gonggaensis (accession number KP718817; size 15,940 bp) [39], Thitarodes sejilaensis (accession number KU053201; size 15,290 bp;) [40], Thitarodes sp. (accession number KX527574; size 16,280 bp) [41] and Thitarodes damxungensis (accession number MK648145; size 15,362 bp) [21].…”

Section: Introductionmentioning

confidence: 99%

“…Eight Thitarodes/Hepialus mitochondrial genomes are reported, including Thitarodes renzhiensis (accession number HM744694; size 16,173 bp), Thitarodes yunnanensis (former Ahamus yunnanensis) (accession number HM744695; size 15,816 bp) [21,36], Thitarodes pui (accession numbers KF908880 and MK599283; sizes 15,064 bp and 15,928 bp) [21,37], Hepialus xiaojinensis (accession number KT834973; size 15,397 bp) [38], Hepialus gonggaensis (accession number KP718817; size 15,940 bp) [39], Thitarodes sejilaensis (accession number KU053201; size 15,290 bp;) [40], Thitarodes sp. (accession number KX527574; size 16,280 bp) [41] and Thitarodes damxungensis (accession number MK648145; size 15,362 bp) [21]. With respect to a total of 57 recognizable potential host species of the fungus, the information of the mitochondrial genomes of existing ghost moths is still very limited, and no reports are available on the mitochondrial genomes from the hybrids.…”

Section: Introductionmentioning

confidence: 99%

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Interspecific Hybridization and Complete Mitochondrial Genome Analysis of Two Ghost Moth Species

Cao

et al. 2021

Insects

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The Chinese cordyceps, a parasitic Ophiocordyceps sinensis fungus–Thitarodes/Hepialus larva complex, is a valuable biological resource endemic to the Tibetan Plateau. Protection of the Plateau environment and huge market demand make it necessary to culture this complex in an artificial system. A method for the large-scale artificial rearing of the Thitarodes/Hepialus insect host has been established. However, the deterioration of the insect rearing population and low mummification of the infected larvae by the fungus constrain effective commercial cultivation. Hybridization of Thitarodes/Hepialus populations may be needed to overcome this problem. The species T. shambalaensis (GG♂ × GG♀) and an undescribed Thitarodes species (SD♂ × SD♀) were inbred or hybridized to evaluate the biological parameters, larval sensitivity to the fungal infection and mitochondrial genomes of the resulting populations. The two parental Thitarodes species exhibited significant differences in adult fresh weights and body lengths but not in pupal emergence rates. Hybridization of T. shambalaensis and Thitarodes sp. allowed producing a new generation. The SD♂ × GG♀ population showed a higher population trend index than the SD♂ × SD♀ population, implying increased population growth compared with the male parent. The sensitivity of the inbred larval populations to four fungal isolates of O. sinensis also differed. This provides possibilities to create Thitarodes/Hepialus populations with increased growth potential for the improved artificial production of the insect hosts. The mitochondrial genomes of GG♂ × GG♀, SD♂ × SD♀ and SD♂ × GG♀ were 15,612 bp, 15,389 bp and 15,496 bp in length, with an A + T content of 80.92%, 82.35% and 80.87%, respectively. The A + T-rich region contains 787 bp with two 114 bp repetitive sequences, 554 bp without repetitive sequences and 673 bp without repetitive sequences in GG♂ × GG♀, SD♂ × SD♀ and SD♂ × GG♀, respectively. The hybrid population (SD♂ × GG♀) was located in the same clade with GG♂ × GG♀, based on the phylogenetic tree constructed by 13 PCGs, implying the maternal inheritance of mitochondrial DNA.

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The phylogeny and divergence time of Ophiocordyceps sinensis and its host insects based on elongation factor 1 alpha

Zhu

et al. 2023

Arch Microbiol

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Complete mitochondrial genome of two Thitarodes species (Lepidoptera, Hepialidae), the host moths of Ophiocordyceps sinensis and phylogenetic implications

Cited by 8 publications

References 51 publications

Comparative Analysis of Mitochondrial Genome Features among Four Clonostachys Species and Insight into Their Systematic Positions in the Order Hypocreales

Comparative Analysis of Mitochondrial Genome Features among Four Clonostachys Species and Insight into Their Systematic Positions in the Order Hypocreales

Interspecific Hybridization and Complete Mitochondrial Genome Analysis of Two Ghost Moth Species

The phylogeny and divergence time of Ophiocordyceps sinensis and its host insects based on elongation factor 1 alpha

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