Composite Hydrogel of Methacrylated Hyaluronic Acid and Fragmented Polycaprolactone Nanofiber for Osteogenic Differentiation of Adipose-Derived Stem Cells

Abstract

Composite hydrogels with electrospun nanofibers (NFs) have recently been used to mimic the native extracellular matrix. In this study, composite hydrogels of methacrylated hyaluronic acid containing fragmented polycaprolactone NFs were used for bone tissue engineering. The composite (NF/hydrogel) was crosslinked under ultraviolet (UV) light. The incorporation of fragmented polycaprolactone NFs increased the compression modulus from 1762.5 to 3122.5 Pa. Subsequently, adipose-derived stem cells incorporated into the composite hydrogel exhibited a more stretched and elongated morphology and osteogenic differentiation in the absence of external factors. The mRNA expressions of osteogenic biomarkers, including collagen 1 (Col1), alkaline phosphatase, and runt-related transcription factor 2, were 3–5-fold higher in the composite hydrogel than in the hydrogel alone. In addition, results of the protein expression of Col1 and alizarin red staining confirmed osteogenic differentiation. These findings suggest that our composite hydrogel provides a suitable microenvironment for bone tissue engineering.

Effect of Polymeric Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem/Progenitor Cells: Concise Review

“…The biomarkers collage type 1, ALP, and Runx2 were significantly expressed in the hydrogels containing nanofibers. In addition, the results of alizarin red staining confirmed osteogenic differentiation [178].…”

Aptamer/Hydroxyapatite-Functionalized Titanium Substrate Promotes Implant Osseointegration via Recruiting Mesenchymal Stem Cells

“…Hydroxyapatite topology can guide the directional arrangement of MSCs on the surface of the material through contact and control the directional deposition of cells on the surface, thereby promoting the osteogenic differentiation of MSCs. , This line of evidence suggests that hydroxyapatite is beneficial to the enrichment of various endogenous bone growth factors, inducing the differentiation of MSCs into bone precursor cells. As a basic component of ECM, hyaluronic acid can regulate the biological behavior of cells by binding to specific receptors on target cells, stimulate the osteogenic signaling pathway, and promote the osteogenic differentiation of MSCs. , Photo-cross-linked methacrylic acid/hyaluronic acid/polycaprolactone (PCL) fibrous hydrogels, the significant increase in the expression of osteogenic markers collage type I, ALP, and Runx-2, and the mineralization of extracellular matrix indicate that HA can promote the osteogenic differentiation of MSCs. , …”

“…As a basic component of ECM, hyaluronic acid can regulate the biological behavior of cells by binding to specific receptors on target cells, stimulate the osteogenic signaling pathway, and promote the osteogenic differentiation of MSCs. 49,50 Photocross-linked methacrylic acid/hyaluronic acid/polycaprolactone (PCL) fibrous hydrogels, 51 the significant increase in the expression of osteogenic markers collage type I, ALP, and Runx-2, and the mineralization of extracellular matrix indicate that HA can promote the osteogenic differentiation of MSCs. 52,53 In general, Ti/HAp/OHA-Apt recruited MSCs to the material site by releasing Apt19s, and MSCs underwent osteogenic differentiation under the combined action of hyaluronic acid and HAp and finally improves osseointegration of the implants.…”

Dendritic cell-mimicking scaffolds for ex vivo T cell expansion

“…Noteworthy, this system is a modular platform that can present diverse cues specific to target cells or diseases. For example, electrospun fiber-based scaffolds can be tailored to tune their size, dimension, composition and configuration [ [48] , [49] , [50] ]. The cell membrane can be engineered to tune affinity, specificity, surface cue partitioning, or release kinetics of cytokine payloads [ 51 , 52 ].…”