Composition of the bovine uterine proteome is associated with stage of cycle and concentration of systemic progesterone

Faulkner, Simon; Elia, Giuliano; O'Boyle, Padraic; Dünn, Michael J.; Morris, Deborah

doi:10.1002/pmic.201300204

Cited by 48 publications

(43 citation statements)

References 117 publications

(137 reference statements)

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“…The stimulatory effect of P4 on trophoblast elongation, although indirect via the endometrium, is unequivocal. This effect is a result of downstream effects of P4-induced changes in gene expression in cells of the endometrium (Satterfield et al 2006, 2012b resulting in changes in the composition of ULF to which the developing embryo is exposed (Faulkner et al 2013) (Fig. 1).…”

Section: Conceptus Size and Progesterone Studiesmentioning

confidence: 99%

Interferon-tau and fertility in ruminants

Forde¹,

Lonergan²

2017

Reproduction

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Establishment of pregnancy in domestic ruminants includes pregnancy recognition signalling by the conceptus, implantation and placentation. Despite the high fertilisation success rate in ruminants, a significant amount of embryo loss occurs, primarily during early gestation. Interferon-tau (IFNT), a type I interferon that is exclusively secreted by the cells of the trophectoderm of the ruminant conceptus, has been recognised as the primary agent for maternal recognition of pregnancy in ruminants. It produces its antiluteolytic effect on the corpus luteum by inhibiting the expression of oxytocin receptors in the uterine epithelial cells, which prevents pulsatile, luteolytic secretion of prostaglandin F2α by the uterine endometrium. While the importance of IFNT in maternal recognition of pregnancy and prevention of luteolysis in ruminants is unequivocal, important questions, for example, relating to the threshold level of IFNT required for pregnancy maintenance, remain unanswered. This paper reviews data linking IFNT with measures of fertility in ruminants. Reproduction (2017) 154 F33-F43

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Section: Conceptus Size and Progesterone Studiesmentioning

confidence: 99%

Interferon-tau and fertility in ruminants

Forde¹,

Lonergan²

2017

Reproduction

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“…To maintain the uterine functions, different proteins secreted by the endometrium act on sperm viability (Martus, Verhage, Mavrogianis, & Thibodeaux, ), embryo metabolism, implantation and maintenance of the corpus luteum (Ziecik et al, ). For this reason, the study of such proteins at specific times (different stages of the oestrous cycle or during pregnancy) has relevance in the reproduction of females, given that their quantity and type change according to the physiological status (Faulkner, Elia, & O'Boyle, P., Dunn, M., & Morris, D., ) and may interfere in the periovulatory phase, which is the period normally used for the transfer of embryos in cloning biotechniques (Martins et al , ). In recent years, studies have used methods in proteomics to investigate the uterine molecular dynamics as related to uterus physiology in the caprine species (Koch et al, ).…”

Section: Introductionmentioning

confidence: 99%

Proteome of the periovulatory oviduct and uterus of goats as related to nutritional balance

Fernandes

Rodríguez-Villamil

Vasconcelos

et al. 2018

Reprod Domestic Animals

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This study was conducted to evaluate the effects of different feeding levels on the proteome of oviduct and uterus tissues of hormonally stimulated goats during the periovulatory period. Forty goats were separated into four different diet groups: Diet 1.0 M (n = 11), Diet 1.3 M (n = 10), Diet 1.6 M (n = 9), Diet 1.9 M (n = 10), fed with 1.0, 1.3, 1.6 and 1.9 times live weight maintenance, respectively. After four weeks of treatment, six hormonally stimulated females per treatment group were randomly selected for collection of uterine and the oviduct tissue samples. Samples were collected after animals were slaughtered in a commercial unit. Feeding goats with 1.3 to 1.9 times more nutrients than a control group directly influenced the proteome of the oviduct and uterus, altering the expression of proteins that participate in biological processes such as apoptosis, antioxidant, and immunological activities. These events are crucial for fertilization and early embryonic survival. Expression of oviduct proteins such as Tubulin Beta 2B, Transferrin and Disulphide-isomerase A3 increased in the 1.9 M group in relation to the other feeding levels. Disulphide-isomerase A4 showed higher expression in the 1.0 M group compared to diets with higher energetic levels. As energy intake increased in the diets, there was higher expression of Alpha-1-antitrypsin and downregulation of Profilin-1 in the uterus of the goats. In conclusion, this study showed that specific proteins of the goat oviduct and uterus expressed during the periovulatory period are modified as the result of nutritional balance.

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“…There is evidence that protein synthesis remains under the control of steroid hormones and alterations in protein profiles during pregnancy can be related to the changes in gestational hormonal profile. Faulkner et al confirmed this relationship in cyclic cows and Balestrieri et al in pregnant cows.…”

Section: Introductionmentioning

confidence: 74%

Identification of protein patterns in bovine placenta at early‐mid pregnancy – Pilot studies

Ner‐Kluza

Wawrzykowski

Franczyk

et al. 2019

Rapid Comm Mass Spectrometry

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Rationale Placenta is a crucial tissue for an appropriate development of the fetus and the course of pregnancy. Its composition and structure change dynamically along pregnancy but the full pattern of these changes is not fully described in cows yet. The aim of the present study was to detect qualitative and quantitative protein profiles of bovine placenta during early‐mid pregnancy at the time of placental formation. Methods Placental tissues from healthy cows (n = 3) in early pregnancy (3–5 months) were collected at the slaughterhouse. Maternal and fetal parts were manually divided prior to homogenization. Further analysis was done in triplicates on the maternal and fetal parts separately and subjected to one‐dimensional (1D) electrophoretic separation, followed by identification of peptide maps by nanospray liquid chromatography/tandem mass spectrometry (nanoLC/MS/MS). Proteins were identified by use of the MASCOT software with the SwissProt database. Results Proteomic analysis showed more than 4000 differentially expressed proteins in maternal and fetal parts of placenta. Each part expressed around 900 proteins, of which ca. 90 were common. The identified proteins were analyzed in accordance to molecular function and their participation in biological processes. Conclusions The obtained results provide new insight into the knowledge about biochemical characteristics of placenta (new proteins) and serve for further studies on the possible markers of physiological/pathological pregnancy or function of placenta. Moreover, our data can be a good starting point for further studies on the processes underlying the attachment of placenta.

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Composition of the bovine uterine proteome is associated with stage of cycle and concentration of systemic progesterone

Cited by 48 publications

References 117 publications

Interferon-tau and fertility in ruminants

Interferon-tau and fertility in ruminants

Proteome of the periovulatory oviduct and uterus of goats as related to nutritional balance

Identification of protein patterns in bovine placenta at early‐mid pregnancy – Pilot studies

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