Comprehensive evaluation of antioxidant activity for various substances with 5-axe cobweb chart

Myoglobin microplate assay to evaluate prevention of protein peroxidation

“…The myoglobin method is based on the measurement of the absorbance decay at 409 nm due to oxidation of heme group of protein induced by peroxyl radicals [17][18][19]. The UV-vis spectra of myoglobin solution at different reaction times (0, 30, 60, 90 and 120 min) in the presence of AAPH showed the decrease of absorbance peak (409 nm) of native myoglobin along reaction development (Supplementary data, Fig.…”

“…Myoglobin denaturation due to peroxidation is reflected in a decrease of absorbance at 409 nm (Soret band) owing to the exposure of the heme group, buried in a hydrophobic pocket within the protein's interior, to the polar aqueous solvent [18]. This probe was further applied toward different reactive species [19] as well as to determine antioxidant properties of flavonoids [20], leafy vegetables and beans [21] and commercially available miso [22]. However, for peroxyl radical scavenging capacity, samples were pre-incubated at 47 • C and the myoglobin protective ratios were calculated from the data after 30 min of reaction, not considering the kinetic information provided by the area under the curve.…”

Evaluation of Antioxidative Activity for Extracts from Defatted Rice Bran Using 5-Axe Cobweb Chart

“…The antioxidative activities of the extracts or acetone-soluble substances against hypochlorite and peroxynitrite ions, and the hydroxyl and peroxyl radicals were also assessed according to the methods proposed by Terashima et al (2010), which were based on the structural changes of myoglobin. Examination of the activity against hypochlorite and peroxynitrite ions, and hydroxyl and peroxyl radicals are expressed as CLO, ONOO, OH and AAPH tests, respectively.…”

“…Acetone-soluble substances were also prepared from extracts at room temperature. The antioxidative activity of the extracts and substances were then evaluated using the method of Terashima et al (2010).…”

“…However, the physiological significance of the activity is unknown and application of methods for evaluation of the antioxidants for food use is questionable because the DPPH radical does not exist within living organisms. Terashima et al (2010) proposed a novel method for evaluating the antioxidative activity of water-soluble antioxidants against the hydroxyl radical, the peroxyl radical, the hypochlorite ion, and the peroxynitrite ion based on the structural changes of myoglobin, as well as against DPPH radicals. The physiological functions of the reactive oxygen species have been elucidated (Valko et al, 2007).…”