Comprehensive profiling of flavonoids inScutellaria incanaL. using LC-Q-TOF-MS

Abstract

Summary. Scutellaria L. is a diverse genus of the Lamiaceae (Labiatae) family of over 300 herbaceous plants commonly known as skullcaps. Various species of Scutellaria are used as ethnobotanical herbs for the treatment of ailments like cancer, jaundice, cirrhosis, anxiety, and nervous disorders. Scutellaria incana L., commonly known as the Hoary skullcap, is a traditional medicinal plant used by native Americans as a sedative for nervousness or anxiety. S. incana metabolites were identified by comparing their highperformance liquid chromatography (HPLC) retention times and mass spectra with those of the corresponding authentic standards. Where standards were unavailable, the structures were characterized on the basis of their tandem mass spectrometry (MS/MS) spectra following collision-induced dissociation (CID) and the accurate masses of the corresponding deprotonated molecules [M-H] − (mass accuracy ± 5 ppm). A total of 40 flavonoids, including two phenolic glycosides, were identified from leaves, stems, and roots of S. incana. Differences in the flavonoid composition between leaves, stems, and roots in S. incana were observed although the flavonoid profile of S. incana is consistent with other Scutellaria species. Further work should focus on assessing the potential of S. incana as a source of these bioactive metabolites.

Chemical compositions and biological activities of Scutellaria pinnatifida A. Hamilt aerial parts

“…In the case of compounds (2) and (3) , UV spectra were identical with flavone monoglucoside ( 22 ), which was supported by 1 H-NMR and 13 C-NMR spectrums, showing characteristic signals appeared at δ H 6.70 (1H, s, H-3), 6.62 (1H, s, H-3), indicating the chromophore are flavones, luteolin, and apigenin, respectively. A literature survey indicated that luteolin-7-o-ß-D-glucoside (2) and apigenin-7-o-ß-D-glucoside (3) have previously been isolated from Scutellaria genus ( 23 24 ). The UV spectroscopic data of compound (4) revealed its phenolic nature.…”

A comparative assessment ofin vitrocytotoxic activity and phytochemical profiling ofAndrographis nallamalayanaJ.L.Ellis andAndrographis paniculata(Burm. f.) Nees using UPLC-QTOF-MS/MS approach

“…Based on fragmentation spectra, accurate mass and previous literature reports, compound 13 was tentatively identified as chrysin-7-glucuronide. 111 Compound 14 (peak 14) possessed molecular ion [M–H] − at m / z 461. Furthermore, the fragment ion at m / z 299 corresponds to the loss of glucose moiety, indicating the molecular ion of peonidin aglycone.…”

Full component analysis of Tianma-Gouteng-Yin

“…The peaks observed in the chromatograms of the TGY samples were primarily identified based on a comparison of their accurate molecular mass data with those of the reference standards and data from the literature [ 19 – 23 ]. In this way, we were able to identify 28 compounds.…”