Concentrating alkaline serine protease, subtilisin, using a temperature-sensitive hydrogel

Abstract

The alkaline serine protease, subtilisin, produced by Bacillus licheniformis was concentrated using hydrogel ultrafiltration. Separation efficiency at 15 ° and 200C was 84 % but decreased above 25°C.

Microspheres for Enzyme Immobilization

“…Various thermoresponsive gels were produced by copolymerization of NIPAM with different acrylate-based comonomers, and some of them have been proposed as support materials for the immobilization of enzymes (Han et al, 1995). Comonomers along with NIPAAm often include acrylic acid, MAA, 2-methyl-2-acrylamidopropane sulfonic acid, trimethyl-acrylamido propyl ammonium 3-methyl-1-vinylimidazolium iodide, sodium acrylate, sodium methacrylate, and 1-(3-sulphopropyl)-2-vinyl-pyridinium betaine (Lee & Shieh, 1999;Opan et al, 2002).…”

Delivery of lactase using chocolate-coated agarose carriers

“…Agarose appeared to be a better immobilization matrix for the peroxidase than sodium alginate (Nahakpam et al, 2008). In addition, although the use of drying can make immobilization more complicated since it might influence the entrapped encapsulate, especially if it is sensitive to heat treatment, dried agar gel beads have served as a research model for drying (Kubota, Kobatake, Suzuki, & Hosaka, 1977;Suzuki, Onishi, Esaka, Kubota, & Hosaka, 1989) and dried gel beads in general can also be used for the separation of enzymes (Han, Park, & Ruan, 1995). Drying has also been proven beneficial for the shelf life of beads that include drugs.…”

Immobilization of catalase in poly(isopropylacrylamide-co-hydroxyethylmethacrylate) thermally reversible hydrogels

“…Various thermoresponsive gels were produced by copolymerization of NIPAM with different acrylate based comonomers, and some of them have been proposed as support materials for the immobilization of enzymes and cells. 6,8±12 Copolymerization of NIPAM with HEMA made suitable materials for immobilization of enzymes because their LCST values were higher than that of pure pNIPAM. 11,12 In some cases, the biotechnological and biomedical applications of synthetic polymers may require functional groups for coupling of enzymes and other proteins.…”