Concentration and separation of hypoglycemic drugs using solid-phase extraction-capillary electrophoresis

Strausbauch, Michael; Xu, Shouming; Ferguson, Jayne E.; Núñez, Mireia; Machacek, Dwaine; Lawson, George M.; Wettstein, Peter J.; Landers, James P.

doi:10.1016/0021-9673(95)00743-6

Cited by 77 publications

(22 citation statements)

References 32 publications

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“…The choice of chromatographic material allows the SPE device to be tailored from being extremely selective by using an immunoaffinity ligand, through to being completely generic with a C18 or ion-exchange phase. Early reports on the integration of SPE and CE appeared in the 1990s by Guzman [214][215][216][217][218] and Landers and co-workers [219][220][221]. Both parties used small packed beds in a sleeve held between two pieces of capillaries, which could be placed directly in a commercial CE instrument.…”

Section: Spementioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

2007

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Poor sensitivity is considered to be one of the major limitations of electrophoretic separation methods, particularly when compared to traditional liquid chromatographic techniques. To address this issue, various in-line preconcentration techniques have been developed over the past 15 years, ranging in power and complexity, and there are now a number of well understood approaches routinely capable of providing a 10,000- to 100,000-fold increase in sensitivity, as well as several that can be pushed above a million. Furthermore, these have been achieved with particularly troublesome and often difficult samples, such as those having high salinity from a biological or environmental origin. This review will discuss the most common methods for improving the sensitivity of CE, CEC and microchip version of these, with particular attention to those approaches developed over the last five years.

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Section: Spementioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

2007

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“…Two types of applications have been reported using AC: characterization of metabolite and biopolymer mixtures [7][8][9][10][11] and determination of small molecules such as hypogly-cemic drugs [12], oxprenolol [13], verapamil [14], doxepin [15], terbutaline [16], ceftiofur [2], chlorophenols [17], and 3-nitrotyrosine [6]. Quantification was just attempted in case of small molecules and it was limited to only one or two compounds, using in all cases UV-Vis detection.…”

Section: Introductionmentioning

confidence: 99%

In‐line solid‐phase extraction preconcentration in capillary electrophoresis‐tandem mass spectrometry for the multiresidue detection of quinolones in meat by pressurized liquid extraction

et al. 2008

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We have developed and validated a CE-MS/MS method using an in-line SPE device (analyte concentrator, AC) to determine eight quinolones of veterinary use whose maximum residue levels in animal edible tissues are established by the EU Council Regulation 2377/90, i.e., danofloxacin, sarafloxacin, ciprofloxacin, marbofloxacin, enrofloxacin, difloxacin, oxolinic acid, and flumequine. Different parameters affecting the AC performance, such as its design (in this case frit-free), the kind of sorbent (Oasis MCX), sample pH, volume, and composition of the elution plug and injection time were studied. The method was validated using standard solutions obtaining LODs between 17 and 59 ng/L. Finally, a pressurized liquid extraction (PLE) method was developed to determine these antibiotics in chicken muscle samples. The whole analytical method was validated in terms of linearity (r2 >or= 0.992), recoveries (63-112%), repeatability and intermediate precision (RSD

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“…In-line SPME-CE was first demonstrated by Guzman et al [5] who used a small packed bed for the preconcentration of methamphetamine. Subsequently, the groups of Landers [6][7][8][9] and Tomlinson [10,11], continued the development of in-line SPME-CE, however, there were several complications with the use of packed beds. Firstly, it was difficult to make a suitable SPME column.…”

Section: Introductionmentioning

confidence: 99%

Coupling of solid‐phase microextraction continuous bed (monolithic) capillaries with capillary zone electrophoresis for direct analysis of drugs in biological fluids

et al. 2008

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Hyperlink robust biocompatible solid-phase microextraction (SPME) devices were prepared using continuous bed (monolithic) restricted-access media (RAM) as the SPME capillary insert. The RAM-based SPME approach was able to simultaneously separate proteins from a biological sample, while directly extracting the active components of caffeine, paracetamol and acetylsalicylic acid from the drug NeoCitramonum. The devices were interfaced with a CZE system and fully automated analysis for sample preconcentration, desorption, separation and quantification of analytes was evaluated. Comparative study of in-line coupled SPME-CZE using RAM and RP capillary inserts was carried out. Using an SPME (RAM) insert, the calculated caffeine, paracetamol and acetylsalicylic acid LODs in a bovine plasma sample were 0.3, 0.8 and 1.9 ng/mL, respectively.

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Concentration and separation of hypoglycemic drugs using solid-phase extraction-capillary electrophoresis

Cited by 77 publications

References 32 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

In‐line solid‐phase extraction preconcentration in capillary electrophoresis‐tandem mass spectrometry for the multiresidue detection of quinolones in meat by pressurized liquid extraction

Coupling of solid‐phase microextraction continuous bed (monolithic) capillaries with capillary zone electrophoresis for direct analysis of drugs in biological fluids

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