Concordance between qualitative and quantitative cultures in burned patients

Danilla, Stefan; Andrades, Patricio; Gómez, M.; Chamorro, Marcela; Léniz, Patricio; Piñeros, J.L.; Llanos, Sergio; Roco, H.; Correa, G.; Pasten, Juan A.; Eulufi, Alex; Roa, Ricardo; Calderón, Wilfredo

doi:10.1016/j.burns.2005.08.019

Cited by 23 publications

(6 citation statements)

References 15 publications

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“…These differences in sensitivity among RT-LAMP and RT-qPCR are in agreement with our own observations, as well as with those from other authors. Likewise, if the analysis of the results was limited to those samples with average Cq values below 35, as described in previous studies [9,34,37], only three samples would be classified as ND; thus, with this threshold set, the SP would remain at 99.0%, while the SE would increase up to 90.9%, the AC to 97.0%, the PPV and NPV to 96.8 and 97.1%, respectively, rendering a k of 0.92 that is interpreted as "very good concordance" among the reference and the alternative method [33]. The evaluation of the methodology focused on individuals with an active infection provided better results than those previously reported, where the analysis of a similar group of samples only provided sensitivity results of 72.7%, but it must be kept in mind that the type of clinical samples analyzed was not the same, saliva against nasopharyngeal swabs [35].…”

Section: Clinical Evaluationmentioning

confidence: 85%

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Optimization and Clinical Evaluation of a Multi-Target Loop-Mediated Isothermal Amplification Assay for the Detection of SARS-CoV-2 in Nasopharyngeal Samples

Roumani

Azinheiro

Sousa

et al. 2021

Viruses

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SARS-CoV-2 is the coronavirus responsible for COVID-19, which has spread worldwide, affecting more than 200 countries, infecting over 140 million people in one year. The gold standard to identify infected people is RT-qPCR, which is highly sensitive, but needs specialized equipment and trained personnel. The demand for these reagents has caused shortages in certain countries. Isothermal nucleic acid techniques, such as loop-mediated isothermal amplification (LAMP) have emerged as an alternative or as a complement to RT-qPCR. In this study, we developed and evaluated a multi-target RT-LAMP for the detection of SARS-CoV-2. The method was evaluated against an RT-qPCR in 152 clinical nasopharyngeal swab samples. The results obtained indicated that both assays presented a “good concordance” (Cohen’s k of 0.69), the RT-LAMP was highly specific (99%) but had lower sensitivity compared to the gold standard (63.3%). The calculated low sensitivity was associated with samples with very low viral load (RT-qPCR Cq values higher than 35) which may be associated with non-infectious individuals. If an internal Cq threshold below 35 was set, the sensitivity and Cohen’s k increased to 90.9% and 0.92, respectively. The interpretation of the Cohen’s k for this was “very good concordance”. The RT-LAMP is an attractive approach for frequent individual testing in decentralized setups.

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Section: Clinical Evaluationmentioning

confidence: 85%

“…k: index kappa of concordance. Interpretation of k: 0.4-0.6 "moderate concordance"; 0.61-0.8 "good concordance"; 0.81-1.00 "very good concordance" according to[33].…”

mentioning

confidence: 99%

Optimization and Clinical Evaluation of a Multi-Target Loop-Mediated Isothermal Amplification Assay for the Detection of SARS-CoV-2 in Nasopharyngeal Samples

Roumani

Azinheiro

Sousa

et al. 2021

Viruses

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“…The superficial swab was chosen to collect samples, in order to identify the commonly isolated microorganisms from the burn wound. This method was simple, inexpensive and less painful [19]. A previous study had shown no significant differences in bacterial counts between full thickness and partial thickness burns, whether by surface swab or by biopsy [20].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial properties of tualang honey and its effect in burn wound management: a comparative study

Nasir

Halim

Singh

et al. 2010

BMC Complement Altern Med

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BackgroundThe use of honey as a natural product of Apis spp. for burn treatment has been widely applied for centuries. Tualang honey has been reported to have antibacterial properties against various microorganisms, including those from burn-related diagnoses, and is cheaper and easier to be absorbed by Aquacel dressing. The aim of this study is to evaluate the potential antibacterial properties of tualang honey dressing and to determine its effectiveness as a partial thickness burn wound dressing.MethodsIn order to quantitate the bioburden of the swabs, pour plates were performed to obtain the colony count (CFU/ml). Swabs obtained from burn wounds were streaked on blood agar and MacConkey agar for bacterial isolation and identification. Later, antibacterial activity of Aquacel-tualang honey, Aquacel-Manuka honey, Aquacel-Ag and Aquacel- plain dressings against bacteria isolated from patients were tested (in-vitro) to see the effectiveness of those dressings by zone of inhibition assays.ResultsSeven organisms were isolated. Four types of Gram-negative bacteria, namely Enterobacter cloacae, Klebsiella pneumoniae, Pseudomonas spp. and Acinetobacter spp., and three Gram-positive bacteria, namely Staphylococcus aureus, coagulase-negative Staphylococcus aureus (CONS) and Streptococcus spp., were isolated. Total bacterial count decreased on day 6 and onwards. In the in-vitro antibacterial study, Aquacel-Ag and Aquacel-Manuka honey dressings gave better zone of inhibition for Gram positive bacteria compared to Aquacel-Tualang honey dressing. However, comparable results were obtained against Gram negative bacteria tested with Aquacel-Manuka honey and Aquacel-Tualang honey dressing.ConclusionsTualang honey has a bactericidal as well as bacteriostatic effect. It is useful as a dressing, as it is easier to apply and is less sticky compared to Manuka honey. However, for Gram positive bacteria, tualang honey is not as effective as usual care products such as silver-based dressing or medical grade honey dressing.

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“…Studies, including work performed in our laboratory and in a clinical setting, have consistently shown that careful quantitative cultures are reproducible, more reliable, and more accurate than the traditional qualitative approaches (Danilla et al, 2005). The superiority of quantitative cultures is related to specimen acquisition and standardization.…”

Section: Clinical Approaches To Samplingmentioning

confidence: 97%

Molecular Microbiology: New Dimensions for Cutaneous Biology and Wound Healing

Martin

Zenilman

Lazarus

2010

Journal of Investigative Dermatology

140

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The role of bacteria in the pathogenesis of chronic, nonhealing wounds is unclear. All wounds are colonized with bacteria, but differentiating colonizers from invading organisms is difficult, if not impossible, at the present time. Furthermore, robust new molecular genomic techniques have shown that only 1% of bacteria can be grown in culture; anaerobes are especially difficult to identify using standard culture methods. Recent studies utilizing microbial genomic methods have demonstrated that chronic wounds are host to a wide range of microorganisms. New techniques also show that microorganisms are capable of forming highly organized biofilms within the wound that differ dramatically in gene expression and phenotype from bacteria that are typically seen in planktonic conditions. The aim of this review is to present a concise description of infectious agents as defined by new molecular techniques and to summarize what is known about the microbiology of chronic wounds in order to relate them to the pathophysiology and therapy of chronic wounds.

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Concordance between qualitative and quantitative cultures in burned patients

Cited by 23 publications

References 15 publications

Optimization and Clinical Evaluation of a Multi-Target Loop-Mediated Isothermal Amplification Assay for the Detection of SARS-CoV-2 in Nasopharyngeal Samples

Optimization and Clinical Evaluation of a Multi-Target Loop-Mediated Isothermal Amplification Assay for the Detection of SARS-CoV-2 in Nasopharyngeal Samples

Antibacterial properties of tualang honey and its effect in burn wound management: a comparative study

Molecular Microbiology: New Dimensions for Cutaneous Biology and Wound Healing

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