Concurrent enhancement of imaging depth and contrast for optical coherence tomography by hyperosmotic agents

Wang, Ke; Xu, Xiaodong; Tuchin, Valery V.; Elder, J. Bradley

doi:10.1364/josab.18.000948

Cited by 172 publications

(144 citation statements)

References 18 publications

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“…[2][3][4] In particular, cutaneous blood perfusion is highly correlated to a number of peripheral vascular diseases, but the optical imaging of cutaneous blood vessels is quite challenging due to the high scattering property of skin. By matching the refractive index between different tissue components, tissue optical clearing has been found useful for reducing light scattering and improving imaging performance in a wide range of optical techniques, such as laser speckle contrast imaging (LSCI), 5,6 optical coherence tomography (OCT), 7,8 photoacoustic microscopy, 9,10 confocal microscopy, 11,12 two-photon microscopy, 13,14 and flow cytometry. 15 Extensive efforts have been devoted to the screening of highefficiency and biocompatible optical clearing agents (OCAs) or chemical penetration enhancers for improving the diffusion of OCAs within tissues.…”

Section: Introductionmentioning

confidence: 99%

Optical coherence tomography angiography offers comprehensive evaluation of skin optical clearingin vivoby quantifying optical properties and blood flow imaging simultaneously

Shi

Zhang

et al. 2016

J. Biomed. Opt

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Abstract. Tissue optical clearing (TOC) is helpful for reducing scattering and enhancing the penetration depth of light, and shows promising potential in optimizing optical imaging performances. A mixture of fructose with PEG-400 and thiazone (FPT) is used as an optical clearing agent in mouse dorsal skin and evaluated with OCT angiography (Angio-OCT) by quantifying optical properties and blood flow imaging simultaneously. It is observed that FPT leads to an improved imaging performance for the deeper tissues. The imaging performance improvement is most likely caused by the FPT-induced dehydration of skin, and the reduction of scattering coefficient (more than ∼40.5%) and refractive-index mismatching (more than ∼25.3%) in the superficial (epidermal, dermal, and hypodermal) layers. A high correlation (up to ∼90%) between the relative changes in refractive-index mismatching and Angio-OCT signal strength is measured. The optical clearing rate is ∼5.83 × 10 −5 cm∕s. In addition, Angio-OCT demonstrates enhanced performance in imaging cutaneous hemodynamics with satisfactory spatiotemporal resolution and contrast when combined with TOC, which exhibits a powerful practical application in studying microcirculation. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Introductionmentioning

confidence: 99%

Optical coherence tomography angiography offers comprehensive evaluation of skin optical clearingin vivoby quantifying optical properties and blood flow imaging simultaneously

Shi

Zhang

et al. 2016

J. Biomed. Opt

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“…1 The effects of optical clearing of highly scattering biological tissue by using the optical clearing agents ͑OCAs͒-e.g., glycerol ͑G͒, 2-4 glucose, [5][6][7] and dimethyl sulfoxide 8,9 -have been investigated using many techniques, examples of which include near-infrared ͑NIR͒ spectrophotometers 10,11 and optical coherence tomography ͑OCT͒ imaging. [12][13][14][15][16][17] OCT is a recently developed imaging technique that has the potential for early diagnosis of diseases in human tissues. 18,19 It has very high axial resolution, but the high scattering nature of nontransparent human tissue limits the imaging depth of OCT to 1 to 2 millimeters.…”

Section: Introductionmentioning

confidence: 99%

In vitro study of ultrasound and different-concentration glycerol–induced changes in human skin optical attenuation assessed with optical coherence tomography

et al. 2010

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Abstract. Previous studies have demonstrated the ultrasound-induced skin optical clearing enhancement with topical application of 60% glycerol ͑G͒ on in vitro porcine skin and in vivo human skin. Our purpose was to find the relation between the effect of optical skin clearing and different concentrations of glycerol and to find more effective ultrasound-glycerol combinations on optical skin clearing. The enhancement effect of ultrasound ͓Sonophoresis ͑SP͒ delivery͔ in combination with 40% G, 60% G, and 80% G on in vitro human skin optical clearing was investigated. Light imaging depths of skin were measured using optical coherence tomography. Different concentrations of glycerol and ultrasound with a frequency of 1 MHz and an intensity of 0.5 W / cm 2 was simultaneously applied for 15 min. The results show that with the increase of concentration of glycerol, the optical clearing of skin is much improved. Optical clearing capability of glycerol was more enhanced with simultaneous application of ultrasound compared with glycerol alone. The attenuation coefficients of skin tissues after application of 40% G/SP, 60% G/SP, and 80% G/SP decreased approximately 11.8%, 18.5%, and 20.0% at 15 min compared with 40% G, 60% G, and 80% G alone, respectively. The greatest decrease in attenuation coefficients at 60 min was approximately 52.3% and 63.4% for 80% G ͑without ultrasound͒ and 80% G/SP ͑with ultrasound͒, respectively, which are 2.1-fold and 2.6-fold to that in the 40% G.

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“…Hyperosmotic agents result in refractive index matching between cells and ground substance in the dermis, which is believed to cause a reduction in optical scattering. Studies done by Vargas et al [3] and Wang et al [4], demonstrated that the injection of hyperosmotic agents into rat dermis could significantly reduce light scattering and thereby enhance the imaging depth of non-invasive techniques such as optical coherence tomography (OCT). If delivered by injection into the dermis, these hyperosmotic agents tend to dehydrate the skin and reduce the index mismatch between inter/intracellular components [5].…”

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confidence: 99%

Optical clearing of in vivo human skin: Implications for light‐based diagnostic imaging and therapeutics

et al. 2004

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Concurrent enhancement of imaging depth and contrast for optical coherence tomography by hyperosmotic agents

Cited by 172 publications

References 18 publications

Optical coherence tomography angiography offers comprehensive evaluation of skin optical clearingin vivoby quantifying optical properties and blood flow imaging simultaneously

Optical coherence tomography angiography offers comprehensive evaluation of skin optical clearingin vivoby quantifying optical properties and blood flow imaging simultaneously

In vitro study of ultrasound and different-concentration glycerol–induced changes in human skin optical attenuation assessed with optical coherence tomography

Optical clearing of in vivo human skin: Implications for light‐based diagnostic imaging and therapeutics

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