Concurrent photoacoustic and ultrasound microscopy with a coaxial dual-element ultrasonic transducer

Tang, Yuqi; Liu, Wei; Li, Yang; Zhou, Qifa; Yao, Junjie

doi:10.1186/s42492-018-0003-4

Cited by 7 publications

(2 citation statements)

References 45 publications

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“…For peripheral vascular visualization, B. Rao et al reported an optical resolution-PAM (OR-PAM) system to visualize mouse ear microvasculatures [ 32 ]. A dual-modality imaging system combining PAM with USI was reported by Y. Tang [ 33 ] to achieve anatomical and functional information of a mouse’s hind paw. Moreover, aiming towards human implementation, a PAM-based system for human peripheral arteries was developed [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Pilot Study: Quantitative Photoacoustic Evaluation of Peripheral Vascular Dynamics Induced by Carfilzomib In Vivo

Thao

Chu

et al. 2021

Sensors

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Carfilzomib is mainly used to treat multiple myeloma. Several side effects have been reported in patients treated with carfilzomib, especially those associated with cardiovascular events, such as hypertension, congestive heart failure, and coronary artery disease. However, the side effects, especially the manifestation of cardiovascular events through capillaries, have not been fully investigated. Here, we performed a pilot experiment to monitor peripheral vascular dynamics in a mouse ear under the effects of carfilzomib using a quantitative photoacoustic vascular evaluation method. Before and after injecting the carfilzomib, bortezomib, and PBS solutions, we acquired high-resolution three-dimensional PAM data of the peripheral vasculature of the mouse ear during each experiment for 10 h. Then, the PAM maximum amplitude projection (MAP) images and five quantitative vascular parameters, i.e., photoacoustic (PA) signal, diameter, density, length fraction, and fractal dimension, were estimated. Quantitative results showed that carfilzomib induces a strong effect on the peripheral vascular system through a significant increase in all vascular parameters up to 50%, especially during the first 30 min after injection. Meanwhile, bortezomib and PBS do not have much impact on the peripheral vascular system. This pilot study verified PAM as a comprehensive method to investigate peripheral vasculature, along with the effects of carfilzomib. Therefore, we expect that PAM may be useful to predict cardiovascular events caused by carfilzomib.

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Section: Introductionmentioning

confidence: 99%

Pilot Study: Quantitative Photoacoustic Evaluation of Peripheral Vascular Dynamics Induced by Carfilzomib In Vivo

Thao

Chu

et al. 2021

Sensors

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“…As an emerging biomedical imaging technique, optical-resolution photoacoustic microscopy (OR-PAM) has a sub-cellular resolution, rich optical absorption contrasts, and label-free imaging ability [ [1] , [2] , [3] , [4] , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] ]. Fast speed is of great importance for improving throughput and the study of physiological or pathological changes in vivo, such as brain activities, drug responses, and tumor metastasis processes, et al [ [13] , [14] , [15] , [16] , [17] ].…”

Section: Introductionmentioning

confidence: 99%

Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

et al. 2021

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We report fiber-based dual-foci fast-scanning OR-PAM that can double the scanning rate without compromising the imaging resolution, the field of view, and the detection sensitivity. To achieve fast scanning speed, the OR-PAM system uses a single-axis water-immersible resonant scanning mirror that can confocally scan the optical and acoustic beams at 1018 Hz with a 3-mm range. Pulse energies of 45∼100-nJ are sufficient for acquiring vascular and oxygen-saturation images. The dual-foci method can double the B-scan rate to 2036 Hz. Using two lasers and stimulated Raman scattering, we achieve dual-wavelength excitation on both foci, and the total A-line rate is 3.2-MHz. In in vivo experiments, we inject epinephrine and monitor the hemodynamic and oxygen saturation response in the peripheral vessels at 1.7 Hz over 2.5 × 6.7 mm 2 . Dual-foci OR-PAM offers a new imaging tool for the study of fast physiological and pathological changes.

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Optically Transparent Focused Transducers for Combined Photoacoustic and Ultrasound Microscopy

2020

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Purpose Photoacoustic (PA) microscopy has emerged as a useful tool in biomedical imaging applications such as visualization of microvasculature and hemoglobin oxygen saturation, single-cell, and label-free imaging of organs including cancer. Since the ultrasound transducers used for PA signal detection are not optically transparent, the integration of optical and acoustic modules for coaxial alignment of laser and acoustic beam fields in PA microscopy is complex and costly. Methods Here, we report a recently developed optically transparent focused transducer for combined PA and ultrasound (US) microscopy. All the acoustic layers including the acoustic lens are optically transparent, enabling simple integration of optical and acoustic modules for both imaging modalities. Results The mean light transmittance of the transducer’s backing layer and acoustic lens and of the transducer itself were measured at 92%, 83%, and 66%, respectively. Results from in vitro and in vivo experiments demonstrated the transducer to be suitable for both US and PA imaging. Conclusions The results of this study represent a step toward efficient construction of probes for combined PA and US microscopy.

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Concurrent photoacoustic and ultrasound microscopy with a coaxial dual-element ultrasonic transducer

Cited by 7 publications

References 45 publications

Pilot Study: Quantitative Photoacoustic Evaluation of Peripheral Vascular Dynamics Induced by Carfilzomib In Vivo

Pilot Study: Quantitative Photoacoustic Evaluation of Peripheral Vascular Dynamics Induced by Carfilzomib In Vivo

Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

Optically Transparent Focused Transducers for Combined Photoacoustic and Ultrasound Microscopy

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