Conjugated Polyelectrolyte‐Antibody Hybrid Materials for Highly Fluorescent Live Cell‐Imaging

Lee, Kangwon; Lee, Jiseok; Jeong, Eun Jeong; Kronk, Adam; Elenitoba-Johnson, Kojo S.J.; Lim, Megan S.; J, Kim

doi:10.1002/adma.201103895

Cited by 52 publications

(44 citation statements)

References 52 publications

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“…Compared with the corresponding monomers, the absorption spectra of P1∼P4 ( Figure S1) not only have characteristic absorptions of monomers but also exhibit new combination absorptions with the increase of aromatic heterocyclic units. [12][13][14][15][16][17][18][19][20][21][22] For the targeted imaging and detection of tumor cells, the specifi city for the reported CPN systems remains unsatisfi ed due to the single antibody recognition mode to tumor cells. The emission spectra show maxima for P1, P2, P3 and P4 are 422, 500, 540 and 670 nm, respectively.…”

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Preparation and Biofunctionalization of Multicolor Conjugated Polymer Nanoparticles for Imaging and Detection of Tumor Cells

et al. 2014

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enced by changing the choice, amount and ratio within each CPN. [ 7,26 ] Additionally, as single multicolor CPNs can be tuned to match the different excitation sources of commercial optical instruments, such as fl uorescence spectrometers, fl uorescence microscopes, and in fl ow cytometry.In this work, we describe the design and synthesis of four conjugated polymers with blue, green, yellow and red emissions, and used them to prepare carboxyl functionalized CPNs by a co-precipitation method based on hydrophobic interactions between the conjugated polymers and poly(styreneco -maleic anhydride) (PSMA). [ 6,27 ] The carboxyl functionalized CPNs can also be prepared by co-precipitation of four conjugated polymers with PSMA, which show multicolor emissions by under single excitation wavelength. CPNs were modifi ed with primary antibody to obtain CPNs-antibody conjugates. In comparison to single antibody recognition, higher specifi city for tumor cells detection was achieved by binding two CPNs labeled with different antibodies to a single tumor cell. Finally, we show that the multicolor CPNs can match different excitation sources of fl uorescence microscopy and fl ow cytometry to achieve straightforward cell imaging and detection.Four π-conjugated polymers (P1 ∼ P4) were obtained by Suzuki cross-coupling polymerization of monomers 1 -4 with monomer 5 [ 17,28 ] in yields of 15∼48% ( Scheme 1 a). Absorption and fl uorescence characteristics were determined in CHCl 3 and show that each polymer spans a different region of the visible spectrum (Table S1). Compared with the corresponding monomers, the absorption spectra of P1∼P4 ( Figure S1) not only have characteristic absorptions of monomers but also exhibit new combination absorptions with the increase of aromatic heterocyclic units. In these polymers, the absorptions in shorter wavelength region originate from the fl uorene units, and the longer absorptions lie in the signal aromatic heterocyclic unit or cooperative actions of different monomers. The emission spectra show maxima for P1, P2, P3 and P4 are 422, 500, 540 and 670 nm, respectively. Emission quantum yields (QY) vary depending on the electron acceptor unit in the polymer backbones (3 ∼ 78%). Upon excitation at 360 nm, the shorter wavelength-emissive polymers were anticipated to act as the donor for longer wavelength-emissive ones (acceptors), through interchain multi-step FRET. Thus, by varying the mixing ratio of the polymers, multicolor emission can be regulated through FRET among the four polymers under one excitation wavelength. In order to achieve bioconjugation, CPNs are required to provide surface functional groups (such as -COOH, -NH 2 , -N 3 , -C≡CH, etc.) for subsequent modifi cation. Herein, such CPNs with carboxyl groups on the surface were prepared by a modifi ed co-precipitation method [ 6,27 ] based Conjugated polymer nanoparticles (CPNs) combine the properties of conjugated polymers (CPs) and nanoparticles and defi ne a new class of promising fl uorescence materials that are being integrated in...

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Preparation and Biofunctionalization of Multicolor Conjugated Polymer Nanoparticles for Imaging and Detection of Tumor Cells

et al. 2014

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“…[1] These water-soluble CPEs have been used as light-harvesting molecules because of their delocalized electronic structure and bio-sensor due to the interaction between ionic side groups and biological compounds. [2,3] In recent years, CPEs have been reported on bio-applications such as DNA sensor, [4] DNA microarray, [5] protein sensor, [6] bio-imaging, [7] as well as optoelectronic applications such as organic lightemitting diodes, [8] organic semiconductors and actuators. [9,10] The widely used CPEs are mainly based on several conjugated polymer backbones, including polythiophene (PT), [11] fluorene-benzene copolymers(PFP), [12] polyphenylene (PPP), and polyphenylene vinylene (PPV).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a novel water-soluble conjugated polyelectrolyte based on polycyclopentadithiophene backbone and its application for heparin detection

Zhu

Guo

et al. 2014

Designed Monomers and Polymers

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Synthesis of a novel water-soluble conjugated polyelectrolyte based on polycyclopentadithiophene backbone and its application for heparin detection, Designed Monomers and Polymers, 17:7, 624-628,The novel water-soluble conjugated polyelectrolyte containing a new kind of backbone poly [(4,4-bis(3ʹ-N,N,N-trimethylammonium) propyl) cyclopentadithiophene dichloride] (PBTPC-Cl) is synthesized and characterized. The PBTPC-Cl is readily soluble in polar solvents and is applied as a sensitive and selective fluorescent probe for heparin detection in aqueous solution. Upon adding heparin into the aqueous solution of polymer, PBTPC-Cl could bind heparin via electrostatic interactions and results in the quenching of emission peaks. Quantitative analysis of heparin shows high selectivity and good linear relationship with a detection limit to 0.05 μM in HEPES aqueous buffer.

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“…A range of cationic and anionic CPEs have been synthesized for specific applications including bioimaging, bioelectronics, fluorescent probes and signaling mediation. 12–17 Structural diversity allows the optical and electronic properties of CPEs to be adjustable and consequently takes advantage of their light harvesting properties. 18,19 …”

Section: Introductionmentioning

confidence: 99%

Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays

et al. 2015

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Conjugated Polyelectrolyte‐Antibody Hybrid Materials for Highly Fluorescent Live Cell‐Imaging

Cited by 52 publications

References 52 publications

Preparation and Biofunctionalization of Multicolor Conjugated Polymer Nanoparticles for Imaging and Detection of Tumor Cells

Preparation and Biofunctionalization of Multicolor Conjugated Polymer Nanoparticles for Imaging and Detection of Tumor Cells

Synthesis of a novel water-soluble conjugated polyelectrolyte based on polycyclopentadithiophene backbone and its application for heparin detection

Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays

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