Consecutive monitoring for conidiogenesis by Oidium neolycopersici on tomato leaves with a high-fidelity digital microscope

Oichi, Wataru; Matsuda, Yoshinori; Sameshima, Takeshi; Nonomura, Teruo; Kakutani, Koji; Nishimura, Hiroaki; Kusakari, Shin-ichi; Toyoda, Hideyoshi

doi:10.1007/s10327-004-0136-5

Cited by 21 publications

(13 citation statements)

References 16 publications

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“…A high-fidelity digital microscope has been used in our laboratory to consecutively observe fungal morphogenesis, such as conidiophore formation (Oichi et al 2004Moriura et al 2006;Nonomura et al 2009a) and conidial germination, by the tomato powdery mildew on the leaf surface (Matsuda et al 2005a;Nonomura et al 2009a, b) and dynamic water absorption by leaf surface trichomes of tomato (Nonomura et al 2009b). With this microscopic technique, one could also observe three-dimensional directions of continuous germ tube development by the conidia on the rugged leaf surface of tomato (Nonomura et al 2009a).…”

Section: Introductionmentioning

confidence: 97%

“…Recently, Cook & Braun (2009) proposed appressorial germ tube patterns to provide a key for the identification of Oidium genera based on germination types. In our studies of the tomato powdery mildew Oidium neolycopersici, the shapes of the appressoria (Matsuda et al 2001;Kashimoto et al 2003) and the types of conidial production (Oichi et al 2004Nonomura et al 2009a) were the first clues to understanding the development of infection structures by the pathogen. Appressoria occur with nipple-shaped to lobed outgrowths of the germ tubes, forming structures that attach the mycelium to the host surface and initiate the haustoria (Braun et al 2002).…”

Section: Introductionmentioning

confidence: 97%

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Polymorphic change of appressoria by the tomato powdery mildew Oidium neolycopersici on host tomato leaves reflects multiple unsuccessful penetration attempts

et al. 2010

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Polymorphic change of appressoria by the tomato powdery mildew Oidium neolycopersici on host tomato leaves reflects multiple unsuccessful penetration attempts

et al. 2010

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“…Previously, we clarified the germination behaviour of non‐fixed living powdery mildew conidia using a high‐fidelity digital microscope (Oichi et al . ; Matsuda et al . ; Nonomura et al .…”

Section: Introductionmentioning

confidence: 99%

Targeted destruction of fungal structures of Erysiphe trifoliorum on flat leaf surfaces of Marchantia polymorpha

et al. 2013

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In this study, we observed the germination behaviour of airborne conidia from powdery mildews that settle on thalloid surfaces. We inoculated thalli (flat, sheet-like leaf tissues) and gemmae (small, flat, sheet-like leaf tissues that propagate asexually via bud-like structures) of the common liverwort (Marchantia polymorpha) with conidia from tomato powdery mildew (Oidium neolycopersici; KTP-02) and red clover powdery mildew (Erysiphe trifoliorum; KRCP-4N) and examined their germination and subsequent appressorium formation under a high-fidelity digital microscope. Conidial bodies and germ tubes of the inoculated KRCP-4N conidia were destroyed on both the thalli and gemmae. The destruction of these fungal structures was observed only for KRCP-4N conidia inoculated onto M. polymorpha on both leaf surfaces. No differences in destruction of the KRCP-4N fungal structures between thalli and gemmae were observed. At 4 h post-inoculation, destruction of the germ tube tip was observed when it reached the gemmae leaf surface. At 6 h post-inoculation, the conidial bodies and germ tubes were destroyed. In contrast, KTP-02 conidia were not destroyed and formed normal, well-lobed appressoria on the surface of M. polymorpha gemmae.

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“…The corona discharge generator described in this study is portable and easy to operate, and the exposure treatment could be integrated as a part of routine crop care; the corona discharge exposure can be applied as soon as powdery mildew colonies are visible. In these colonies, conidia on conidiophores are immature, so killing them at this stage effectively suppresses the subsequent production and release of mature conidia (Oichi et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Physical control of powdery mildew (Oidium neolycopersici) on tomato leaves by exposure to corona discharge

Nonomura

Matsuda

Kakutani

et al. 2008

Canadian Journal of Plant Pathology

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We devised a cylindrical electrostatic discharge generator to physically eradicate tomato powdery mildew colonizing tomato leaves. The generator consists of a copper needle with a pointed tip, an insulating acrylic cylinder, and an electrostatic voltage generator. The needle is insulated with a vinyl sleeve, except for the pointed tip, and is coaxially fixed in the cylinder and connected to the voltage generator. The needle is negatively charged, and the treated plant is earthed. In initial tests, a corona, characterized by a blue glow, formed at the needle tip as the probe was brought closer to the leaf surface. The distance at which this occurred increased from 16 to 50 mm as the voltage was increased from 5 to 30 kV. If the probe was brought too close to the leaf surface, an arc discharge occurred that caused injury to the leaf. Powdery mildew colonies were destroyed by 2-second exposures at probe distances intermediate to where corona discharge was initiated and where arcing occurred. A probe distance of 25 mm and 30 kV for a 2-second burst was selected to further test the efficacy of the probe for controlling powdery mildew in a greenhouse environment. Tomato plants were grown hydroponically in two open-window greenhouses under a first-truss cropping system. Colonies appeared on tomato leaves 10 to 14 days after transplanting. During the following 2 weeks, these colonies produced abundant progeny conidia that secondarily infected neighboring plants. Corona discharge treatment in one greenhouse, at the stage when colonies first became visible, completely suppressed the spread of the disease compared with a non-treated greenhouse in which disease spread rapidly. The present discharge generator is portable and easy to operate on-site as a part of routine care of hydroponically cultured tomatoes in greenhouses and provides a non-chemical method to control powdery mildew disease.Key words: disease control, tomato powdery mildew, electrostatic field, single-truss cropping. Nonomura et al.: powdery mildew on tomato / disease control / electrostatic discharge 524 Résumé : Nous avons conçu un générateur cylindrique de décharges électrostatiques pour éradiquer physiquement l'oïdium colonisant les feuilles de la tomate. Le générateur est composé d'une aiguille de cuivre pointue à une extrémité, d'un cylindre isolant d'acrylique et d'un générateur de voltage électrostatique. L'aiguille, sauf sa pointe, est isolée à l'aide d'un manchon de vinyle et est fixée coaxialement dans le cylindre et branchée au générateur. L'aiguille est chargée négativement et la plante est reliée à la terre. Lors des tests initiaux, un effluve, caractérisé par une lueur bleue, s'est formé à la pointe de l'aiguille lorsqu'on approcha la sonde de la surface d'une feuille. La distance à laquelle ceci s'est produit a augmenté de 16 à 50 mm quand le voltage a été augmenté de 5 à 30 kV. Si l'on approchait la sonde trop près de la surface de la feuille, il se produisait une décharge en arc qui blessait la feuille. Deux secondes d'exposition suffisaien...

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Consecutive monitoring for conidiogenesis by Oidium neolycopersici on tomato leaves with a high-fidelity digital microscope

Cited by 21 publications

References 16 publications

Polymorphic change of appressoria by the tomato powdery mildew Oidium neolycopersici on host tomato leaves reflects multiple unsuccessful penetration attempts

Polymorphic change of appressoria by the tomato powdery mildew Oidium neolycopersici on host tomato leaves reflects multiple unsuccessful penetration attempts

Targeted destruction of fungal structures of Erysiphe trifoliorum on flat leaf surfaces of Marchantia polymorpha

Physical control of powdery mildew (Oidium neolycopersici) on tomato leaves by exposure to corona discharge

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