Conservação e teste de tetrazólio em sementes de jabuticabeira

Hossel, Cristiano; Oliveira, Jéssica Scarlet Marth Alves de; Fabiane, Keli Cristina; Júnior, Américo Wagner; Citadin, Idémir

doi:10.1590/s0100-29452013000100029

Cited by 8 publications

(11 citation statements)

References 6 publications

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“…Study undertaken by Masetto et al (2009), for "pitanga-domato" (Eugenia pleurantha), namely, a species of the same genus, demonstrated that the optimal combination of the tetrazolium test (0.100% for 4 hours) also differed from this study. The differences were even more pronounced for species of different genera but of the same family (Myrtaceae) from the ones of the present study, as seen by Hössel et al (2013) for jabuticabeira (1.000% for 24 hours) and by Sarmento et al (2013) for pineapple guava (0.500% for 4 hours). Other studies also revealed differences between the species, as seen by Lamarca et al (2009) for seeds of brazilwood (0.050% for 2 hours), by Ribeiro et al (2010) for seeds of macaúba (0.500% for 4 hours) and by Carvalho et al (2014) for seeds of sorghum (0.100% for 3 hours).…”

Section: Resultssupporting

confidence: 45%

“…Thus, within this context, is highlighted the tetrazolium test, as already shown for seeds of brazilwood (Caesalpinia echinata) (Lamarca et al, 2009), sunflower (Helianthus annuus) , jabuticaba (Plinia cauliflora) (Hössel et al, 2013) and pineapple guava (Acca sellowiana) (Sarmento et al, 2013). The tetrazolium test is a rapid test for estimating the vigor and viability of seeds.…”

Section: Introductionmentioning

confidence: 79%

“…Several studies show the efficiency of the tetrazolium test to diagnose the state of physiological quality of seeds, as observed by Lamarca et al (2009) for seeds of brazilwood, subjected to accelerated aging, by Rego et al (2013) for seeds of guaçatonga (Casearia sylvestris Sw.) and "murta" (Myrtus L.), subjected to controlled drying, by Azerêdo et al (2011) for seeds of "angico-de-bezerro", by Hössel et al (2013) for seeds of jabuticabeira and by Sarmento et al (2013) for seeds of pineapple guava, subjected to different storage conditions and times.…”

Section: Resultsmentioning

confidence: 99%

“…Regarding forest species, studies described in the literature demonstrate the advances in methodological adequacy test for seeds, but it is not possible to standardize the methodology for implementing the same, since each species needs its own processes, as seen by changes in the concentration optimal tetrazolium solution, for example, 0.050 % for Brazilian firetree or Brazilian fern tree (Schizolobium parahyba) (Ferreira et al, 2007) and brazilwood (Lamarca et al, 2009), 0.075% for angico-de-bezerro (Piptadenia moniliformis Benth.) (Azerêdo et al, 2011), 0.100% for pitanga-do-mato (Myrcia crocea) (Masetto et al, 2009) and jabuticabeira (Hössel et al, 2013) and 0.500% for macaúba (Acrocomia aculeata and A. vinifera) (Ribeiro et al, 2010) and pineapple guava (Sarmento et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Methodology of the tetrazolium test for assessing the viability of seeds of Eugenia brasiliensis Lam., Eugenia uniflora L. and Eugenia pyriformis Cambess

Lamarca

Barbedo

2014

J. Seed Sci.

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-Eugenia brasiliensis Lam. ("grumixameira"), Eugenia uniflora L. ("pitangueira") and Eugenia pyriformis Cambess. ("uvaieira") are forest and fruit species of pharmacological and gastronomic potential and have desiccation-sensitive seeds. The development of quick viability tests for the assessment of physiological quality of these seeds is needed. The tetrazolium test is an important method providing fast assessment of the seed physiological quality. Thus, this work aimed to develop a method for the tetrazolium test for determining viability of "grumixameira," "pitangueira" and "uvaieira" seeds. Initially the seeds of these species were soaked in water for 3 h at 25 ºC. Three concentrations of tetrazolium solutions were analyzed (0.100 %, 0.125 % and 0.250 %) for seed staining using three incubation periods (2, 3 and 6 h) at 35 ºC in the dark. After staining, seed viability was determined. Then, the seeds were subjected to different levels of controlled drying and were analyzed for their physiological quality by germination and electrical conductivity tests. The tetrazolium test is a suitable tool for determining viability after staining the seeds at 35 ºC using a 0.250 % concentration solution for 3 hours for "grumixameira" seeds, 0.125 % for 3 hours for "pitangueira" seeds and 0.100 % for 2 hours for "uvaieira" seeds.Index terms: forest seeds, recalcitrant seeds, drying, viability test.Metodologia do teste de tetrazólio para a avaliação da viabilidade de sementes de Eugenia brasiliensis Lam., Eugenia uniflora L. e Eugenia pyriformis Cambess RESUMO -Grumixameira (Eugenia brasiliensis Lam.), pitangueira (Eugenia uniflora L.) e uvaieira (Eugenia pyriformis Cambess.) são espécies florestais e frutíferas de potencial farmacológico e gastronômico e apresentam sementes sensíveis à dessecação. O teste de tetrazólio é um importante componente para a rápida avaliação da viabilidade de sementes. Assim, o presente estudo teve como objetivo adequar a metodologia do teste de tetrazólio para a determinação da viabilidade de sementes de grumixameira, pitangueira e uvaieira. Sementes dessas três espécies foram pré-umedecidas em água por 3 h a 25 ºC. Após, foram coloridas com soluções de tetrazólio nas concentrações de 0,100%, 0,125% e 0,250% por 2, 3 e 6 horas, a 35 °C no escuro. Após lavagem, determinou-se a viabilidade das sementes. As sementes foram também submetidas a diferentes níveis de secagem e foram analisadas quanto aos seus atributos fisiológicos pelos testes de germinação e condutividade elétrica. O teste de tetrazólio, com coloração das sementes a 35 ºC usando-se uma concentração da solução de 0,250% com incubação por 3 horas para sementes de grumixameira, a 0,125% por 3 horas para sementes de pitangueira e a 0,100% por 2 horas para sementes de uvaieira, mostra-se como uma eficiente ferramenta para a avaliação da viabilidade dessas sementes.Termos para indexação: sementes florestais, sementes recalcitrantes, secagem, teste de viabilidade.

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Section: Resultssupporting

confidence: 45%

Section: Introductionmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Methodology of the tetrazolium test for assessing the viability of seeds of Eugenia brasiliensis Lam., Eugenia uniflora L. and Eugenia pyriformis Cambess

Lamarca

Barbedo

2014

J. Seed Sci.

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“…Bumelia obtusifolia (Nascimento, 2013), Plinia trunciflora (Hössel et al, 2013) y Anadenanthera peregrina (Pinho et al, 2011).…”

Section: Determinación De Patrones Topológicosmentioning

confidence: 99%

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Camacho

Tatis²,

Ayala³

2017

Mesoam. Agron.

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© 2017 Agronomía Mesoamericana es desarrollada en la Universidad de Costa Rica y se encuentra licenciada con Creative Commons Reconocimiento-NoComercial-SinObraDerivada 3.0 Costa Rica. Para más información escríbanos a pccmca@ucr.ac.cr ResumenEl objetivo del estudio fue describir las características morfométricas, anatómicas y la viabilidad de las semillas de Cedrela odorata y Cariniana pyriformis. El trabajo se llevó a cabo en la Universidad de Córdoba de Montería, Colombia, en el período comprendido entre abril de 2012 a julio de 2014; con una metodología descriptiva de laboratorio, la prueba de tetrazolio y la prueba de germinación en cámara germinativa. Se utilizaron semillas de lotes comerciales de tres localidades del departamento de Córdoba. Se seleccionaron al azar cinco árboles de cada especie y se tomaron al azar cinco muestras de cien semillas de cada uno. La descripción de la anatomía se hizo con base en diez semillas de cada especie. Para la prueba de tetrazolio, se estableció un experimento bajo un diseño completamente al azar, con seis tratamientos y cuatro repeticiones de veinticinco semillas. Los seis tratamientos se originaron al combinar las concentraciones de tetrazolio de 0,5, 1,0 y 1,5%, con dos y tres horas de inmersión en la solución. Las características externas de C. odorata presentaron menor variación que las de C. pyriformis, especialmente aquellas relacionadas con el peso. Cuatro patrones topológicos fueron identificados en las semillas de cada especie. Se pudo determinar la viabilidad para C. pyriformis y C. odorata, con inmersiones por dos horas en concentraciones de 0,5 y 1,0% de tetrazolio, respectivamente.Palabras clave: calidad de semilla, germinación de semillas, prueba de tetrazolio. AbstractThe aim of the study was to identify morphometric, anatomical, and the viability characteristics of the seeds of Cedrela odorata and Cariniana pyriformis. The study was carried out at the Universidad de Córdoba of Montería, Colombia, from April 2012 to July 2014; the study was carried out with a laboratory descriptive methodology, tetrazolium and germination tests in germination chamber were performed. Seeds of commercial lots from three locations in the department of Cordoba, were used. Five trees of each specie were randomly selected and from each tree, five samples of one hundred seeds, were randomly selected. The description of the shape and the anatomy of the seeds was made according to ten seeds of each specie. For the tetrazolium tests, a completely randomized design experiment, with six treatments, and four replications of 25 seeds, was established. The six treatments were originated

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Packing, moisture and environment for conservation of ‘jabuticatree açu’ seeds during storage

Hossel

Júnior

et al. 2018

Com. Sci.

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The objective of this study was to evaluate the viability of ‘jabuticaree açu’ [Plinia cauliflora (DC.) Berg] seeds according to water loss and packaging conservation conditions. Two experiments were carried out at UTFPR - Dois Vizinhos Campus, Parana State, Brazil. For the first experiment, the seeds were submitted to the hydro conditioning process by soaking in water during 24 hours with subsequently storage in a BOD chamber at 25°C for 0, 12, 24, 36, 48, 60, 72, 84 and 96 hours. This experiment was conducted in a completely randomized design with four replications of 50 seeds each. For the second experiment the seeds were separated into three lots: PET® bottles with lids, kraft® paper bags at room temperature and in cold storage (6°C±1°C). Each lot was stored during 0, 5, 10, 15, 20, 25, 30, 60, 90, 120 and 150 days. The experiment was carried out in a completely randomized design, in a 3x11 factorial (storage location x storage period), with four repetitions of 50 seeds each. Plinia cauliflora seeds present storage capacity of 96 hours at room temperature without loss of germination capacity, when previous hydro conditioning was carried out. Storage using Pet® bottles at room temperature for up to 25 days allows seeds viability.

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Conservação e teste de tetrazólio em sementes de jabuticabeira

Cited by 8 publications

References 6 publications

Methodology of the tetrazolium test for assessing the viability of seeds of Eugenia brasiliensis Lam., Eugenia uniflora L. and Eugenia pyriformis Cambess

Methodology of the tetrazolium test for assessing the viability of seeds of Eugenia brasiliensis Lam., Eugenia uniflora L. and Eugenia pyriformis Cambess

Características morfométricas, anatómicas y viabilidad de semillas de Cedrela odorata L. y Cariniana pyriformis Miers*.

Packing, moisture and environment for conservation of ‘jabuticatree açu’ seeds during storage

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