Conservation of biodiversity of highly important medicinal plants of India through tissue culture technology- A review

Sharma, Sudhir Kumar; Rathi, Neelima; Kamal, Barkha; Pundir, Dipika; Kaur, Baljinder; Arya, Sarita

doi:10.5251/abjna.2010.1.5.827.833

Cited by 63 publications

(33 citation statements)

References 18 publications

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“…Tylophora indica, an indigenous economically important threatened species (Sharma et al 2010) belonging to the family Asclepiadaceae has been included as an official drug in the Bengal pharmacoepia of 1884 (Gopalakrishnan et al 1980). It is traditionally used as a treatment for bronchial asthma, dysentery, rheumatism, dermatitis, inflammation and jaundice; as an expectorant; and as a treatment to induce emesis (Kirtikar and Basu 1991;Chopra et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular variation in Ri-transformed root lines are stable in long term cultures of Tylophora indica

2014

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Agrobacterium rhizogenes (Ri) transformed root lines of Tylophora indica were established and characterized on the basis of morphology, insertion and expression of T-DNA genes, DNA profiling and tylophorine content in order to study the stability of Ri-transformed root lines in long term culture. Morphologically Ri-transformed root lines were of four phenotypes-moderately branched thin roots, moderately branched thick roots, highly branched thin roots and highly branched thick roots. On the basis of the presence and expression of different T-DNA genes, the Ritransformed root lines could be divided into two groupsGroup I (13 %): TL ? /TR ? and Group II (87 %): TL ? /TR -. The presence and expression of TR-DNA in addition to TL-DNA did not have any effect on the root morphology and tylophorine content. Tylophorine content varied from 1.01 ± 0.05 to 1.25 ± 0.02 mg gDW -1 in 15 Ri-transformed root lines studied. The transformed root lines stably retained their characteristic phenotype, growth rate, integration and expression of T-DNA genes and tylophorine accumulation potential in long term culture i.e., for 4 years. None of the root lines showed any difference in DNA fingerprinting profiles for each of the 11 OPA primers, showing genetic stability and clonal fidelity of the root lines and root clones in long term culture. Transformed root lines A 4 20 and A 4 26 showing high tylophorine content (1.24 ± 0.03 and 1.22 ± 0.05 mg gDW -1 respectively) even after 4 years of maintenance in vitro on phytohormone unsupplemented medium can be used for scale up studies. This work endorses the utility of T. indica transformed roots for the production of secondary metabolites in bioreactors.

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Section: Introductionmentioning

confidence: 99%

Morphological and molecular variation in Ri-transformed root lines are stable in long term cultures of Tylophora indica

2014

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“…These natural products have also been using in pharmaceuticals, agrochemicals, ingredients of flavors and fragrances, food additives and pesticides (Sharma et al 2010). The natural habitats for medicinal plants are vanishing at a faster rate due to developmental activities of nation.…”

Section: Introductionmentioning

confidence: 99%

Comparative Microbicidal Efficacy of Fractionated Extracts from In Vitro and In Vivo Raised Cells of Tinosporacordifolia Against MDR Pathogens

Mittal

Singh

Batra

et al. 2016

Braz. arch. biol. technol.

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“…Numerous factors are reported to influence the success of in vitro propagation of medicinal plants. Some important reviews are the following: Rout et al 2000b;Nalawade et al 2003;Ozel et al 2006;Rajeswari and Paliwal 2006;Chaturvedi et al 2007;Neelofar et al 2007;Rath and Puhan 2009, Sasikumar et al 2009, Sharma et al 2010, Bawra et al 2010, Karaguzel et al 2012Sayanika et al 2012;Amin et al 2013). As a rule, these reviews cover a wide range of MAPs.…”

Section: Propagation By Shoot and Node Culturementioning

confidence: 99%

In Vitro Micropropagation of Medicinal and Aromatic Plants

Máthé

Hassan

Kader

2015

Medicinal and Aromatic Plants of the World

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Modern biotechnological methods like in vitro micropropagation technique hold tremendous potential for the production of high-quality plant-based medicine. They also allow to achieve the large scale multiplication of diseasefree plants, faster cloning and the conservation of desired genotypes, in a very short span of time. Via genetic transformation techniques, the modification of both genetic information of MAPs and the regulation of genes responsible for the production of valuable biologically active substances has also become possible in either higher amounts or with better properties.Micropropagation protocols are worked out for many plant species cultured in vitro to provide macro-and micro-mineral nutrients, vitamins, source of carbohydrates, appropriate environmental conditions (light intensity, photoperiod and temperature) and plant growth regulators required to obtain high regeneration rates. As such they are expected to facilitate commercially feasible micropropagation. Well-defined cell culture methods have also been developed to produce pharmacologically important secondary metabolites. Genetic engineering is applied to produce transgenic medicinal plants and metabolites.This chapter offers a brief insight into the present status of the biotechnology of MAPs. Special emphasis has been placed on the in vitro micropropagation and rapid clonal multiplication of selected elite genotypes, the regulation of organogenesis and somatic embryogenesis, the exploitation of advantages in somaclonal variation and genetic engineering techniques for both crop improvement and in vitro germplasm conservation. The production of flavor and volatile constituents in tissue cultures will also be briefly discussed, similarly to the application of farther biotechnological approaches.

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Conservation of biodiversity of highly important medicinal plants of India through tissue culture technology- A review

Cited by 63 publications

References 18 publications

Morphological and molecular variation in Ri-transformed root lines are stable in long term cultures of Tylophora indica

Morphological and molecular variation in Ri-transformed root lines are stable in long term cultures of Tylophora indica

Comparative Microbicidal Efficacy of Fractionated Extracts from In Vitro and In Vivo Raised Cells of Tinosporacordifolia Against MDR Pathogens

In Vitro Micropropagation of Medicinal and Aromatic Plants

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