Constituents of the Glandular Trichome Exudate on the Leaves of Laggera pterodonta

Liu, Bo; Wu, Ji‐Chun; Li, Haoliang; Ma, Yangmin; Yan-ping, Luo; Huang, Guoli; Tian, Xingjun; Chen, Ye‐Gao

doi:10.1007/s10600-016-1809-y

Cited by 5 publications

(1 citation statement)

References 13 publications

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“…There are also studies to investigate genetic control and regulation of biosynthesis of secondary metabolites in GTs. Some examples include terpenoid synthesis in type Ⅵ GTs of tomato ( Ewas et al 2016 ; Gong et al 2021 ; Yang et al 2021 ), artemisinin synthesis in GTs of sweet wormwood ( A. annua ) ( Shen et al 2016 ; Chen et al 2017 ; Hassani et al 2020 ), cannabinoid synthesis in GTs of cannabis ( Livingston et al 2020 ), and biosynthesis of metabolites in some medicinal plants ( Zhang et al 2015 ; Liu, Wu, et al 2016 ; Liu et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Novel players in organogenesis and flavonoid biosynthesis in cucumber glandular trichomes

et al. 2023

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Glandular trichomes (GTs) are outgrowths of plant epidermal cells that secrete and store specialized secondary metabolites that protect plants against biotic and abiotic stresses and have economic importance for human use. While extensive work has been done to understand the molecular mechanisms of trichome organogenesis in Arabidopsis (Arabidopsis thaliana), which forms unicellular, non-glandular trichomes (NGTs), little is known about the mechanisms of GT development or regulation of secondary metabolites in plants with multicellular GTs. Here, we identified and functionally characterized genes associated with GT organogenesis and secondary metabolism in GTs of cucumber (Cucumis sativus). We developed a method for effective separation and isolation of cucumber GTs and NGTs. Transcriptomic and metabolomic analyses showed that flavonoid accumulation in cucumber GTs is positively associated with increased expression of related biosynthesis genes. We identified 67 GT development-related genes, the functions of 7 of which were validated by virus-induced gene silencing. We further validated the role of cucumber ECERIFERUM1 (CsCER1) in GT organogenesis by overexpression and RNA interference transgenic approaches. We further show that the transcription factor TINY BRANCHED HAIR (CsTBH) serves as a central regulator of flavonoid biosynthesis in cucumber glandular trichomes. Work from this study provides insight into the development of secondary metabolite biosynthesis in multi-cellular glandular trichomes.

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