Constitutive expression of Arabidopsis DREB1B in transgenic potato enhances drought and freezing tolerance

Movahedi, Sara; Tabatabaei, B. E. Sayed; Alizade, H.; Ghobadi, Cyrus; Yamchi, Ahad; Khaksar, G.

doi:10.1007/s10535-012-0013-6

Cited by 51 publications

(17 citation statements)

References 35 publications

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“…AtDREB1 can improve the tolerance of Arabidopsis to drought, and AtDREB2 can increase the survival rate of Arabidopsis under cold stress (Liu et al, 1998). The factors have since been isolated and characterized in many plant species, showing that DREBs are involved in a variety of stress responses (Peng et al, 2011;Movahedi et al, 2012;Sun et al, 2013;Wang et al, 2013;Li et al, 2018;Jangale et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…CRPK-14-3-3s-CBF and CRLK-MEKK-MPK-ICE-CBF comprise the main signal transduction cascades (Shi et al, 2018b). C-repeatbinding factor (CBF) proteins recognize and bind to the conserved CRT/DRE cis-element (CCGAC) in the promoters of a subset of COR (cold-regulated) genes, inducing expression and enhancing freezing tolerance in plants (Yang et al, 2011;Movahedi et al, 2012;Guo et al, 2018;Shi et al, 2018b). MbDREB1, isolated from Malus baccata, can activate expression of COR15 and rd29B via ABA-independent and ABA-dependent pathways (Yang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Analysis of the DREB Subfamily in Saccharum spontaneum Reveals Their Functional Divergence During Cold and Drought Stresses

et al. 2020

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Drought and cold stresses are the main environmental factors that affect the yield of sugarcane, and DREB genes play very important roles in tolerance to drought, cold, and other environmental stresses. In this study, bioinformatics analysis was performed to characterize Saccharum spontaneum SsDREB genes. RNA sequencing (RNA-seq) was used to detect the expression profiles of SsDREBs induced by cold and drought stresses. According to our results, there are 110 SsDREB subfamily proteins in S. spontaneum, which can be classified into six groups; 106 of these genes are distributed among 29 chromosomes. Inter-and intraspecies synteny analyses suggested that all DREB groups have undergone gene duplication, highlighting the polyploid events that played an important role in the expansion of the DREB subfamily. Furthermore, RNA-seq results showed that 45 SsDREBs were up-or downregulated under cold stress; 35 of them were found to be involved in responding to drought stress. According to protein-protein interaction analysis, SsDREB100, SsDREB102, and SsDREB105 play key roles during the response to cold stress. These results reveal that functional divergence exists between collinear homologous genes or among common origin genes in the DREB subfamily of S. spontaneum. This study presents a comprehensive analysis and systematic understanding of the precise mechanism of SsDREBs in response to abiotic stress and will lead to improvements in sugarcane.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the DREB Subfamily in Saccharum spontaneum Reveals Their Functional Divergence During Cold and Drought Stresses

et al. 2020

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“…Many stress-related genes have been identified and characterized for their roles (Gelvin 2005;He et al 2010;Zhang et al 2010;Subramanyam et al 2011;Bhuiyan et al 2011;Li et al 2011;Movahedi et al 2012;Yarra et al 2012). The goal of our study was to investigate the potential of the salt stress tolerant candidate gene in major crop such as soybean.…”

Section: Discussionmentioning

confidence: 99%

“…The genetic transformation technique provides a powerful tool for overcoming abiotic stress of many species. (Gelvin 2005;Phang et al 2008;He et al 2010;Zhang et al 2010;Bhuiyan et al 2011;Li et al 2011;Subramanyam et al 2011;Movahedi et al 2012;Yarra et al 2012). Soybean transgenic plants with multiple functions have been developed using Agrobacterium-mediated transformation system based on the cotyledonary-node (CN) method (Hinchee et al 1988) and improved by using half-seed explants (Paz et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

Kim¹,

Kim²,

Pak³

et al. 2017

Plant Breed. Biotech

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Plants have adapted to environmental challenges by expressing many plant genes in response to the stresses. Among those genes, CCCH zinc finger proteins are involved in abiotic and biotic stresses. Transgenic soybean plants overexpressing AtSZF2 were produced to investigate that its ectopic overexpression enhanced salt stress tolerance by Agrobacterium-mediated transformation using half-seed explants. Sixteen transgenic lines were chosen to analyze for T-DNA insertion and transcription levels, and most of them were confirmed as positive. In further analysis with Southern blot, stable transformation event and copy number were confirmed. Following high salinity stress on the detached leaf and whole plant of two transgenic lines (#4 and #6) revealed that the ectopic expression of AtSZF2 was correlated with stress tolerance in phenotype, ion leakage and chlorophyll content with statistical significance. In another test with 20% PEG treatment, similar tolerance of transgenic plants was observed with lower ion leakage and higher chlorophyll content, indicating that the damage of cell membrane was prevented in transgenic plants. Finally, expression of various abiotic stress-responding genes was detected by reverse transcriptase and quantitative real-time PCR analysis with the transgenic plants. It could be proposed that introduction of AtSZF2 resulted in the modulation of ABA/stress responsive gene expression in transgenic soybean plants and make them tolerant against salt stress. Considering soybean as a salt-sensitive crop and importance of salt stress tolerance in specific farming region, the introduction of AtSZF2 may provide an approach for crop improvement in soybean breeding.

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“…Recently, drought tolerance of AtDREB1A-expressing potato plants was carried out in an in vitro study (Huynh et al, 2014), suggesting that overexpression of AtDREB1A enhances tolerance of potato against various environmental stresses. Belonging to the same AP2/ ERF family of transcription factors, overexpression of AtDREB1B in potato plants increased the accumulation of proline and preserved the relative water content, therefore leading to better overall plant growth under drought conditions (Movahedi et al, 2012). Arabidopsis DREB orthologues in potato also enhanced drought tolerance when overexpressed in transgenic potato plants.…”

Section: Transgenics Of Drought and Heat Tolerance In Potatomentioning

confidence: 97%

Recent advances in potato genomics, transcriptomics, and transgenicsunder drought and heat stresses: a review

Aksoy

Demirel²,

Öztürk³

et al. 2015

Turk J Bot

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Sustainable potato production practices are crucial for food security and social sustainability in the future since potato is a highly nutritious food and it is considered as one of the most promising crops to reduce human hunger and poverty in the world due to its high yield potential. However, being a temperate crop, potato is exposed to various environmental stresses, including extended periods of drought and heat. The majority of potato genomics, transcriptomics, and transgenics studies concentrate on the characterization of molecular mechanisms governing cold hardiness of tubers and response and tolerance mechanisms against diseases. Likewise, potato breeding studies focus on increasing the yield, extending the postharvest storage, and developing cultivars that withstand biotic stresses. The number of genomics, transcriptomics, and transgenics studies of drought and heat tolerance in potato is limited, although they are necessary state-of-the-art research procedures to characterize and identify the regulatory mechanism underlying any stresses in order to develop new crop varieties that can tolerate harsh environmental conditions. For these reasons, this review focuses on recent advances in genomics, transcriptomics, and transgenics of drought and heat tolerance in potato.

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Constitutive expression of Arabidopsis DREB1B in transgenic potato enhances drought and freezing tolerance

Cited by 51 publications

References 35 publications

Genome-Wide Analysis of the DREB Subfamily in Saccharum spontaneum Reveals Their Functional Divergence During Cold and Drought Stresses

Genome-Wide Analysis of the DREB Subfamily in Saccharum spontaneum Reveals Their Functional Divergence During Cold and Drought Stresses

Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

Recent advances in potato genomics, transcriptomics, and transgenicsunder drought and heat stresses: a review

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