“…The biosensing method has many advantages over these routine techniques in terms of fast response, simplicity, sensitivity and cost of determination (Shah, 1996). Various electrochemical methods have been proposed for xanthine analysis (Rehak et al, 1994;Shah, 1996;Pei and Li, 2000;Devi et al, 2011a). However, these electrochemical biosensors also had some problems like lack of stability, sensitivity, reusability, low reproducibility, slow electron transfer and leaking of enzyme due to non covalent immobilization, which are still to be improved.…”