Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft

Kuang, Haizhu; Wang, Yao; Shi, Yu; Yao, Wangchao; He, Xi; Liu, Xuezhe; Mo, Xiumei; Lu, Shuyang; Zhang, Peng

doi:10.1016/j.biomaterials.2020.120288

Cited by 71 publications

(44 citation statements)

References 42 publications

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“…A purpose-built electrospinning device (SS-3556H, Ucalery, Beijing, China) with a double-nozzle conjugated electrospinning system was utilized to fabricate two-dimensional PLCL/SF scaffolds (2DS) as described previously [26]. PLCL and SF were dissolved in HFIP in a mass ratio of 8:2 at a total concentration of 10 % (w/v).…”

Section: Fabrication Of Two-dimensional Nano Ber Matsmentioning

confidence: 99%

Chondroitin Sulphate and Gas Foaming Concurrently Improve Articular Cartilage Regeneration in Electrospun Poly(L-lactide-co-ε-caprolactone)/Silk Fibroin Based Scaffolds

Chen

Shafiq

et al. 2021

Preprint

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Degenerated cartilage tissues remain a burgeoning issue to be tackled, while bioactive engineering products available for optimal cartilage regeneration are scarce. In the present study, two-dimensional (2DS) poly(L-lactide-co-ε-caprolactone)/silk fibroin (PLCL/SF)-based scaffolds were fabricated by conjugate electrospinning method, and then cross-linked with chondroitin sulfate (CS) to further enhance their mechanical and biological performance. Afterwards, three-dimensional PLCL/SF scaffolds (3DS) and CS-crosslinked three-dimensional scaffolds (3DCSS) with tailored size were successfully fabricated by in situ gas foaming in a confined mold and subsequently freeze-dried. Gas-foamed scaffolds exhibited high porosity, rapid water absorption, and stable mechanical properties. While all of the scaffolds exhibited excellent cytocompatibility in vitro; 3DCSS showed better cell seeding efficiency and chondro-protective effect as compared to the other scaffolds. Histological analysis of chondrocytes-seeded constructs after cultivation for up to 6 weeks in vitro also confirmed that 3DCSS scaffolds supported the formation of cartilage-like tissues along with the more secretion of cartilage-specific extracellular matrix than that of the other groups. The reparative potential of 3DCSS was further evaluated in an articular cartilage defect model in rabbits, which exhibited a well-integrated boundary and attenuated inflammation demonstrating less expression of pro-inflammatory cytokines, such as interleukin (IL)-1β and tumor necrosis factor (TNF)-α. Taken together, the engineered biomimetic 3DCSS may provide a well-suited therapeutic option for cartilage tissue regeneration applications.

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Section: Fabrication Of Two-dimensional Nano Ber Matsmentioning

confidence: 99%

Chondroitin Sulphate and Gas Foaming Concurrently Improve Articular Cartilage Regeneration in Electrospun Poly(L-lactide-co-ε-caprolactone)/Silk Fibroin Based Scaffolds

Chen

Shafiq

et al. 2021

Preprint

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“…We have chosen the electrospinning technique for the dual loading of bioactive agents (ZnO and OEO). Poly ( l -lactide-co-caprolactone) (PLCL) is a synthetic polymer with excellent mechanical properties, biocompatibility as well as biodegradability [ 31 ]. Moreover, its potential as a drug carrier is also evident [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Multifunctional bioactive core-shell electrospun membrane capable to terminate inflammatory cycle and promote angiogenesis in diabetic wound

Khan

Huang

Shahriari-Khalaji

et al. 2021

Bioactive Materials

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Diabetic wound (DW) healing is a major clinical challenge due to multifactorial complications leading to prolonged inflammation. Electrospun nanofibrous (NF) membranes, due to special structural features, are promising biomaterials capable to promote DW healing through the delivery of active agents in a controlled manner. Herein, we report a multifunctional composite NF membrane loaded with ZnO nanoparticles (NP) and oregano essential oil (OEO), employing a new loading strategy, capable to sustainedly co-deliver bioactive agents. Physicochemical characterization revealed the successful fabrication of loaded nanofibers with strong in vitro anti-bacterial and anti-oxidant activities. Furthermore, in vivo wound healing confirmed the potential of bioactive NF membranes in epithelialization and granulation tissue formation. The angiogenesis was greatly prompted by the bioactive NF membranes through expression of vascular endothelial growth factor (VEGF). Moreover, the proposed NF membrane successfully terminated the inflammatory cycle by downregulating the pro-inflammatory cytokines interleukin −6 (IL-6) and matrix metalloproteinases-9 (MMP-9). In vitro and in vivo studies revealed the proposed NF membrane is a promising dressing material for the healing of DW.

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“…Among them, the PLLA blend with oligo-L-lactide-ε-caprolactone (OLCL) has an aliphatic chain, flexible character, and low melting temperature, which can improve the mechanical properties of PLLA [ 14 ]. Additionally, PLCL, with its great compliance matching and mechanical properties with natural blood vessels, has already been approved by the FDA for clinical applications [ 15 , 16 , 17 , 18 , 19 ]. The in vivo degraded acidic by-products of PLLA can contribute to a noninfectious inflammatory response in the body.…”

Section: Introductionmentioning

confidence: 99%

Poly(L-Lactic Acid) Composite with Surface-Modified Magnesium Hydroxide Nanoparticles by Biodegradable Oligomer for Augmented Mechanical and Biological Properties

et al. 2021

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Poly(L-lactic acid) (PLLA) has attracted a great deal of attention for its use in biomedical materials such as biodegradable vascular scaffolds due to its high biocompatibility. However, its inherent brittleness and inflammatory responses by acidic by-products of PLLA limit its application in biomedical materials. Magnesium hydroxide (MH) has drawn attention as a potential additive since it has a neutralizing effect. Despite the advantages of MH, the MH can be easily agglomerated, resulting in poor dispersion in the polymer matrix. To overcome this problem, oligo-L-lactide-ε-caprolactone (OLCL) as a flexible character was grafted onto the surface of MH nanoparticles due to its acid-neutralizing effect and was added to the PLLA to obtain PLLA/MH composites. The pH neutralization effect of MH was maintained after surface modification. In an in vitro cell experiment, the PLLA/MH composites including OLCL-grafted MH exhibited lower platelet adhesion, cytotoxicity, and inflammatory responses better than those of the control group. Taken together, these results prove that PLLA/MH composites including OLCL-grafted MH show excellent augmented mechanical and biological properties. This technology can be applied to biomedical materials for vascular devices such as biodegradable vascular scaffolds.

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Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft

Cited by 71 publications

References 42 publications

Chondroitin Sulphate and Gas Foaming Concurrently Improve Articular Cartilage Regeneration in Electrospun Poly(L-lactide-co-ε-caprolactone)/Silk Fibroin Based Scaffolds

Chondroitin Sulphate and Gas Foaming Concurrently Improve Articular Cartilage Regeneration in Electrospun Poly(L-lactide-co-ε-caprolactone)/Silk Fibroin Based Scaffolds

Multifunctional bioactive core-shell electrospun membrane capable to terminate inflammatory cycle and promote angiogenesis in diabetic wound

Poly(L-Lactic Acid) Composite with Surface-Modified Magnesium Hydroxide Nanoparticles by Biodegradable Oligomer for Augmented Mechanical and Biological Properties

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