Construction of a BAC Library for a Defoliating Insect-Resistant Soybean and Identification of Candidate Clones Using a Novel Approach

Zhu, Shushuai; Saski, Chris; Boerma, H. R.; Tomkins, Jeffrey; All, John; Parrott, W. A.

doi:10.1007/s11105-008-0077-9

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…An advantage of BAC libraries is that they allow the assembly of complex genomes where levels of heterozygosity and repetitive DNA are difficult to accurately reconstruct with a whole‐genome shotgun (wgs) approach leaving assemblies highly fragmented and unordered. BACs facilitate prioritized subgenomic sequencing and localized assemblies (Adams et al ., ; Holt et al ., ), the positional cloning and the identification of genomic regions of interest (Tomkins et al ., ; Zhu et al ., ), and the generation of BAC‐based physical genome maps (Yamamoto et al ., ; Aggarwal et al ., ). Recently, after screening a fosmid library, we were able to show that two genes encoding active xylanases resembling bacterial‐derived genes were in fact endogenous to the genome of the mustard leaf beetle, Phaedon cochleariae , and were most likely acquired by the beetle through horizontal gene transfer from a species of gammaproteobacteria (Pauchet & Heckel, ).…”

Section: Introductionmentioning

confidence: 99%

Studying the organization of genes encoding plant cell wall degrading enzymes inChrysomela tremulaprovides insights into a leaf beetle genome

Pauchet

Saski

Feltus

et al. 2014

Insect Molecular Biology

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The ability of herbivorous beetles from the superfamilies Chrysomeloidea and Curculionoidea to degrade plant cell wall polysaccharides has only recently begun to be appreciated. The presence of plant cell wall degrading enzymes (PCWDEs) in the beetle's digestive tract makes this degradation possible. Sequences encoding these beetle-derived PCWDEs were originally identified from transcriptomes and strikingly resemble those of saprophytic and phytopathogenic microorganisms, raising questions about their origin; e.g. are they insect- or microorganism-derived? To demonstrate unambiguously that the genes encoding PCWDEs found in beetle transcriptomes are indeed of insect origin, we generated a bacterial artificial chromosome library from the genome of the leaf beetle Chrysomela tremula, containing 18 432 clones with an average size of 143 kb. After hybridizing this library with probes derived from 12 C. tremula PCWDE-encoding genes and sequencing the positive clones, we demonstrated that the latter genes are encoded by the insect's genome and are surrounded by genes possessing orthologues in the genome of Tribolium castaneum as well as in three other beetle genomes. Our analyses showed that although the level of overall synteny between C. tremula and T. castaneum seems high, the degree of microsynteny between both species is relatively low, in contrast to the more closely related Colorado potato beetle.

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Section: Introductionmentioning

confidence: 99%

Studying the organization of genes encoding plant cell wall degrading enzymes inChrysomela tremulaprovides insights into a leaf beetle genome

Pauchet

Saski

Feltus

et al. 2014

Insect Molecular Biology

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“…The insect resistance sources PI 229358 (MG VII) and PI 227687 (MG VIII) are Japanese cultivars known as ‘Soden‐daizu’ and ‘Miyako White’, respectively (USDA‐ARS, 2015). Simple sequence repeat (SSR) markers were used during backcross and selfing generations to select lines carrying a particular QTL combination: Sat_258 and Satt702 for QTL‐M (Zhu et al, 2009), Sct_199 and Satt191, for QTL‐G (Zhu et al, 2008), Sat_334 and Sat_122, for QTL‐H (Zhu et al, 2008), and Sat_112 and Satt411 for QTL‐E (Hulburt, 2002). Primer sequences for the SSR markers were obtained from SoyBase (http://www.soybase.org) (Grant et al, 2010).…”

Section: Methodsmentioning

confidence: 99%

Registration of Two Soybean Germplasm Lines Containing Leaf‐Chewing Insect Resistance QTLs from PI 229358 and PI 227687 Introgressed into ‘Benning’

Ortega

Lail

Wood

et al. 2017

J of Plant Registrations

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Two soybean [Glycine max (L.) Merr.] germplasm lines, Benning-ME (Reg. No. GP-405, PI 679958) and Benning-MGHE (Reg. No. GP-406, PI 679959), were developed by the University of Georgia Agricultural Experiment Stations.Control of insect pests is crucial in soybean production; hostplant resistance reduces the need for insecticide applications, thus diminishing production costs and pesticide concerns. In soybean, resistance to a broad range of leaf-chewing insects is found in the Japanese accessions PI 229358 and PI 227687. In PI 229358, resistance is conferred by quantitative trait loci (QTLs) M, H, and G. In PI 227687, resistance is conferred by QTL-E. To enhance soybean resistance to leafchewing insects, QTLs of PI 229358 and PI 227687 were pyramided in Benning-ME and Benning-MGHE, which are near-isogenic lines of 'Benning' obtained through markerassisted backcrossing. Under ield conditions, Benning-ME and Benning-MGHE sustain 67 and 57% less defoliation than Benning, respectively. To determine the QTL introgressions in each line, high-density single-nucleotide polymorphism (SNP) genotypes were obtained using the SoySNP50K iSelect BeadChip (Illumina). To facilitate selection of lines carrying a speciic QTL pyramid, Kompetitive Allele Speciic Polymerase Chain Reaction markers were developed for high-throughput genotyping. These lines are valuable genetic resources for breeding of host-plant resistance to insects in soybean. The combination of QTL-M and QTL-E provides agriculturally relevant levels of resistance, and with only two loci, the use of this pyramid is feasible in a breeding program.

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“…Simple sequence repeat (SSR) markers linked to each QTL were used during backcross and selfing generations to select lines carrying a specific QTL combination. The flanking markers were: Sat_258 (5′-GCGCAATAGATAATCGAAAAACATACAAGA-3′ and 5′-GCGGGGAAAGTGAAAACAAGATCAAATA-3′) and Satt702 (5′-GCGGGGTTCTGTGGCTTCAAC-3′ and 5′-GCGCATTGGAATAACGTCAAA-3′) for QTL-M (Zhu et al 2009 ); Sct_199 (5′-GCGACAATGGCTATTAGTAACAATCA-3′ and 5′-GCGATTTTCTATTTTCCTCACAGTG-3′) and Satt191 (5′-CGCGATCATGTCTCTG-3′ and 5′-GGGAGTTGGTGTTTTCTTGTG-3′) for QTL-G (Zhu et al 2008 ); Sat_334 (5′-GCGTAACGTAGCAAATTGACTATAAGA-3′ and 5′-GCGTGTGCAAAGACAATTTCAATGA-3′) and Sat_122 (5′-GTGACAAATGGATGGACAATAG-3′ and 5′-AAGAAAAATAAAATAATGTAGAGTGGTGAT-3′) for QTL-H (Zhu et al 2008 ); and Sat_112 (5′-TGTACAGTATACCGACATAATA-3′ and 5′-CTACAAATAACATGAAATATAAGAAATA-3′) and Satt411 (5′-TGGCCATGTCAAACCATAACAACA-3′ and 5′-GCGTTGAAGCCGCCTACAAATATAAT-3′) for QTL-E (Hulburt 2002 ). Primer sequences for the SSR markers were obtained from SoyBase ( http://www.soybase.org ) (Grant et al 2010 ).…”

Section: Methodsmentioning

confidence: 99%

Pyramids of QTLs enhance host–plant resistance and Bt-mediated resistance to leaf-chewing insects in soybean

et al. 2016

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Key messageQTL-M and QTL-E enhance soybean resistance to insects. Pyramiding these QTLs withcry1Ac increases protection against Bt-tolerant pests, presenting an opportunity to effectively deploy Bt with host–plant resistance genes.AbstractPlant resistance to leaf-chewing insects minimizes the need for insecticide applications, reducing crop production costs and pesticide concerns. In soybean [Glycine max (L.) Merr.], resistance to a broad range of leaf-chewing insects is found in PI 229358 and PI 227687. PI 229358’s resistance is conferred by three quantitative trait loci (QTLs): M, G, and H. PI 227687’s resistance is conferred by QTL-E. The letters indicate the soybean Linkage groups (LGs) on which the QTLs are located. This study aimed to determine if pyramiding PI 229358 and PI 227687 QTLs would enhance soybean resistance to leaf-chewing insects, and if pyramiding these QTLs with Bt (cry1Ac) enhances resistance against Bt-tolerant pests. The near-isogenic lines (NILs): BenningME, BenningMGHE, and BenningME+cry1Ac were developed. BenningME and BenningMGHE were evaluated in detached-leaf and greenhouse assays with soybean looper [SBL, Chrysodeixis includens (Walker)], corn earworm [CEW, Helicoverpa zea (Boddie)], fall armyworm [FAW, Spodoptera frugiperda (J.E. Smith)], and velvetbean caterpillar [VBC, Anticarsia gemmatalis (Hübner)]; and in field-cage assays with SBL. BenningME+cry1Ac was tested in detached-leaf assays against SBL, VBC, and Southern armyworm [SAW, Spodoptera eridania (Cramer)]. In the detached-leaf assay, BenningME showed the strongest antibiosis against CEW, FAW, and VBC. In field-cage conditions, BenningME and BenningMGHE suffered 61 % less defoliation than Benning. BenningME+cry1Ac was more resistant than BenningME and Benningcry1Ac against SBL and SAW. Agriculturally relevant levels of resistance in soybean can be achieved with just two loci, QTL-M and QTL-E. ME+cry1Ac could present an opportunity to protect the durability of Bt genes in elite soybean cultivars. These results should assist the development of effective pest management strategies, and sustainable deployment of Bt genes in soybean.

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Construction of a BAC Library for a Defoliating Insect-Resistant Soybean and Identification of Candidate Clones Using a Novel Approach

Cited by 9 publications

References 31 publications

Studying the organization of genes encoding plant cell wall degrading enzymes inChrysomela tremulaprovides insights into a leaf beetle genome

Studying the organization of genes encoding plant cell wall degrading enzymes inChrysomela tremulaprovides insights into a leaf beetle genome

Registration of Two Soybean Germplasm Lines Containing Leaf‐Chewing Insect Resistance QTLs from PI 229358 and PI 227687 Introgressed into ‘Benning’

Pyramids of QTLs enhance host–plant resistance and Bt-mediated resistance to leaf-chewing insects in soybean

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