Construction of a gE-Deleted Pseudorabies Virus and Its Efficacy to the New-Emerging Variant PRV Challenge in the Form of Killed Vaccine

Wang, Tongyan; Xiao, Yan; Yang, Qingyuan; Wang, Yuzhou; Sun, Zhe; Zhang, Chaoling; Yan, Shijun; Wang, Juan; Guo, Linghua; He, Ying; Gao, Zhiyu; Wang, Lilin; Li, Xiangdong; Tan, Feifei; Tian, Kegong

doi:10.1155/2015/684945

Cited by 28 publications

(25 citation statements)

References 10 publications

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“…4a). However, unlike the PRV killed vaccine previously reported [17] , the gB antibody titers of vaccinated pigs dropped with average OD 405 of 2.0 after PRV HN1201 challenge. Although this subunit vaccine may not induce gB-specific antibodies lasting as long as the killed vaccine did, the high titer of gB antibodies were still enough to provide useful protection to PRV HN1201 challenge.…”

Section: Discussioncontrasting

confidence: 76%

“…In contrast, no vaccinated pigs showed any clinical symptoms except a transient fever. As found with the killed PRV vaccine [17] , this fever in vaccinated groups lasted for 4 days when body temperature returned to normal. In contrast, the unvaccinated pigs had lasting high fever until they died.…”

Section: Discussionsupporting

confidence: 66%

“…The tonsil, lung, brain, cerebellum and trigeminal samples were collected for hematoxylin and eosin staining with a Leica fully automatic dyeing machine according to standard procedures, and immunohistochemistry staining using previously described methods [17] . The slides were photographed at 400Â magnification.…”

Section: Histopathology and Immunohistochemistry Stainingmentioning

confidence: 99%

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Research and development of a novel subunit vaccine for the currently circulating pseudorabies virus variant in China

Wang¹,

Wang²,

He³

et al. 2015

Front. Agr. Sci. Eng.

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Pseudorabies (PR) is a devastating viral disease which leads to fatal encephalitis and respiratory disorders in pigs. Commercial gE-deleted live pseudorabies virus (PRV) vaccine has been widely used to control this disease in China. However, the new-emerging variants of PRV compromises the protection provided by current vaccines and lead to the outbreak of PR in vaccinated pig herds. Several killed and live vaccine candidates based on current PRV variants have been reported to be effective to control the disease. A subunit vaccine based on gB protein, one major PRV glycoprotein which elicits strong humoral and cellular immune responses, however, was never evaluated for protection against the current circulating PRV variants. In this study, full-length PRV gB protein was successfully expressed in baculovirus/insect cells in the soluble format and was tested on 3-week-old piglets as a subunit vaccine. Compared with unvaccinated pigs, the gB-vaccinated pigs developed specific antibody-mediated responses and were protected from the virulent PRV HN1201 challenge. All vaccinated pigs survived without showing any PRV-specific respiratory and neurological signs, but all unvaccinated pigs died within 7 days after HN1201 challenge. Hence, this novel gB-based vaccine could be applied as an effective subunit vaccine to control PRV variant in China.

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Section: Discussioncontrasting

confidence: 76%

Section: Discussionsupporting

confidence: 66%

Section: Histopathology and Immunohistochemistry Stainingmentioning

confidence: 99%

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Research and development of a novel subunit vaccine for the currently circulating pseudorabies virus variant in China

Wang¹,

Wang²,

He³

et al. 2015

Front. Agr. Sci. Eng.

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“…The H&E staining was operated automatically by a Leica fully automatic dyeing machine according to standard procedures. The immunohistochemistry staining was performed as previously described [9] . Photographs of the slides were taken at 400 Â magnification.…”

Section: Histopathology and Immunohistochemistry Stainingmentioning

confidence: 99%

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

Yan¹,

He²,

Zhang³

et al. 2016

Front. Agr. Sci. Eng.

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As virulence-determining genes, RR1 and RR2 encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted RR1 and RR2 genes based on a TK/gE/gI triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size in vitro as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

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“…Though some novel vaccines have been demonstrated to provide effective protection against the current PrV infections, these candidate vaccines are still under preclinical evaluation [13][14][15], emphasizing the demand for improvement of classical vaccines.…”

Section: Introductionmentioning

confidence: 99%

A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice

Wang

Cui

et al. 2020

Journal of Immunology Research

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Pseudorabies is an important infectious disease of swine, and immunization using attenuated pseudorabies virus (aPrV) vaccine is a routine practice to control this disease in swine herds. This study was to evaluate a saline solution containing ginseng stem-leaf saponins (GSLS) and sodium selenite (Se) as a vaccine adjuvant for its enhancement of immune response to aPrV vaccine. The results showed that aPrV vaccine diluted with saline containing GSLS-Se (aP-GSe) induced significantly higher immune responses than that of the vaccine diluted with saline alone (aP-S). The aP-GSe promoted higher production of gB-specific IgG, IgG1, and IgG2a, neutralizing antibody titers, secretion of Th1-type (IFN-γ, IL-2, IL-12), and Th2-type (IL-4, IL-6, IL-10) cytokines, and upregulated the T-bet/GATA-3 mRNA expression when compared to aP-S. In addition, cytolytic activity of NK cells, lymphocyte proliferation, and CD4 + /CD8 + ratio was also significantly increased by aP-GSe. More importantly, aP-GSe conferred a much higher resistance of mice to a field virulent pseudorabies virus (fPrV) challenge. As the present study was conducted in mice, further study is required to evaluate the aP-GSe to improve the vaccination against PrV in swine.

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Construction of a gE-Deleted Pseudorabies Virus and Its Efficacy to the New-Emerging Variant PRV Challenge in the Form of Killed Vaccine

Cited by 28 publications

References 10 publications

Research and development of a novel subunit vaccine for the currently circulating pseudorabies virus variant in China

Research and development of a novel subunit vaccine for the currently circulating pseudorabies virus variant in China

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice

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