Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations

Adamala, Katarzyna P.; Engelhart, Aaron E.; Kamat, Neha P.; Lin, Jin; Szostak, Jack W.

doi:10.1038/nprot.2015.054

Cited by 15 publications

(9 citation statements)

References 22 publications

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“…A large volume of O-solution is required, and the required long time (hours) restricts this purification method to those solutes that are not massively released from GVs in the allotted time, otherwise the intravesicle solute concentration would also decrease. Recently, the use of home-modified small-volume commercial dialysis cassettes for exchanging the solution outside vesicles has been reported [ 26 ]. This method is particularly useful when high-cost solutions must be exchanged with vesicle suspensions.…”

Section: Discussionmentioning

confidence: 99%

Rapid purification of giant lipid vesicles by microfiltration

et al. 2018

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Giant lipid vesicles (GVs) are emerging models for investigating the properties and reactivity of cell-like microcompartments, providing useful information about plausible protocellular structures in primitive times, as well as for the modern synthetic biology goal of constructing the first artificial cell from its reconstituted and partly modified components. Here we explore a novel methodology of GV purification by microfiltration under reduced pressure, operated by a simple apparatus. The method has been characterized in terms of flow rate, amount of lipid loss, quality of recovered GVs, and size distribution. A case study is reported to show the practicability of GV microfiltration. A clickable fluorescent probe was encapsulated inside GVs; more than 99.9% of the non-entrapped probe was easily and rapidly removed by multiple microfiltrations. This novel methodology is briefly discussed as a future tool for selection experiments on GV populations.

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Section: Discussionmentioning

confidence: 99%

Rapid purification of giant lipid vesicles by microfiltration

et al. 2018

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“…Liposomes are lipid bi-layer with outer hydrophobic and center hydrophilic compartments (Adamala et al, 2015). These nano-sized active molecules entangled between the hydrophobic membrane and hydrophilic position (Fig.…”

Section: Nanoparticles Formulationmentioning

confidence: 99%

Phytochemicals with nanoparticles and their potential applications in medicine – A review

Rajith-Varman¹,

Baskar²,

Kim³

et al. 2021

JEB

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Phytochemicals having multiple properties which are used to cure various diseases such as cancer, diabetes, neurodegeneration, angiogenesis, aging, inflammatory, cardiovascular and arthritic disease, etc., Extensive use of phytochemicals and their derivatives leads to unavailability or scarcity of products in nature. Despite this episode, the nanotechnology resolves the setback of handling phytochemicals by increasing their bioavailability, long-lost acting, target-specific and less or no toxicity. Here, we focused on the 24 known phytochemicals and preparation of NPs without discriminating the polymers and metals in NPs. All the phytochemicals did not fully satisfy the toxicological hazards, physical-pharmaceutical challenges, bioethical and health issues research. Hence these NPs and their NP incorporated phytochemicals need to be characterized in a way to give direct usage to the common man. Commercialization of NP formulations in pharmaceuticals needs statutory regulation norms for complete satisfaction and safety for end-users. As of now, many NP mediated pharmaceutical products are available in the market with full-fledged but still many unrevealed about their fate in the environment. In this review, the characterization of nanoparticles, the transparent benefit of nanoparticles (NPs), and their current application in various fields has been discussed.

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“…Start the timer and keep the reaction sample tumbling all the time. (Optional) For continuous fresh monomer feeding, transfer 300 µL of reaction solution to one chamber of a lab constructed small-volume liposome dialyzer 11 and put 300 µL feeding solution in the other chamber. The feeding solution should contain all the ingredients at the same concentration as in the reaction solution, except for replacing RNA containing vesicles with empty vesicles.…”

Section: Protocolmentioning

confidence: 99%

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Lin¹,

Engelhart²,

Adamala³

et al. 2018

JoVE

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Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of these amphiphiles. In particular, fatty acid liposomes enhance dynamic character, due to the relatively high solubility of single-chain amphiphiles. Similarly, liposomes containing free fatty acids are more sensitive to salt and divalent cations, due to the strong interactions between the carboxylic acid head groups and metal ions. Here we illustrate techniques for preparation, purification, and use of liposomes comprised in part or whole of single chain amphiphiles (e.g., oleic acids).

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Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations

Cited by 15 publications

References 22 publications

Rapid purification of giant lipid vesicles by microfiltration

Rapid purification of giant lipid vesicles by microfiltration

Phytochemicals with nanoparticles and their potential applications in medicine – A review

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

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