Construction of an infectious full-length cDNA clone of Chrysanthemum virus B

Ohkawa, A.; Ishikawa-Suehiro, Noriko; Okuda, Shigenobu; Natsuaki, Tomohide

doi:10.1007/s10327-008-0120-6

Cited by 8 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these, the full-length genome sequence of Japanese isolate (CVB-S) was the first to be obtained [ 11 ]. Subsequently, an infectious cDNA clone of CVB-S, pCVB, was constructed; however, no visible symptoms were observed in chrysanthemum seedlings infiltrated with pCVB [ 19 ]. In 2012, complete genome sequences of CVB-TN, CVB-PB, CVB-UP, and CVB-UK isolates from India were reported [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Zhong

Yang

et al. 2022

Virol J

View full text Add to dashboard Cite

Background Chrysanthemum virus B (CVB), a key member of the genus Carlavirus, family Betaflexiviridae, causes severe viral diseases in chrysanthemum (Chrysanthemum morifolium) plants worldwide. However, information on the mechanisms underlying the response of chrysanthemum plants to CVB is scant. Methods Here, an integrated next-generation sequencing and comparative transcriptomic analysis of chrysanthemum leaves was conducted to explore the molecular response mechanisms of plants to a Chinese isolate of CVB (CVB-CN) at the molecular level. Results In total, 4934 significant differentially expressed genes (SDEGs) were identified to respond to CVB-CN, of which 4097 were upregulated and 837 were downregulated. Gene ontology and functional classification showed that the majority of upregulated SDEGs were categorized into gene cohorts involved in plant hormone signal transduction, phenylpropanoid and flavonoid biosynthesis, and ribosome metabolism. Enrichment analysis demonstrated that ethylene pathway-related genes were significantly upregulated following CVB-CN infection, indicating a strong promotion of ethylene biosynthesis and signaling. Furthermore, disruption of the ethylene pathway in Nicotiana benthamiana, a model plant, using virus-induced gene silencing technology rendered them more susceptible to cysteine-rich protein of CVB-CN induced hypersensitive response, suggesting a crucial role of this pathway in response to CVB-CN infection. Conclusion This study provides evidence that ethylene pathway has an essential role of plant in response to CVB and offers valuable insights into the defense mechanisms of chrysanthemum against Carlavirus.

show abstract

Section: Introductionmentioning

confidence: 99%

Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Zhong

Yang

et al. 2022

Virol J

View full text Add to dashboard Cite

show abstract

“…Most commercial chrysanthemum cultivars are usually infected with CVB without showing visible symptoms. Chrysanthemum plants infected with CVB show different symptoms depending on the cultivar, such as mosaic, mottling, vein clearing, chlorosis, necrotic streaks, malformation and corrugation of the leaf blade (Hollings, 1957;Lin et al, 2005;Ohkawa et al, 2008). CVB, formerly known as chrysanthemum mild mosaic virus, is a member of the genus Carlavirus in the family Betaflexiviridae (Singh et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

First characterisation of chrysanthemum virus B infecting chrysanthemum in Thailand and development of colourimetric RT-LAMP for rapid and sensitive detection

Supakitthanakorn

Mochizuki

Vichittragoontavorn

et al. 2022

Folia Horticulturae

View full text Add to dashboard Cite

Chrysanthemum is among the world's most important ornamental plants because of its high economic and cultural value. Our report is the first to describe the detection of chrysanthemum virus B (CVB) in chrysanthemum leaf samples collected from Thailand, which showed yellowing and mild mottling symptoms. The coat protein sequences of CVB isolated in this study share 95.15% identity with previously characterised CVB isolates. Biological indexing found that CVB induced both local and systemic symptoms in tobacco plants, while petunia displayed systemic symptoms. To improve the rapidity and sensitivity of CVB detection, the loop-mediated isothermal amplification (LAMP) technique was developed. LAMP detection was found to be optimal when incubation was conducted at 65 °C for 45 min, wherein the LAMP reaction demonstrated 106 times higher sensitivity than polymerase chain reaction. To simplify the interpretation of results, we designed the method such that a positive result is clearly indicated based on a change of colour (colourimetry), from pink to yellow, as observed visually and via gel electrophoresis. To our best knowledge, this is the first report on the characterisation of molecular, biological and morphological characteristics of CVB infecting chrysanthemum in Thailand, along with the development of colourimetric RT-LAMP for improving detection efficiency.

show abstract

“…CVB is globally distributed where chrysanthemums are grown and most commercial cultivars are usually infected by CVB without showing visible symptoms, but some cultivars showed mild mosaic, vein clearing or necrotic streak symptoms on leaves and the owers, sometimes, are malformed [3][6] [12]. The transmission of this virus occurs by some aphids consisting Myzus persicae and Aphis gossypii as a non-persistent manner and is also sap-transmissible [13].…”

Section: Introductionmentioning

confidence: 99%

First Detection and Characterization of Chrysanthemum virus B infecting Chrysanthemum in Thailand

Supakitthanakorn

Wichitrakoonthavorn²,

Kunasakdakul

et al. 2021

Preprint

View full text Add to dashboard Cite

Chrysanthemum is one of the important ornamental plants in worldwide due to its high economic and cultural values. Chrysanthemum leaves showed mosaic, ringspot, yellowing and mild mottle symptoms were observed and collected from cultivation areas in northern Thailand and used for detection of important viruses infecting chrysanthemum. Chrysanthemum virus B (CVB) was detected by reverse transcription polymerase chain reaction (RT-PCR) from samples showing yellowing and mild mottle symptoms. Sequences of the coat protein (CP) gene of two CVB isolates found in this study were sequenced and shared 93.15% homology with other CVB isolates from different countries deposited in GenBank. Biological indexing of these CVB found that they induced both local and systemic symptoms in tobacco plants while petunia displayed a systemic symptom. The particles of CVB were observed under transmission electron microscope (TEM), prepared by dip preparation and negative staining methods, showing slightly flexuous rod-shaped virions approximately 600–650 nm in length. To our knowledge, this is the first detection and study on molecular and biological characteristics of CVB infecting chrysanthemum in Thailand.

show abstract

Construction of an infectious full-length cDNA clone of Chrysanthemum virus B

Cited by 8 publications

References 6 publications

Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

First characterisation of chrysanthemum virus B infecting chrysanthemum in Thailand and development of colourimetric RT-LAMP for rapid and sensitive detection

First Detection and Characterization of Chrysanthemum virus B infecting Chrysanthemum in Thailand

Contact Info

Product

Resources

About