Construction of castor functional markers fingerprint and analysis
of genetic diversity

Yang, Ting; Lü, Jing; Yeboah, Akwasi; Gu, Shuailei; Li, Dongna; Shi, Yuzhen; Yin, Xiaochen

doi:10.32604/biocell.2020.09112

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…Therefore, castor oil can be used for the production of biofuel in the chemical industry [7,8]. The demand for castor products such as castor oil and its derivatives has increased worldwide since 1991 [9][10][11]. After 2014, the import rate of castor raw materials in China has increased over 90% [10,12].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Castor Bean (Ricinus communis L.) Revealed by ISSR and RAPD Markers

et al. 2021

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Castor (Ricinus communis L.), known as castor oil plant or castor bean, is a non-edible oilseed crop. In the present study, the genetic diversity among 54 samples (3 wild and 51 cultivated) collected worldwide was evaluated using inter-simple sequence repeats (ISSRs) and random amplified polymorphic DNA (RAPD) markers. A total of 9 ISSR primers produced 83 high-resolution bands with 61 (74.53%) as polymorphic. The percentage of polymorphic bands per primer and the genetic similarity coefficient ranged from 54.55% (UBC-836) to 100% (UBC-808) and from 0.74 to 0.96, respectively. A total of 11 out of 20 RAPD primers amplified unique polymorphic products with an average percentage of polymorphic bands of 60.98% (56 polymorphic bands out of a total of 90 bands obtained). The percentage of polymorphic bands per primer ranged from 25% (OPA-02 and B7) to 90.91% (B21) with the genetic similarity coefficient ranging from 0.73 to 0.98. The unweighted pair group method with arithmetic averages (UPGMA) dendrogram using two molecular markers divided 54 castor genotypes into three groups. Furthermore, based on morphological data, all 54 castor varieties were grouped into three main clusters. The genetic diversity analysis based on two molecular makers showed that most varieties from China were closely related to each other with three varieties (GUANGDONGwild, ZHEJIANGWild, and HANNANWild) belonging to a wild group separated from most of the cultivated castor samples from China, India, France, and Jordan. These results suggested that the cultivated castor contains a narrow genetic base. Accordingly, we recommend that wild castor genetic resources be introduced for breeding novel castor varieties. Furthermore, the Vietnam, Malaysia, Indonesia, and Nigeria accessions were clustered into the same group. The results of principal coordinate analysis (PCoA) and UPGMA cluster analysis were consistent with each other. The findings of this study are important for future breeding studies of castor.

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Section: Introductionmentioning

confidence: 99%

“…The demand for castor products such as castor oil and its derivatives has increased worldwide since 1991 [9][10][11]. After 2014, the import rate of castor raw materials in China has increased over 90% [10,12]. To date, many methods or techniques have already been used to improve breeding and productivity of castor [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Castor Bean (Ricinus communis L.) Revealed by ISSR and RAPD Markers

et al. 2021

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Modern day breeding approaches for improvement of castor

Patel,

Menon,

Kumar

et al. 2024

Heliyon

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Genome-wide association study of the loci and candidate genes associated with agronomic traits in Amomum villosum Lour

Li,

Luo,

Shi

et al. 2024

PLoS ONE

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Amomum villosum Lour. (A. villosum) is a valuable herbaceous plant that produces the famous traditional Chinese medicine Amori Fructus. Identifying molecular markers associated with the growth of A. villosum can facilitate molecular marker-assisted breeding of the plant. This study employed 75 A. villosum accessions as the test material and utilized 71 pairs of polymorphic simple sequence repeat (SSR) molecular markers to genotype the population. The study analyzed the association between SSR markers and phenotypic traits through the linkage imbalance and population structure analysis. Candidate genes associated with the molecular markers were also identified. The results showed that the phenotypic diversity index range of the 12 agronomic traits was 4.081–4.312 and conformed to a normal distribution. Moreover, 293 allelic variations were detected in the 75 accessions, with an average of 5.32 amplified alleles per loci, ranging from 3 to 8. The maximum number of amplified alleles for AVL12 was 8. The population structure and cluster analysis indicated that the accessions could be divided into two subgroups. Using the mixed linear model (MLM) model of population structure (Q)+kinship matrix (K) for association analysis, three SSR molecular markers significantly associated with the agronomic traits were detected. Fluorescence quantification was used to analyze the expression levels of six candidate genes, and it was found that three of the genes were differentially expressed in phenotypically different accessions. This study is the first to use SSR markers for genome-wide association study (GWAS) mapping and identification of the associated agronomic traits in A. villosum. The results of this study provide a basis for identifying genetic markers for growth traits for marker-assisted breeding in A. villosum.

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Construction of castor functional markers fingerprint and analysis of genetic diversity

Cited by 3 publications

References 17 publications

Genetic Diversity of Castor Bean (Ricinus communis L.) Revealed by ISSR and RAPD Markers

Genetic Diversity of Castor Bean (Ricinus communis L.) Revealed by ISSR and RAPD Markers

Modern day breeding approaches for improvement of castor

Genome-wide association study of the loci and candidate genes associated with agronomic traits in Amomum villosum Lour

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