Continuous arterial spin labeling of mouse cerebral blood flow using an actively-detuned two-coil system at 9.4T

Lei, Hongxia; Pilloud, Yves; Magill, Arthur W.; Gruetter, Rolf

doi:10.1109/iembs.2011.6091768

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…Because of this, it is standard practice in ASL quantification to assume a BBPC value of 0.9 mL/g based on desiccation experiments performed on ex vivo human brain tissue (Herscovitch and Raichle, 1985; Alsop et al, 2015). This global average value is used for all regions of the brain, all ages and pathologies, and is even adopted when performing ASL in mice (Muir et al, 2008; Lei et al, 2011;Chugh et al, 2012; Gao et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice

2019

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The goal of the study was to develop a novel, rapid Calibrated Short TR Recovery (CaSTRR) method to measure the brain-blood partition coefficient (BBPC) in mice. The BBPC is necessary for quantifying cerebral blood flow (CBF) using tracer-based techniques like arterial spin labeling (ASL), but previous techniques required prohibitively long acquisition times so a constant BBPC equal to 0.9 mL/g is typically used regardless of studied species, condition, or disease. An accelerated method of BBPC correction could improve regional specificity in CBF maps particularly in white matter. Male C57Bl/6N mice ( n = 8) were scanned at 7T using CaSTRR to measure BBPC determine regional variability. This technique employs phase-spoiled gradient echo acquisitions with varying repetition times (TRs) to estimate proton density in the brain and a blood sample. Proton density weighted images are then calibrated to a series of phantoms with known concentrations of deuterium to determine BBPC. Pseudo-continuous ASL was also acquired to quantify CBF with and without empirical BBPC correction. Using the CaSTRR technique we demonstrate that, in mice, white matter has a significantly lower BBPC (BBPC white = 0.93 ± 0.05 mL/g) than cortical gray matter (BBPC gray = 0.99 ± 0.04 mL/g, p = 0.03), and that when voxel-wise BBPC correction is performed on CBF maps the observed difference in perfusion between gray and white matter is improved by as much as 14%. Our results suggest that BBPC correction is feasible and could be particularly important in future studies of perfusion in white matter pathologies.

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Section: Introductionmentioning

confidence: 99%

Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice

2019

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Assessment of Metabolic Fluxes in the Mouse Brain in Vivo Using ¹H-[¹³C] NMR Spectroscopy at 14.1 Tesla

Xin

Lanz

Lei

et al. 2015

J Cereb Blood Flow Metab

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The mathematical model of compartmentalized cerebral metabolism was adapted from previous works 1-3 where the reader can find an exhaustive description of the model.The metabolic pools and fluxes of the considered model are represented in figure 1.Metabolic steady-state was assumed for the metabolic fluxes (constant) and the metabolic pools. The in vivo measured mean glutamate and glutamine concentrations were 9.1 and 3.4 µmol/g, respectively, as measured with

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Skeletal myofiber vascular endothelial growth factor is required for the exercise training‐induced increase in dentate gyrus neuronal precursor cells

Rich

Scadeng

Yamaguchi

et al. 2017

The Journal of Physiology

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Exercise signals neurogenesis in the dentate gyrus of the hippocampus. This phenomenon requires vascular endothelial growth factor (VEGF) originating from outside the blood-brain barrier, but no cellular source has been identified. Thus, we hypothesized that VEGF produced by skeletal myofibers plays a role in regulating hippocampal neuronal precursor cell proliferation following exercise training. This was tested in adult conditional skeletal myofiber-specific VEGF gene-ablated mice (VEGF ) by providing VEGF and non-ablated (VEGF ) littermates with running wheels for 14 days. Following this training period, hippocampal cerebral blood flow (CBF) was measured by functional magnetic resonance imaging (fMRI), and neuronal precursor cells (BrdU+/Nestin+) were detected by immunofluorescence. The VEGF trained group showed improvements in both speed and endurance capacity in acute treadmill running tests (P < 0.05). The VEGF group did not. The number of proliferating neuronal precursor cells was increased with training in VEGF (P < 0.05) but not in VEGF mice. Endothelial cell (CD31+) number did not change in this region with exercise training or skeletal myofiber VEGF gene deletion. However, resting blood flow through the hippocampal region was lower in VEGF mice, both untrained and trained, than untrained VEGF mice (P < 0.05). An acute hypoxic challenge decreased CBF (P < 0.05) in untrained VEGF , untrained VEGF and trained VEGF mice, but not trained VEGF mice. VEGF , but not VEGF , mice were able to acutely run on a treadmill at an intensity sufficient to increase hippocampus VEGF levels. These data suggest that VEGF expressed by skeletal myofibers may directly or indirectly regulate both hippocampal blood flow and neurogenesis.

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Continuous arterial spin labeling of mouse cerebral blood flow using an actively-detuned two-coil system at 9.4T

Cited by 4 publications

References 19 publications

Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice

Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice

Assessment of Metabolic Fluxes in the Mouse Brain in Vivo Using ¹H-[¹³C] NMR Spectroscopy at 14.1 Tesla

Skeletal myofiber vascular endothelial growth factor is required for the exercise training‐induced increase in dentate gyrus neuronal precursor cells

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Continuous arterial spin labeling of mouse cerebral blood flow using an actively-detuned two-coil system at 9.4T

Cited by 4 publications

References 19 publications

Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice

Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice

Assessment of Metabolic Fluxes in the Mouse Brain in Vivo Using 1H-[13C] NMR Spectroscopy at 14.1 Tesla

Skeletal myofiber vascular endothelial growth factor is required for the exercise training‐induced increase in dentate gyrus neuronal precursor cells

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Assessment of Metabolic Fluxes in the Mouse Brain in Vivo Using ¹H-[¹³C] NMR Spectroscopy at 14.1 Tesla