Continuous extraction of proteins with a miniaturized electrical split-flow cell equipped with suspended splitters fabricated by dry film lamination

Capuano, Andrea; Mulloni, Viviana; Adami, Andrea; Lorenzelli, Leandro

doi:10.1016/j.snb.2018.06.082

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…Comparing with microfluidic capillary zone electrophoresis, unfortunately, continuous µFFE inevitably increases the complexity of device fabrication, induces additional stream broadening, and impairs separation performance [8]. Currently, the research field of µFFE focuses on theoretical prediction [9,10], device fabrication [11,12], electrode material selection [13,14], separation mode discussion [15,16], and application expansion [17][18][19][20]. All these efforts will promote the development of µFFE technique and the application of microfluidics in complicated real sample analysis.…”

Section: Introductionmentioning

confidence: 99%

A microchip device to enhance free flow electrophoresis using controllable pinched sample injections

et al. 2019

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Micro free flow electrophoresis (µFFE) is a valuable technique capable of high throughput rapid microscale electrophoretic separation along with mild operating conditions. However, the stream flow separation nature of free flow electrophoresis affects its separation performance with additional stream broadening due to sample stream deflection. To reduce stream broadening and enhance separation performance of µFFE, we presented a simple microfluidic device that enables injection bandwidth control. A pinched injection was formed in the reported µFFE system using operating buffer at sample flow rate ratio (r) setting. Initial bandwidth at the entrance of separation chamber can be shrunk from 800 to 30 µm when r increased from 1 to 256. Stream broadening at the exit of separation chamber can be reduced by about 96% when r increased from 4 to 128, according to both theoretical and experimental results. Moreover, the separation resolution for a dye mixture was enhanced by a factor of 4 when r increased from 16 to 128, which corresponded to an 80% reduction in sample initial bandwidth. Furthermore, a similar enhancement on amino acids separation was obtained by using injection control in the reported µFFE device and readily integrated into online/offline sample preparation and/or downstream analysis procedures.

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Section: Introductionmentioning

confidence: 99%

A microchip device to enhance free flow electrophoresis using controllable pinched sample injections

et al. 2019

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“…[14][15][16][17][18][19][20] Moreover, Tsai et al 21) demonstrated the applying DFR microfluidic channels in microchip capillary electrophoresis. The DFR microfluidic patterns were transferred to glass substrates by the wet etching method for microfluidic devices, as presented by Zhang et al 22) The DFR micropatterns have been manufactured by photolithography, [23][24][25] UV-LEDs (light-emitting diodes) direct writing lithography, 26) direct projection lithography, 27) reactive ion etching and laser ablation, 28) and microscopebased maskless micropatterning technique. 16) However, according to the authors' best knowledge, the DFR has never been applied with the PBW.…”

Section: Introductionmentioning

confidence: 99%

Utilizing a photosensitive dry film resist in proton beam writing

SEKI¹,

Kawamura²,

Hayashi³

et al. 2022

Jpn. J. Appl. Phys.

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Dry film resists (DFRs) are suitable for the fabrication of large volume devices as the thickness of the film can be easily controlled. Here, the DFR microstructures were manufactured using the proton beam writing (PBW) technique by taking advantage of direct writing, beam directivity, and processing depth. The results show that the required irradiation dose of 15 μm DFR was 10 nC/mm2 for 1 MeV protons. In summary, we have optimized the PBW conditions to create smooth surface micropatterns with a vertical wall in the DFR.

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