Continuous lipolysis reactor with a loop connecting an immobilized lipase column and an oil‐water separator

Kosugi, Yoshitsugu; Tomizuka, Noboru

doi:10.1007/bf02546207

Cited by 7 publications

(3 citation statements)

References 12 publications

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“…In der Literatur lassen sich Konzepte finden, in denen im Falle mehrphasiger Reaktionssysteme ausschließlich eine der flüssigen Phasen durch das Festbett geleitet wird. An dieser Stelle sei auf die Arbeiten von Shimizu et al 10 und Kosugi et al 11 verwiesen. Eine schematische Darstellung der sich aus dieser Idee ergebenden zwei Betriebsweisen ist in Abb.…”

Section: Experimentelle Methodikunclassified

Ganzheitliche Analyse dreiphasiger enzymatischer Reaktionssysteme mittels kontinuierlicher Flüssig‐Flüssig‐Phasentrennung

Zitzewitz

Fieg

2015

Chemie Ingenieur Technik

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Die Hydrolyse von Methyloctanoat mittels immobilisierter Candida antarctica Lipase B wurde als beispielhaftes Referenzsystem aus dem Bereich der enzymatischen Flüssig‐Flüssig‐Fest‐Systeme betrachtet. Die experimentelle Untersuchung des Systems erfolgte in einem innovativen Kreislauffestbettreaktor mit kontinuierlicher Flüssig‐Flüssig‐Phasentrennung. Dieser Aufbau erlaubt sowohl einen zwei‐ als auch einen einphasigen Reaktorzulauf. Erstmals erfolgte die ganzheitliche experimentelle Untersuchung beider Betriebsweisen bei verschiedensten Betriebsbedingungen. Neben der Darstellung der experimentellen Ergebnisse werden die Grundzüge der mathematischen Modellierung des Prozesses gezeigt und eine Modellvalidierung vorgenommen.

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Section: Experimentelle Methodikunclassified

Ganzheitliche Analyse dreiphasiger enzymatischer Reaktionssysteme mittels kontinuierlicher Flüssig‐Flüssig‐Phasentrennung

Zitzewitz

Fieg

2015

Chemie Ingenieur Technik

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“…Under these high-temperature conditions the products become discoloured and both the fatty acids and the glycerol may have to be distilled. Enzymic hydrolysis can be effected under milder conditions with a wide range of lipases including Rhizomucor miehei, 20 Aspergillus sp, 21 Pseudomonas uorescens, 22 Candida rugosa, 20 and Candida cylindracea 23 acting at $37°C for about 24 hours. Highmelting fats can be hydrolysed under these conditions in the presence of a little iso-octane.…”

Section: Hydrolysismentioning

confidence: 99%

Enzymes as biocatalysts in the modification of natural lipids

Gunstone

1999

J. Sci. Food Agric.

130

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“…However, one of the major problems in hydrolysis by lipases is that the catalytic activity of the enzyme occurs in a heterogeneous system containing water and insoluble substrate. The biphasic process, or the organic two-phase system in which the fat or oil is dissolved in an organic solvent and hydrolyzed by lipases, has been investigated by many scientists (2)(3)(4)(5)(6)(7).…”

mentioning

confidence: 99%

Continuous production of fatty acids from palm olein by immobilized lipase in a two‐phase system

H‐Kittikun

Prasertsan

Sungpud

2000

J Americ Oil Chem Soc

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Commercial lipases were tested for the ability to hydrolyze palm olein in isooctane in a two-phase system. Lipase OF (from Candida rugosa) showed the highest specific activity of 209 U/mg protein where 1 U is the amount of lipase enzyme required to produce 1 µmol of fatty acid (as palmitic acid) per minute. The enzyme was adsorbed completely on Accurel EP100 (particle size <200 µm) with 20.5% activity retained. The soluble and the immobilized lipase OF showed optimal activity at the same pH and temperature (pH 6.5-7.5 and 35°C). However, the immobilized lipase had a wider range of pH and higher temperature stability. Continuous hydrolysis of palm olein was performed in a packed-bed reactor with 656 U of immobilized enzyme. The substrate (20% palm olein in isooctane) and Tris/maleate buffer were fed concurrently at the flow rates of 0.08 and 0.04 mL/min, respectively. The system gave a degree of hydrolysis (DH) of 90-100% for up to 250 h. A more stable system allowing for more than 300 h operation at DH > 95% was achieved by mixing the immobilized enzyme with 1000-1500 µm Accurel EP100 to increase the system porosity and continuous feeding of the aqueous phase recycling from the product mixture. A similar result was also obtained using 1007 U of the immobilized enzyme and 60% palm olein in isooctane fed at 0.06 mL/min.

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Continuous lipolysis reactor with a loop connecting an immobilized lipase column and an oil‐water separator

Cited by 7 publications

References 12 publications

Ganzheitliche Analyse dreiphasiger enzymatischer Reaktionssysteme mittels kontinuierlicher Flüssig‐Flüssig‐Phasentrennung

Ganzheitliche Analyse dreiphasiger enzymatischer Reaktionssysteme mittels kontinuierlicher Flüssig‐Flüssig‐Phasentrennung

Enzymes as biocatalysts in the modification of natural lipids

Continuous production of fatty acids from palm olein by immobilized lipase in a two‐phase system

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