Continuous production of acetone-butanol-ethanol using immobilized cells of Clostridium acetobutylicum and integration with product removal by liquid-liquid extraction

Continuous two stage acetone–butanol–ethanol fermentation with integrated solvent removal using Clostridium acetobutylicum B 5313

“…In batch fermentation, reactor productivity is in the order of 0.35-0.40 g/L.h (Qureshi and Maddox, 1995). Such low reactor productivity requires large reactor volumes which results in high capital and operational costs.…”

“…Therefore, an optimized dilution rate plays an important role in ABE fermentation. Lower concentrations of substrate shifts the system into acidogenic phase rather than solventogenic phase and lowers the solvent yield (Qureshi and Maddox, 1995). Hence consumption of substrate beyond 85% is not desirable for higher yields.…”

Biobutanol from Renewable Agricultural and Lignocellulose Resources and Its Perspectives as Alternative of Liquid Fuels

“…When operated in an integrated system with product removal by liquid-liquid extraction, there was a decrease in productivity, but lactose utilization was increased markedly. Of the three extractants tested, oleyl alcohol proved to be superior to both benzyl benzoate and dibutyl phthalate [214].…”

Production of Butyric Acid and Butanol from Biomass

“…In the past 20+ years, there have been numerous engineering attempts to improve butanol production in ABE fermentation, including using cell recycle and cell immobilization to increase cell density and reactor productivity and using extractive fermentation to minimize product inhibition (Geng and Park, 1994;Maddox et al, 1995;Mulchandani and Volesky, 1994;Park et al, 1990;Qureshi and Maddox, 1995;Yang and Tsao, 1995). For example, ABE fermentation with cell recycle using a spin filter perfusion bioreactor, 49 g/L of cell mass was achieved and the process gave a butanol productivity of 1.14 g/L/h.…”