Continuous Production of Butanol by Clostridium acetobutylicum Immobilized in a Fibrous Bed Bioreactor

Continuous two stage acetone–butanol–ethanol fermentation with integrated solvent removal using Clostridium acetobutylicum B 5313

“…In continuous ABE fermentation, as the solventogenic Clostridia cells continue to undergo shifts between acidogenesis and solventogenesis, the bioreactor houses a mixture of actively dividing cells (acidogenic), non-dividing cells (solventogenic), sporulated cells, and dead cells. The oscillatory behavior of the ABE concentration in the bioreactor and effluent during the continuous ABE fermentation by C. acetobutylicum indicates that there are periods of time when the non-dividing cells (solventogenic) concentration is comparatively high or low in the bioreactor resulting in high or low ABE production (Lai and Traxler, 1994;Mulchandani and Volesky, 1994;Huang et al, 2004).…”

Biobutanol from Renewable Agricultural and Lignocellulose Resources and Its Perspectives as Alternative of Liquid Fuels

“…At a dry cell mass concentration of 8 g/L and a dilution rate of D=0.64 h [108]. Huang and Ramey [109] determined the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum in a fibrous bed bioreactor with high cell density and butyrate concentrations at pH 5.4 and 35°C. By feeding glucose and butyrate as cosubstrates, the fermentation was maintained in the solventogenesis phase, and the optimal butanol productivity of 4.6 g L were obtained at a dilution rate of 0.9 h -1 and pH 4.3.…”

Production of Biofuels from Cellulose of Woody Biomass

“…In immobilized cell fermentation, cells are stationary, which have the advantages of the long survival time of cells in solventogenic phase. In one study, immobilized cell fermentation using C. acetobutylicum produced 20% higher butanol yield than did free cell fermentation [90]. However, the scale up process using immobilized technology seems to have technical problems due to excessive cell growth in the packed beds and blockage [85].…”