Contractile Potentiation by Endothelin-1 Involves Protein Kinase C-.DELTA. Activity in the Porcine Coronary Artery.

Obara, Kazuo; Hata, Shinzo; Sato, Katsura; Koide, Masayo; Ishii, Kunio; Nakayama, Koichi

doi:10.2170/jjphysiol.49.175

Cited by 21 publications

(21 citation statements)

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“…6: A -C). In addition, positive staining for PKCδ and PKCε, which have been suggested to be involved in vascular contraction (14,15), was also observed (Fig. 6: D and E).…”

Section: Immunohistochemistry For Pkc Isoformsmentioning

confidence: 78%

Distinct Roles of Protein Kinase C Isoforms in Myogenic Constriction of Rat Posterior Cerebral Arteries

Kashihara¹,

Nakayama²,

Ishikawa³

2008

J Pharmacol Sci

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Abstract. The involvement of protein kinase C (PKC) in myogenic constriction at physiological intraluminal pressure was investigated in rat posterior cerebral arteries. ] i elevation gradually declined in the presence of rottlerin, and it was nearly abolished by ruthenium red. Immunohistochemical analysis showed positive staining for PKCα, γ, δ, and ε, but not PKCβ, in smooth muscle cells of rat posterior cerebral arteries. These results suggest distinct roles of PKC isoforms in myogenic constriction: conventional PKC mediates Ca 2+ sensitization, whereas PKCδ mediates sustained [Ca 2+ ] i elevation via the activation of cation channels, resulting in sustained constriction.

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“…6: A -C). In addition, positive staining for PKCδ and PKCε, which have been suggested to be involved in vascular contraction (14,15), was also observed (Fig. 6: D and E).…”

Section: Immunohistochemistry For Pkc Isoformsmentioning

confidence: 78%

Distinct Roles of Protein Kinase C Isoforms in Myogenic Constriction of Rat Posterior Cerebral Arteries

Kashihara¹,

Nakayama²,

Ishikawa³

2008

J Pharmacol Sci

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“…22 We reported that ␣-, ␤ 1 -, ␦, and -isoforms are expressed in the porcine coronary artery. 22 Coronary segments from the spastic and control sites were dissected and cut into rings approximately 4 mm in length. Western blotting for PKC isoforms was performed.…”

Section: Western Blot Analysis For Membrane Translocation Of Pkc Isofmentioning

confidence: 93%

“…Western blotting for PKC isoforms was performed. 22 As a control, one ring was frozen after incubation in PSS, and as an activated preparation, another ring stimulated with serotonin (1 mol/L) was frozen 5 minutes after exposure to the monoanine. These segments were minced with scissors into small pieces in ice-cold buffer, homogenized with a Polytron on ice, and then centrifuged at 100 000g for 30 minutes at 4°C.…”

Section: Western Blot Analysis For Membrane Translocation Of Pkc Isofmentioning

confidence: 99%

“…Tissue extracts were subjected to SDS-PAGE as previously described. 22 The antibodies used included rabbit PKC isoform-specific primary antibodies (Sigma, St. Louis, Mo, and Research & Diagnostic Antibodies, Berkley, Calif) and mouse PKC isoform-specific primary antibodies (Transduction Laboratory, Lexington, Ky). The extent of translocation of each PKC isoform from the cytosol to membrane fraction was used as a marker of the enzyme activity.…”

Section: Western Blot Analysis For Membrane Translocation Of Pkc Isofmentioning

confidence: 99%

“…The extent of translocation of each PKC isoform from the cytosol to membrane fraction was used as a marker of the enzyme activity. 22 …”

Section: Western Blot Analysis For Membrane Translocation Of Pkc Isofmentioning

confidence: 99%

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Evidence for Protein Kinase C-Mediated Activation of Rho- Kinase in a Porcine Model of Coronary Artery Spasm

Kimura

Shimokawa

Miyata

et al. 2003

ATVB

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Objective-We have recently demonstrated that protein kinase C (PKC) and Rho-kinase play important roles in coronary vasospasm in a porcine model. However, it remains to be examined whether there is an interaction between the two molecules to cause the spasm. Methods and Results-A segment of left porcine coronary artery was chronically treated with IL-1␤-bound microbeads in vivo. Two weeks after the operation, phorbol ester caused coronary spasm in vivo and coronary hypercontractions in vitro at the IL-1␤-treated segment; both were significantly inhibited by hydroxyfasudil, a specific Rho-kinase inhibitor. Guanosine 5Ј-[␥-thio]triphosphate (GTP␥S), which activates Rho with a resultant activation of Rho-kinase, enhanced Ca 2ϩ sensitization of permeabilized vascular smooth muscle cells, which were resistant to the blockade of PKC by calphostin C. The GTP␥S-induced Ca 2ϩ sensitization was greater in the spastic segment than in the control segment. Western blot analysis revealed that only PKC␦ isoform was activated during the hypercontraction.Conclusions-These results demonstrate that PKC and Rho-kinase coexist on the same intracellular signaling pathway, with PKC located upstream on Rho-kinase, and that among the PKC isoforms, only PKC␦ may be involved. Thus, the strategy to inhibit Rho-kinase rather than PKC may be a more specific and useful treatment for coronary spasm. Key Words: arteriosclerosis Ⅲ coronary disease Ⅲ smooth muscle Ⅲ signal transduction C oronary artery spasm plays an important role in the pathogenesis of a variety of ischemic heart diseases, including not only variant angina but also other forms of angina, myocardial infarction, ventricular arrhythmias, and sudden death. 1,2 However, the intracellular mechanism for the spasm remains to be elucidated.We previously developed a porcine model of coronary spasm in which the spasm was repeatedly induced by autacoids, such as serotonin and histamine, at atherosclerotic lesions made by a combination of balloon endothelial removal and high-cholesterol feeding. [3][4][5] We subsequently developed a porcine model in which long-term adventitial treatment with interleukin-1␤ (IL-1␤), one of the major inflammatory cytokines, induces arteriosclerotic changes and vasospastic responses of the coronary artery. 6 -15 Since the histological changes and vasospastic responses in our porcine models are similar to those observed in humans, our models may be useful to elucidate the mechanisms of the spasm in humans.We recently demonstrated that in our porcine models, coronary artery spasm induced by serotonin is mediated by protein kinase C (PKC) 5,7 and Rho-kinase. 9,11-15 Rho-kinase, one of the major target proteins of small Rho GTPase, 16,17 was upregulated in spastic coronary segment, leading to an increased phosphorylation of myosin-binding subunit of myosin phosphatase (MLCPh) and the resultant suppression of MLCPh. 11-15 Indeed, we recently demonstrated that Rho-kinase is substantially involved in hypercontractions of isolated arteriosclerotic human arteries in v...

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Glycated Serum Albumin-Induced Vascular Smooth Muscle Cell Proliferation through Activation of the Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Pathway by Protein Kinase C

Hattori

Kakishita

Akimoto

et al. 2001

Biochemical and Biophysical Research Communications

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Contractile Potentiation by Endothelin-1 Involves Protein Kinase C-.DELTA. Activity in the Porcine Coronary Artery.

Cited by 21 publications

References 0 publications

Distinct Roles of Protein Kinase C Isoforms in Myogenic Constriction of Rat Posterior Cerebral Arteries

Distinct Roles of Protein Kinase C Isoforms in Myogenic Constriction of Rat Posterior Cerebral Arteries

Evidence for Protein Kinase C-Mediated Activation of Rho- Kinase in a Porcine Model of Coronary Artery Spasm

Glycated Serum Albumin-Induced Vascular Smooth Muscle Cell Proliferation through Activation of the Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Pathway by Protein Kinase C

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