Contribution of ferrous iron to maintenance of the gastric colonization of Helicobacter pylori in miniature pigs

Koga, Tetsufumi; Shimada, Yukio; Satô, Kiyoshi; Takahashi, Keigo; Kikuchi, Isamu; Okazaki, Yoko; Miura, Tomihiro; Katsuta, Mitsuo; Iwata, Masayuki

doi:10.1078/0944-5013-00169

Cited by 5 publications

(8 citation statements)

References 29 publications

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“…The requirement for ample amounts of dietary iron is supported by an in vivo study by Koga and colleagues (12). Those authors found that improved colonization of piglets by H. pylori occurred when the animals' diet was supplemented with additional iron (12). The high-iron requirement of H. pylori could also explain why this organism does not colonize the intestine, where other bacterial species would rapidly scavenge iron.…”

Section: Discussionmentioning

confidence: 97%

Nutritional Requirements and Antibiotic Resistance Patterns ofHelicobacterSpecies in Chemically Defined Media

et al. 2006

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The growth of the gastric pathogen Helicobacter pylori in the absence of serum remains challenging, and nutritional requirements have only partially been defined, while almost nothing is known about nutritional requirements of other Helicobacter spp. Although previous data showed that H. pylori grows in the chemically defined medium F-12, but not in other tissue culture media examined, the specific components responsible for growth were not entirely understood. Here we describe the optimization of amino acids, metals, and sodium chloride for H. pylori. Iron, zinc, and magnesium were critical for growth; copper was not required. Optimization of sodium chloride was further beneficial. Nutritional requirements and antibiotic resistance patterns of several other Helicobacter spp. revealed that all except H. felis grew in serum-free, unsupplemented F-12. All Helicobacter spp. were resistant to at least six antimicrobial agents when cultured in the presence of serum. However, in the absence of serum, H. pylori, H. mustelae, and H. muridarum became sensitive to polymyxin B and/or trimethoprim. Much of the data were obtained using a convenient ATP assay to quantify growth. H. pylori has surprisingly few absolute requirements for growth: 9 amino acids, sodium and potassium chloride, thiamine, iron, zinc, magnesium, hypoxanthine, and pyruvate. These data suggest that H. pylori and other Helicobacter spp. are not as fastidious as previously thought. The data also suggest that chemically defined media described herein could yield the growth of a wide range of Helicobacter spp., allowing a more detailed characterization of Helicobacter physiology and interactions with host cells.

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Section: Discussionmentioning

confidence: 97%

Nutritional Requirements and Antibiotic Resistance Patterns ofHelicobacterSpecies in Chemically Defined Media

et al. 2006

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“…Although, there are differences between naturally occurring gastric ulcers between pigs and humans, the pig represents an interesting animal model to study the repair of gastric ulcers (KOGA et al, 2002;PADRA et al, 2018;BANG et al, 2019;MAEDA et al, 2019), and the pathogenic mechanisms involved in their formation, mainly with Helicobacter pylori (POUTAHIDIS et al, 2001). Pigs have systemic similarities with humans and a suitable profile for biomedical research (GIANOTTI et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Helicobacter spp. and gastric lesions association in minipigs

et al. 2021

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Due to their similarity to humans, studies regarding gastric ulcers in pigs have become of great interest. It is known that Helicobacter spp. is related to the occurrence of these ulcers, as they possess a high prevalence in pigs. This study aimed to associate gastric lesions findings naturally occurring in minipigs related to or not related to the presence of Helicobacter spp., through the ultra-rapid urease test and immunohistochemical analysis, reaffirming the disease in swine as a natural biomedical model for human cases. For this, samples of formalin-fixed paraffin-embedded gastric tissues from 40 minipigs were obtained at the Laboratório de Morfologia e Patologia Animal (LMPA) in the Universidade Estadual do Norte Fluminense (UENF). Regarding the ultra-rapid urease test, no animal was positive in all regions. However, 18 were positive in at least one: six (15%) were positive in the aglandular region, eight (20%) in the antrum region, 13 (32.5%) in the region of the cardiac gland, and one (2.5%) in the region of the fundic gland. Regarding immunohistochemical analysis, only one animal was positive in all regions, and 32 animals were positive in at least one: four (10%) were positive in the aglandular region, 10 (25%) in the antrum region, 23 (57.5%) in the region of the cardiac gland, and three (7.5%) in the region of the fundic gland. The gastric lesion findings showed a close relationship with Helicobacter spp., enriching the laboratory animal pathologies list. The immunostaining of the bacteria not associated with gastric lesions in certain regions demonstrated the saprophytic and opportunisitic nature of Helicobacter.

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“…[161][162][163][164][165][166][167][168] However, inflammation and gastritis were observed in the early stages of H. pylori infection (below 1 year). [160][161][162][163][164][165][166][167][169][170][171][172][173] Still, after 5 years of H. pylori inoculation, Neoplasia was observed. 168 Besides nonhuman primates, pigs were also used as an animal model in gastric cancer research, and they showed diverse pathological outcomes due to H. pylori infection.…”

Section: Other Animalsmentioning

confidence: 99%

A comprehensive evaluation of an animal model for Helicobacter pylori‐associated stomach cancer: Fact and controversy

et al. 2023

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Even though Helicobacter pylori infection was the most causative factor of gastric cancer, numerous in vivo studies failed to induce gastric cancer using H. pylori infection only. The utilization of established animal studies in cancer research is crucial as they aim to investigate the coincidental association between suspected oncogenes and pathogenesis as well as generate models for the development and testing of potential treatments. The methods to establish gastric cancer using infected animal models remain limited, diverse in methods, and showed different results. This study investigates the differences in animal models, which highlight different pathological results in gaster by literature research. Electronic databases searched were performed in PubMed, Science Direct, and Cochrane, without a period filter. A total of 135 articles were used in this study after a full‐text assessment was conducted. The most frequent animal models used for gastric cancer were Mice, while Mongolian gerbils and Transgenic mice were the most susceptible model for gastric cancer associated with H. pylori infection. Additionally, transgenic mice showed that the susceptibility to gastric cancer progression was due to genetic and epigenetic factors. These studies showed that in Mongolian gerbil models, H. pylori could function as a single agent to trigger stomach cancer. However, most gastric cancer susceptibilities were not solely relying on H. pylori infection, and numerous factors are involved in cancer progression. Further study using Mongolian gerbils and Transgenic mice is crucial to conduct and establish the best models for gastric cancer associated H. pylori.

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Contribution of ferrous iron to maintenance of the gastric colonization of Helicobacter pylori in miniature pigs

Cited by 5 publications

References 29 publications

Nutritional Requirements and Antibiotic Resistance Patterns ofHelicobacterSpecies in Chemically Defined Media

Nutritional Requirements and Antibiotic Resistance Patterns ofHelicobacterSpecies in Chemically Defined Media

Helicobacter spp. and gastric lesions association in minipigs

A comprehensive evaluation of an animal model for Helicobacter pylori‐associated stomach cancer: Fact and controversy

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