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Gram-stain-negative, aerobic, halophilic bacteria, designated SCM-1 T , LCM10-1 and CTBL-B-147, were isolated from modified half-strength SWM-III medium, PES medium and thalli after laboratory cultivation of a red alga, Porphyra yezoensis. Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolates were affiliated to the genus Sulfitobacter of the class Alphaproteobacteria, and the 16S rRNA gene sequence similarity of the new isolates with the closest related species, Sulfitobacter mediterraneus CH-B427 The genus Sulfitobacter of the family Rhodobacteraceae in the class Alphaproteobacteria was described by Sorokin (1995) and currently includes nine species (http://www. bacterio.net/) that have been isolated from the H 2 S-O 2 interface of the Black Sea (Sorokin, 1995), a hyper-saline lake (Labrenz et al., 2000), starfish and sea grass (Ivanova et al., 2004). During the taxonomic investigation of the bacterial population in a laboratory-cultured red alga, Porphyra yezoensis, three bacteria designated SCM-1 T , LCM10-1 and CTBL-B-147 were isolated. In this study, we describe the polyphasic taxonomic study of the new isolates.Thalli of P. yezoensis U-51 were cultured in sterile, modified half-strength SWM-III medium (Ogata, 1970) at 17 u C for 5 weeks or in sterile PES medium (Provasoli, 1968) at 18 u C for 6 weeks. Samples of the modified halfstrength SWM-III medium, PES medium and thalli were plated on marine agar 2216 (MA; BD) and 10-fold diluted MA (1/10 MA), and then incubated at 20 u C for 2 weeks. Strain SCM-1 T was isolated on MA from the modified half-strength SWM-III medium, and strains LCM10-1 and CTBL-B-147 were isolated on 1/10 MA and MA from the thalli cultured in modified half-strength SWM-III Abbreviations: FAME, fatty acid methyl ester; ML, maximum-likelihood; MP, maximum-parsimony; NJ, neighbour-joining.

Our previous study showed that the colonization levels of Helicobacter pylori were higher in the stomachs of 5-day-old miniature pigs than in 2-week-old ones. As dietary factors can cause these differences, we compared two diets, i.e., Weanymilk and a similar formula with a higher concentration of Fe(II), Weanylobulin. The colonization levels in the fundic mucosa were significantly higher in 2-week-old pigs fed Weanylobulin than in those fed Weanymilk. Supplementing Weanylobulin with an iron chelator, deferoxamine mesylate, significantly lowered the bacteria counts in the gastric mucosa. Normal diets supplemented with Fe(II) in 2-month-old pigs caused significantly more sites of bacteria in the antrum compared with normal diets alone. In addition, ranitidine, an inhibitor of gastric acid secretion that reduces Fe(III) to Fe(II) in the stomach, decreased the bacteria counts in 10-month-old pigs. These results suggested that Fe(II) maintained the colonization levels of H. pylori in the stomach of the miniature pigs.

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